TILLING for TtBH-1 gene and its hybridization to Turkish durum wheat cultivars Kızıltan-91 and Fuatbey-2000

Battal, Abdulhamit
Wheat is one of the main crops to get daily calory requirement for people. Improving wheat yield is one of the most important aims in crop research today. Spike structure genes play a crucial role for yield increase. TtBH-A1 gene has a single nucleotide polymorphism at the conserved domain causing branched spikes producing more spikelet, grains and yield in “Miracle-Wheat”. In this study, TtBH-1 homoeologous (TtBH-A1 and TtBH-B1) gene were screened in tetraploid Kronos Targeting Induced Local Lesions In Genomes (TILLING) population. 80 new alleles were confirmed for TtBH-1 gene. One mutation leading to a premature termination codon was validated for the TtBH-B1 gene in line T4-2432 (Q14*), while a mutation in a conserved domain was validated for TtBH-A1 gene. Glycine at the position 61 of TtBH-A1 protein was converted to serine as a result of mutation of guanine at the position 181 of the gene to adenine in line T4-2447. Supernumerary spikelets were observed in the A-genome homozygous mutant as short branches, whereas no effects were observed in the B genome mutants. Lateral spikelet development leading to additional spikelet side of the normal spikelet was initiated during glume promordium formation according to immature inflorescence observations. T4-2447 mutants produced significantly more spikelet than Kronos . A genome and B genome mutants were combined to make double mutants. TtBH-1 mutations was backcrossed into parental Kronos to eliminate background mutations. Additionally, T4-2447 mutant line and “Miracle-Wheat” was hybridized with Turkish domestic cultivars Kızıltan-91 and Fuatbey-2000. Yield components were evaluated for T4-2447 mutants.


Expression analysis of NAC type transcription factors on wheat seedlings under abiotic stress conditions
Baloğlu, Mehmet Cengiz; Yücel, Ayşe Meral; Öktem, Hüseyin Avni; Department of Biology (2011)
Wheat is the most important grain crop grown in our country providing greatest part of the daily nutritional requirement. Abiotic factors including salinity, drought, cold and heat stresses affect quality and yield of wheat varieties used for the production of both bread and pasta flour. NAC proteins form one of the widest families of plant specific transcription factors. Members of this family are related with development, defense and abiotic stress responses. TaNAC69-1 and TtNAM-B2 genes were isolated fro...
Genetic screening of Turkish wheat varieties for the durable resistance gene, Lr34
Boylu, Barış; Akkaya, Mahinur S.; Hakkı, Erdoğan Eşref; Department of Biotechnology (2011)
Wheat diseases such as rusts and powdery mildews are among the most important and ancient diseases that affect wheat cultivation worldwide. The pathogen race specific resistance genes cannot maintain long lasting resistance. On the other hand, the presence of genes confers the non-race specific resistance last much longer. The durable resistance phenotypes in wheat against various rust and powdery mildew diseases were reported as Lr34, Yr18, and Pm38 separately; nevertheless, they were known to locate very ...
Site directed mutations of one of the plant pathogen effector gene for functional analysis
Mustafa, Zemran; Akkaya, Mahinur S.; Department of Biochemistry (2015)
Wheat (Triticum aestivum L.) is one of the most important crops planted in the world and used as major food resource. Yet, there are major losses of productivity due to different biotic and abiotic destructive agents. Among the biotic factors especially the yellow rust is one of the most frequent. Yellow rust or also called “stripe rust”, due to stripe like appearances on the leaves, disease is caused by Puccinia striiformis f. sp. tritici, which is an obligate plant fungal pathogen. To cope with high yield...
Particle bombardment transformation of some Turkish wheat cultivars with TaNAC69-1 and TtNAMB2 genes
Battal, A.; BALOĞLU, MEHMET CENGİZ; Kavas, M.; Yücel, Mustafa; Öktem, Hüseyin Avni (2012-09-23)
Wheat is one of the most important crop plants in the world.The production of wheat is seriously affected by biotic (pests,bacterial and fungal diseases) and abiotic (drought, salinity andfreezing) stresses. NAC family proteins (NAM/ATAF1-2/CUC2) areplant specific transcription factors and related with development,defense and abiotic stress responses.TaNAC69-1andTtNAMB2genes are the transcription factors from this protein family. Theaim of this study was to transform of some Turkish ...
Investigating the roles of micrornas in biotic stress responses and functional characterization of a novel ztl-type f-box protein via virus induced gene silencing
Dağdaş, Yasin Fatih; Akkaya, Mahinur S.; Department of Biotechnology (2009)
Barley and wheat are the two most important crop species in Turkey. Molecular studies for increasing crop yield of these species are very important for the economic benefits of Turkey. Powdery mildew and yellow rust are the two main pathogens, infecting barley and wheat, respectively in our country and causing a great amount of yield loss each year. Till now, classical genetics studies were performed in order to develop resistant barley and wheat cultivars, but these studies have not been succesful. Therefo...
Citation Formats
A. Battal, “TILLING for TtBH-1 gene and its hybridization to Turkish durum wheat cultivars Kızıltan-91 and Fuatbey-2000,” Ph.D. - Doctoral Program, Middle East Technical University, 2016.