Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Development of nucleic acid based lateral flow immunochromatographic test platform for salmonella detection
Download
index.pdf
Date
2014
Author
Bulut, Onur
Metadata
Show full item record
Item Usage Stats
308
views
153
downloads
Cite This
Foodborne diseases have been a crucial problem for the public health. Various agents transmitted by food cause these diseases. However, Salmonella accounts for most of the cases leading to most of the hospitalization and even death. Therefore, rapid detection of Salmonella is a considerable step in order to improve food safety and minimize outbreaks. Nucleic acid based biosensors are fast, simple, economic, easy-to-use and do not require trained personnel or high-cost equipments when compared to the current detection methods such as culture and molecular methods. In this study, a nucleic acid based biosensor called lateral flow immunochromatographic assay was developed as a rapid, sensitive, easy-to-use and economic detection system for Salmonella. vi Accordingly, a synthetic oligonucleotide was designed from invA gene which is found in most Salmonella serotypes. The synthetic oligonucleotide was used as the target in order to establish assay procedure and optimize the parameters including time, concentration and temperature. After optimization studies, real targets which were two different PCR products were applied to the assay as targets. The real target samples were 105 bp and 284 bp amplicons of invA gene. In order to form signal probes, AuNPs were functionalized with thiol-modified ssDNAs which were complementary to one part of the target. Biotinylated ssDNAs which were complementary to other part of the target were immobilized onto nitrocellulose membrane to create the capture probe on the test line. When the target was applied to assay, it firstly hybridized with signal probe and then migrated among the test strip. In the test zone, it was capture by the immobilized probe by forming sandwich-type hybridization which resulted in formation of naked eye detectable dark-red colored band. Lateral flow immunochromatographic assay developed in this study achieved detection of different Salmonella specific DNA sequences and it is a promising device for rapid, accurate, easy-to-use and low-cost detection systems.
Subject Keywords
Salmonella.
,
Biosensors.
,
Foodborne diseases.
,
Salmonella
,
Pathogenic microorganisms.
URI
http://etd.lib.metu.edu.tr/upload/12617949/index.pdf
https://hdl.handle.net/11511/24044
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Generation of aptamer against salmonella serovar enteritidis and development of aptamer-based capillary biosensor
Bayraç, Ceren; Öktem, Hüseyin Avni; Eyidoğan, Füsun İnci; Department of Biotechnology (2014)
Food poisoning became one of the most important diseases that threaten to human health. Among all the food-borne pathogens, Salmonella is the most common cause of food-borne infectious disease in the world. Among over 2500 serovars, Salmonella enterica serovar Enteritidis is a common foodborne pathogen associated with human diseases. Limited and time consuming diagnostic techniques such as culturing method or polymerase chain reaction lead to increase in the demands of new detection methods. Therefore, in t...
Development of immunoaffinity based detection platforms for food pathogens
Çam, Dilek; Öktem, Hüseyin Avni; Department of Biology (2016)
Salmonella and E.coli, food pathogens, are among the very important pathogens threating the health. Rapid and easy detection of these pathogens is crucial. In this context, lateral flow assay (LFA) platform prepared by gold nanoparticles (GNPs) based on antibody for Salmonella and E.coli O157:H7 was developed in the first part of this study. In second part, single chain variable fragments (scFv) and SNAP-Tagged full IgG (fusion protein) of se155-4 antibody specific to Salmonella were genetically produced. T...
Development of nucleic acid coated nanoparticle based lateral flow assay for e.coli detection
Bingöl, Doğa; Öktem, Hüseyin Avni; Department of Biotechnology (2019)
Foodborne diseases have been a serious issue for all societies since the beginning of humanity. Thousands of people suffer from foodborne diseases (FBD) every year according to WHO. These diseases are important due to their high frequency and the expenditures that they affect community negatively. Both developing and developed countries can be affected from large outbreaks of FBD. Although most strains of Escherichia coli are beneficial for host microbiota, some serotypes can lead to serious foodborne illne...
Development of rapid dipstick assay for food pathogens, Salmonella, by optimized parameters
Çam, Dilek; Öktem, Hüseyin Avni (Springer Science and Business Media LLC, 2018-10-25)
Salmonella is among the very important pathogens threating the human and animal health. Rapid and easy detection of these pathogens is crucial. In this context, antibody (Ab) based lateral flow assays (LFAs) which are simple immunochromatographic point of care test kits were developed by gold nanoparticles (GNPs) as labelling agent for Salmonella detection. For that purpose some critical parameters such as reagent concentrations on the capture zones, conjugate concentrations and ideal membrane type needed f...
Virulence and antimicrobial resistance genes transfer mechanism between Salmonella Strains by means of Salmonella Genomic Island 1
Namlı, Şahin; Soyer, Yeşim; Department of Food Engineering (2018)
Salmonella, is responsible for significant part of the food borne diseases around the world and isolated abundantly from the food samples. In recent years, with the increasing trend of multidrug-resistance, Salmonella become an important threat for public health. Drug resistance leads inefficiency of antibiotic treatment and it is seemed as a very important problem worldwide as well for Turkey. Drug resistance acceptance mechanism has not been understood yet especially for Salmonella. Salmonella Genomic Isl...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
O. Bulut, “Development of nucleic acid based lateral flow immunochromatographic test platform for salmonella detection,” M.S. - Master of Science, Middle East Technical University, 2014.