Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Expression analysis of NAC type transcription factors on wheat seedlings under abiotic stress conditions
Download
index.pdf
Date
2011
Author
Baloğlu, Mehmet Cengiz
Metadata
Show full item record
Item Usage Stats
246
views
277
downloads
Cite This
Wheat is the most important grain crop grown in our country providing greatest part of the daily nutritional requirement. Abiotic factors including salinity, drought, cold and heat stresses affect quality and yield of wheat varieties used for the production of both bread and pasta flour. NAC proteins form one of the widest families of plant specific transcription factors. Members of this family are related with development, defense and abiotic stress responses. TaNAC69-1 and TtNAM-B2 genes were isolated from T.aestivum and T.turgidum, respectively. Then they were cloned into different monocot and dicot expression vectors to be used for further wheat and tobacco genetic transformation studies. To understand effects of salinity, drought, cold and heat stresses on expression profiles of TaNAC69-1 and TtNAM-B2 genes, quantitative real time PCR was performed. The time series expression profiles of TaNAC69-1 show that it was significantly up-regulated following by salt, drought, cold and heat stress treatments. Except for heat stress, expression of TtNAMB-2 gene also significantly induced under drought, salt and cold stress conditions. Microarray analysis also performed to indicate effects of cold and heat stress treatments on global gene expression profiles of wheat. Differentially regulated genes show that temperature changes directly affected a large and complex transcriptional network associated with defense, metabolism and development. Genes involved in cold stress-responsive and different cold acclimation proteins were extremely up-regulated upon exposure to cold stress. Both expression levels of small and large sub-unit heat shock proteins significantly increased following heat stress period. In addition to these protective agents, transcription factors also played a central role to deal with severe effects of low and high temperature conditions.
Subject Keywords
Wheat
URI
http://etd.lib.metu.edu.tr/upload/12613501/index.pdf
https://hdl.handle.net/11511/21170
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Genetic screening of Turkish wheat varieties for the durable resistance gene, Lr34
Boylu, Barış; Akkaya, Mahinur S.; Hakkı, Erdoğan Eşref; Department of Biotechnology (2011)
Wheat diseases such as rusts and powdery mildews are among the most important and ancient diseases that affect wheat cultivation worldwide. The pathogen race specific resistance genes cannot maintain long lasting resistance. On the other hand, the presence of genes confers the non-race specific resistance last much longer. The durable resistance phenotypes in wheat against various rust and powdery mildew diseases were reported as Lr34, Yr18, and Pm38 separately; nevertheless, they were known to locate very ...
TILLING for TtBH-1 gene and its hybridization to Turkish durum wheat cultivars Kızıltan-91 and Fuatbey-2000
Battal, Abdulhamit; Yücel, Ayşe Meral; Eyidoğan, Füsun; Department of Biotechnology (2016)
Wheat is one of the main crops to get daily calory requirement for people. Improving wheat yield is one of the most important aims in crop research today. Spike structure genes play a crucial role for yield increase. TtBH-A1 gene has a single nucleotide polymorphism at the conserved domain causing branched spikes producing more spikelet, grains and yield in “Miracle-Wheat”. In this study, TtBH-1 homoeologous (TtBH-A1 and TtBH-B1) gene were screened in tetraploid Kronos Targeting Induced Local Lesions In Gen...
Determination of genes involved in the yellow rust disease of wheat
Bozkurt, Osman; Kocabıyık, Semra; Department of Biotechnology (2007)
It is important to understand the underlying plant defense mechanisms in order to establish best strategies to reduce losses due to diseases in cereals. The current available information is mostly on model organisms and their plant-pathogen interactions. However, this study is focused on the identification of genes involved in the resistance mechanism of one of the most devastating diseases of wheat, yellow rust. The strategy undertaken was to use differential display method (DD) together with microarray te...
Functional analysis of fungal effector candidate ssp (small secreted protein) genes of septoria leaf blotch pathogen (zymoseptoria tritici) in wheat
Mustafa, Zemran; Akkaya, Mahinur S.; Ölmez, Fatih; Department of Biotechnology (2019)
Wheat (Triticum aestivum L.) is one of the main cereals sown world-wide. It constitutes a significant portion of daily calorie intake in many parts of the world. Despite lots of studies conducted over many years to increase the yield, there are significant losses in wheat production due to biotic and abiotic factors. Septoria tritici blotch is one of the most challenging disease among biotic stress factors. Septoria Leaf Blotch is one of the most important fungal disease in Turkey and the world, causing man...
Investigating the roles of micrornas in biotic stress responses and functional characterization of a novel ztl-type f-box protein via virus induced gene silencing
Dağdaş, Yasin Fatih; Akkaya, Mahinur S.; Department of Biotechnology (2009)
Barley and wheat are the two most important crop species in Turkey. Molecular studies for increasing crop yield of these species are very important for the economic benefits of Turkey. Powdery mildew and yellow rust are the two main pathogens, infecting barley and wheat, respectively in our country and causing a great amount of yield loss each year. Till now, classical genetics studies were performed in order to develop resistant barley and wheat cultivars, but these studies have not been succesful. Therefo...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
M. C. Baloğlu, “Expression analysis of NAC type transcription factors on wheat seedlings under abiotic stress conditions,” Ph.D. - Doctoral Program, Middle East Technical University, 2011.