Effect of pectin methyl esterase (PME) and CaCl2 infusion on the cell integrity of fresh-cut and frozen-thawed mangoes: An NMR relaxometry study

Date

2014-12

Author

Kırtıl, Emrah
Öztop, Halil Mecit
Barrett, Diane M.
McCarthy, Michael J.

Metadata

Show full item record

Item Usage Stats

281
views

0
downloads

This study describes the use of Nuclear Magnetic Resonance (NMR) relaxometry to understand the effect of pectin methyl esterase (PME) + CaCl2 infusion under different conditions on cell integrity of fresh-cut and frozen-thawed mangoes. Infusion experiments were performed at: atmospheric pressure, vacuum conditions of 50 kPa and 10 kPa. For NMR relaxometry experiments T-2 (spin-spin relaxation time), T-1 (spin-lattice-relaxation times) and 2D T-1-T-2 experiments were performed. Results showed that, as the severity of the vacuum treatment increased, the relaxation times changed significantly (p < 0.05). The number of compartments observed in 1-D relaxation spectra of fresh and frozen-thawed mangoes changed with different treatments. The changes in relaxation times were explained due to formation of a gel formed by the interaction of pectin and calcium. 2D T-1-T-2 relaxation maps showed that compartmentalization was retained after vacuum treatment even for frozen-thawed samples. The study showed that NMR relaxometry is a useful tool to analyze the cell integrity of mangoes exposed to different treatments.

Subject Keywords

CalCl(2)/pectin methyl esterase infusion, Mangoes, 1D/2D NMR relaxometry, Freezing

URI

https://hdl.handle.net/11511/28605

Journal

Food Research International

DOI

https://doi.org/10.1016/j.foodres.2014.10.006

Collections

Department of Food Engineering, Article

Suggestions

OpenMETU
Core

Effect of silver encapsulation on the local structure of titanosilicate ETS-10 GALIOGLU, Sezin; ZAHMAKIRAN, Mehmet; Kalay, Yunus Eren; Özkar, Saim; Akata, Burcu (2012-09-01) Silver(0) nanoparticles stabilized by titanosilicate (ETS-10) framework were synthesized by following a simple two step procedure involving the incorporation of silver(I) (Ag+) ions into ETS-10 matrix via ion-exchange with extra framework Na+ and K+ cations followed by their reduction with sodium borohydride (NaBH4) in aqueous medium all at room temperature. Silver(0) nanoparticles dispersed in the ETS-10 matrix were collected as gray powders and characterized by using advanced analytical methods including ...
Effect of salinity on antioxidant responses of chickpea seedlings Eyidogan, Fuesun; Oez, Mehmet Tufan (2007-10-01) The changes in the activity of antioxidant enzymes, like superoxide dismutase, ascorbate peroxidase, catalase and glutathione reductase, and growth parameters such as length, fresh and dry weight, proline and H2O2 contents, chlorophyll fluorescence (Fv/Fm), quantum yield of PSII and the rate of lipid peroxidation in terms of malondialdehyde in leaf and root tissues of a chickpea cultivar (Cicer arietinum L. cv. Gokc,e)under salt treatment were investigated. Plants were subjected to 0.1, 0.2 and 0.5 M NaCl t...
Effect of solvent choice on cellulose acetate membrane fabrication by phase inversion and deacetylation by alkaline hydrolysis Tekin, Fatma Seden; Çulfaz Emecen, Pınar Zeynep; Department of Chemical Engineering (2022-8) In this study, the effect of solvent choice on cellulose acetate (CA) membrane morphology and performance was investigated to relate this to the thermodynamics and kinetics of phase inversion. Three different solvent systems were used, which are dimethyl sulfoxide (DMSO), the mixture of DMSO: acetone (DA) and DMSO: acetic acid (DHAc) in the ratio of 1:1. Water was used as non-solvent. Acetone and acetic acid were chosen due to their similar solvent quality for cellulose acetate based on Hansen solubility pa...
Effect of hydrogen ion-exchange capacity on activity of resin catalysts in tert-amyl-ethyl-ether synthesis Boz, N; Doğu, Timur; Murtezaoglu, K; Dogu, G (Elsevier BV, 2004-08-10) Activities of ion-exchange resin catalysts, having different hydrogen exchange capacities ranging between 5.1 and 1.3 meq H+/g, on the etherification reactions of 2M1B (2-methyl-1-butene) and 2M2B (2-methyl-2-butene) with ethanol were experimentally tested in a fixed bed reactor. These catalysts were prepared by the heat treatment of Amberlyst-15 catalysts at 220 degreesC at different durations of time and also by the synthesis of sulfonated styrene divinyl benzene cross-linked resins. Activity of these cat...
Effect of thermal treatment on secondary structure and conformational change of mushroom polyphenol oxidase (PPO) as food quality related enzyme: A FTIR study Baltacioglu, Hande; Bayındırlı, Alev; Severcan, Mete; Severcan, Feride (2015-11-15) In order to understand the conformational changes of polyphenol oxidase (PPO), which is a food quality related enzyme, after thermal treatment, secondary structure changes of the enzyme were analyzed by using Fourier Transform Infrared (FTIR) spectroscopy and compared with the change in enzyme activity in the temperature range of 25-80 degrees C. Fourier self-deconvolution, neural network (NN) and curve-fitting analysis were applied to the amide I band of FTIR spectra for detail analysis of secondary struct...

Citation Formats

E. Kırtıl, H. M. Öztop, D. M. Barrett, and M. J. McCarthy, “Effect of pectin methyl esterase (PME) and CaCl2 infusion on the cell integrity of fresh-cut and frozen-thawed mangoes: An NMR relaxometry study,” Food Research International, pp. 409–416, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/28605.