Development of rapid dipstick assay for food pathogens, Salmonella, by optimized parameters

Date

2018-10-25

Author

Çam, Dilek
Öktem, Hüseyin Avni

Metadata

Show full item record

Item Usage Stats

460
views

0
downloads

Salmonella is among the very important pathogens threating the human and animal health. Rapid and easy detection of these pathogens is crucial. In this context, antibody (Ab) based lateral flow assays (LFAs) which are simple immunochromatographic point of care test kits were developed by gold nanoparticles (GNPs) as labelling agent for Salmonella detection. For that purpose some critical parameters such as reagent concentrations on the capture zones, conjugate concentrations and ideal membrane type needed for LFAs for whole cell detection were tested for naked eye analysis. Therefore, prepared LFAs were applied to the live and heat inactivated cells when they were used alone or included in different bacterial mixtures. Among the test platforms, membrane 180 (M180) was found as an ideal membrane and 36nm GNPs showed highly good labelling in the developed LFAs. Diluted conjugates and low concentrations of reagents affected the test signal negatively. Salmonella was detected in different bacterial mixtures, selectively in 4-5min. The best recognized species by used Ab were S. enteritidis and S. infantis. 5x10(5)S. typhimurium cells were also determined as a limit of detection of this study with mentioned parameters.

Subject Keywords

Lateral flow assay, Salmonella, Gold nanoparticles, Whole cell detection

URI

https://hdl.handle.net/11511/28397

Journal

Journal of Food Science and Technology

DOI

https://doi.org/10.1007/s13197-018-3467-5

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Development of immunoaffinity based detection platforms for food pathogens Çam, Dilek; Öktem, Hüseyin Avni; Department of Biology (2016) Salmonella and E.coli, food pathogens, are among the very important pathogens threating the health. Rapid and easy detection of these pathogens is crucial. In this context, lateral flow assay (LFA) platform prepared by gold nanoparticles (GNPs) based on antibody for Salmonella and E.coli O157:H7 was developed in the first part of this study. In second part, single chain variable fragments (scFv) and SNAP-Tagged full IgG (fusion protein) of se155-4 antibody specific to Salmonella were genetically produced. T...
Development of nucleic acid coated nanoparticle based lateral flow assay for e.coli detection Bingöl, Doğa; Öktem, Hüseyin Avni; Department of Biotechnology (2019) Foodborne diseases have been a serious issue for all societies since the beginning of humanity. Thousands of people suffer from foodborne diseases (FBD) every year according to WHO. These diseases are important due to their high frequency and the expenditures that they affect community negatively. Both developing and developed countries can be affected from large outbreaks of FBD. Although most strains of Escherichia coli are beneficial for host microbiota, some serotypes can lead to serious foodborne illne...
Reconstruction of the temporal signaling network in Salmonella-infected human cells Budak, Gungor; Ozsoy, Oyku Eren; Aydın Son, Yeşim; Can, Tolga; Tunçbağ, Nurcan (2015-07-20) Salmonella enterica is a bacterial pathogen that usually infects its host through food sources. Translocation of the pathogen proteins into the host cells leads to changes in the signaling mechanism either by activating or inhibiting the host proteins. Given that the bacterial infection modifies the response network of the host, a more coherent view of the underlying biological processes and the signaling networks can be obtained by using a network modeling approach based on the reverse engineering principl...
Development of nucleic acid based lateral flow immunochromatographic test platform for salmonella detection Bulut, Onur; Öktem, Hüseyin Avni; Department of Biology (2014) Foodborne diseases have been a crucial problem for the public health. Various agents transmitted by food cause these diseases. However, Salmonella accounts for most of the cases leading to most of the hospitalization and even death. Therefore, rapid detection of Salmonella is a considerable step in order to improve food safety and minimize outbreaks. Nucleic acid based biosensors are fast, simple, economic, easy-to-use and do not require trained personnel or high-cost equipments when compared to the current...
Isolation and Characterization of Salmonella Bacteriophages DENİZ, Aysu; Soyer, Yeşim; Department of Food Engineering (2022-2-03) Numerous foodborne infections and outbreaks are associated with Salmonella which makes it a challenge in terms of human health and economy. Therefore, reducing the prevalence of Salmonella in food and food processing areas is of great importance. Antibiotics are the substances that are commonly used in various stages of food production in order to fight against Salmonella. However, concerns related with the antibiotic use like antibiotic resistance give rise to pursuit of safer methods to eliminate Salmonel...

Citation Formats

D. Çam and H. A. Öktem, “Development of rapid dipstick assay for food pathogens, Salmonella, by optimized parameters,” Journal of Food Science and Technology, pp. 140–148, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/28397.