Lactate and ethanol productions by Rhizopus oryzae ATCC 9363 and activities of related pyruvate branch point enzymes

Buyukkileci, Ali Oguz
Hamamcı, Haluk
Yucel, Meral
In Rhizopus oryzae cultivations, lactate production decreased and ethanol and biomass productions increased as inoculated spore concentration increased. Spore concentrations of 1x10(3) and 1x10(4) spores ml(-1) induced tightly packed clumps, whereas 1x10(5) and 1 X 10(6) spores ml(-1) induced filamentous growth.


Valorization of Şırnak asphaltite and biomass
Helvacı, Veysi; Üner, Deniz; Department of Chemical Engineering (2019)
In this study, the hydrogenation and pyrolysis behavior of Şırnak asphaltite, and the synergetic effect of increasing amount of liquid product obtained from steam distillation of Şırnak asphaltite and biomass and dry reforming were studied. For that purpose, Şırnak asphaltite samples were hydrogenated and pyrolyzed and the products are analyzed. For synergetic effect investigation, steam distillation of Şırnak asphaltite with and without the presence of pepper plant biomass was performed. Results show that ...
Anaerobic acidification of sugar-beet processing wastes: Effect of operational parameters
Alkaya, Emrah; Demirer, Göksel Niyazi (Elsevier BV, 2011-01-01)
The objective of this study was to maximize the hydrolysis and acidification of sugar-beet processing wastewater and beet pulp for volatile fatty acid (VFA) production through acidogenic anaerobic metabolism. Experiments were conducted to determine the optimum operational conditions (HRT, waste-mixing ratio and pH) for effective acidification in daily-fed, continuously mixed anaerobic reactors. For this purpose, reactors were operated at 35 +/- 1 degrees C with different combinations of HRT (2-4 days), wast...
Ionic liquid pretreatment allows utilization of high substrate loadings in enzymatic hydrolysis of biomass to produce ethanol from cotton stalks
Haykir, Nazife Isik; Bakir, Ufuk (Elsevier BV, 2013-11-01)
To enhance the enzymatic conversion of cotton stalks to glucose at high substrate loadings and subsequently to produce ethanol, biomass was subjected to ionic liquid and alkaline pretreatments. Ionic liquid, 1-ethyl-3-methylimidazolium acetate (EMIMAc), which reduced biomass crystallinity, was effective in improving the conversion of cotton stalks to glucose (82-85%) regardless of the substrate loading used during enzymatic hydrolysis. On the other hand, the glucose yield decreased from 67% to 55% with an i...
Thermostability and regulation of Clostridium thermocellum L-lactate dehydrogenase expressed in Escherichia coli
Assa, P; Ozkan, M; Özcengiz, Gülay (2005-01-01)
In this study, L-lactate dehydrogenase (L-LDH) of Clostridium thermocellum previously cloned aid expressed in Escherichia coli FMJ39 was partially purified and characterised. Optimum temperature and pH of the enzyme were found as 50 degrees C and 7.5, respectively. Different concentrations of Mn2+ did not affect the enzyme activity. Addition of 20-30 mM Mg2+, or the other hand, increased the LDH activity by about 10%. Relatively high concentrations of NaCl (2 M), fructose-1,6-diphosphate (FDP, 5 mM), ATP (1...
Ethylene and acetaldehyde production by selective oxidation of ethanol using mesoporous V-MCM-41 catalysts
Gucbilmez, Yesim; Doğu, Timur; Balci, Suna (2006-05-10)
Vanadium-incorporated MCM-41 type catalytic materials, which were synthesized by a direct hydrothermal synthesis procedure, showed very high activity and high selectivity in the production of ethylene from ethanol in an oxidative process. Ethylene selectivity showed a significant increase with an increase in temperature over 300 degrees C, while relatively high acetaldehyde selectivities were observed at lower temperatures. An ethylene yield value of 0.66 obtained at 400 degrees C with an O-2/ethanol feed r...
Citation Formats
A. O. Buyukkileci, H. Hamamcı, and M. Yucel, “Lactate and ethanol productions by Rhizopus oryzae ATCC 9363 and activities of related pyruvate branch point enzymes,” JOURNAL OF BIOSCIENCE AND BIOENGINEERING, pp. 464–466, 2006, Accessed: 00, 2020. [Online]. Available: