Functional analysis of fungal effector candidate ssp (small secreted protein) genes of septoria leaf blotch pathogen (zymoseptoria tritici) in wheat

Mustafa, Zemran
Wheat (Triticum aestivum L.) is one of the main cereals sown world-wide. It constitutes a significant portion of daily calorie intake in many parts of the world. Despite lots of studies conducted over many years to increase the yield, there are significant losses in wheat production due to biotic and abiotic factors. Septoria tritici blotch is one of the most challenging disease among biotic stress factors. Septoria Leaf Blotch is one of the most important fungal disease in Turkey and the world, causing many concerns in wheat fields. Upon adequate conditions Zymoseptoria tritici causes direct yield loss proportionally to chlorophyll loss on leaves. Primarily, the mechanism of disease formation of the fungus needs to be understood for development of effective strategies against this pathogen. It is known that when the fungus and host plant come across following the infection, pathogens secrete small proteins called effectors. Some of these effectors affect the functioning of plant proteins, suppressing its resistance and facilitating disease formation. In this project, some of the Small Secreted Proteins (SSPs) genes on Z. tritici genome are knocked out via Agrobacterium mediated DNA fragment transfer and utilizing pathogen’s homologous recombination mechanism. The effect of the knocked-out genes in fungal virulence were investigated using classical virulence tests on these gene knock out mutant isolates. Also, development process of mutant isolates on synthetic media were monitored to investigate effects of the deleted gene on phenotypic features of the fungi.


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Baloğlu, Mehmet Cengiz; Yücel, Ayşe Meral; Öktem, Hüseyin Avni; Department of Biology (2011)
Wheat is the most important grain crop grown in our country providing greatest part of the daily nutritional requirement. Abiotic factors including salinity, drought, cold and heat stresses affect quality and yield of wheat varieties used for the production of both bread and pasta flour. NAC proteins form one of the widest families of plant specific transcription factors. Members of this family are related with development, defense and abiotic stress responses. TaNAC69-1 and TtNAM-B2 genes were isolated fro...
Phylogenetic relationships among Triticum L. and Aegilops L. species as genome progenitors of bread wheat based on sequence diversity in trnT-F region of chloroplast DNA
Dizkirici, Ayten; Kansu, Cigdem; Önde, Sertaç; AVCI BİRSİN, MELAHAT; Ozgen, Murat; Kaya, Zeki (Springer Science and Business Media LLC, 2013-12-01)
Cultivated wheat, (Triticum aestivum L.), is one of the most important food crops in the world. The Aegilops L. genus is frequently utilized by plant breeders for improving the current wheat cultivars due to their close relationships. Therefore, understanding the phylogenetic relationships among the species of these genera is not only valuable for plant taxonomy, but also for plant breeding efforts. The presented phylogenetic analysis was based on the sequences of trnT-F chloroplast DNA containing three non...
Hydrolysis of freshly prepared wheat starch fractions and commercial wheat starch using α-amylase
Sakintuna, Billur; Budak, Okan; Dik, Tunay; Yöndem Makascıoğlu, Füsun; Kincal, N. Suzan (Informa UK Limited, 2003-5)
The enzymic hydrolysis of commercial wheat starch and freshly prepared wheat starch fractions was studied in batch and flow systems. Fresh starch was prepared by wet separation of wheat flour into starch milk and gluten, followed by processing the starch milk using a serially connected hydrocyclone system to produce underflow and overflow streams. The underflow stream consisted mainly of the larger (> 30 w m) granules, while the overflow stream contained only the smaller (< 10 w m) granules. In batch system...
Genetic screening of Turkish wheat varieties for the durable resistance gene, Lr34
Boylu, Barış; Akkaya, Mahinur S.; Hakkı, Erdoğan Eşref; Department of Biotechnology (2011)
Wheat diseases such as rusts and powdery mildews are among the most important and ancient diseases that affect wheat cultivation worldwide. The pathogen race specific resistance genes cannot maintain long lasting resistance. On the other hand, the presence of genes confers the non-race specific resistance last much longer. The durable resistance phenotypes in wheat against various rust and powdery mildew diseases were reported as Lr34, Yr18, and Pm38 separately; nevertheless, they were known to locate very ...
Optimisation of tissue culture, regeneration and Agrobacterium-mediated transformation paramaters in winter wheat cultivars (T.durum cv. Kiziltan-91 and T.aestivum cv. Bezostaja-01)
Kavas, M.; Öktem, Hüseyin Avni; YÜCEL, MUSTAFA (2006-08-18)
Wheat, like other cereal species, shows a common recalcitrance and strong genotypic variation in tissue culture and Agrobacteriummediated transformation. A variety of explant sources have been used for obtaining wheat plants from in vitro cultures and Agrobacterium- mediated transformation. These are: isolated microspores (Mejza et al., 1993), mature embryos (Özgen et al., 1998; Delporte et al., 2001), immature embryos (Ozias-Akins and Vasil, 1982; Vasil et al., 1990; Pellegrineschi et al., 2004), and immat...
Citation Formats
Z. Mustafa, “Functional analysis of fungal effector candidate ssp (small secreted protein) genes of septoria leaf blotch pathogen (zymoseptoria tritici) in wheat,” Ph.D. - Doctoral Program, Middle East Technical University, 2019.