Lactate and ethanol productions by Rhizopus oryzae ATCC 9363 and activities of related pyruvate branch point enzymes

Buyukkileci, Ali Oguz
Hamamcı, Haluk
Yucel, Meral
In Rhizopus oryzae cultivations, lactate production decreased and ethanol and biomass productions increased as inoculated spore concentration increased. Spore concentrations of 1x10(3) and 1x10(4) spores ml(-1) induced tightly packed clumps, whereas 1x10(5) and 1 X 10(6) spores ml(-1) induced filamentous growth.


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Assa, P; Ozkan, M; Özcengiz, Gülay (2005-01-01)
In this study, L-lactate dehydrogenase (L-LDH) of Clostridium thermocellum previously cloned aid expressed in Escherichia coli FMJ39 was partially purified and characterised. Optimum temperature and pH of the enzyme were found as 50 degrees C and 7.5, respectively. Different concentrations of Mn2+ did not affect the enzyme activity. Addition of 20-30 mM Mg2+, or the other hand, increased the LDH activity by about 10%. Relatively high concentrations of NaCl (2 M), fructose-1,6-diphosphate (FDP, 5 mM), ATP (1...
Citation Formats
A. O. Buyukkileci, H. Hamamcı, and M. Yucel, “Lactate and ethanol productions by Rhizopus oryzae ATCC 9363 and activities of related pyruvate branch point enzymes,” JOURNAL OF BIOSCIENCE AND BIOENGINEERING, pp. 464–466, 2006, Accessed: 00, 2020. [Online]. Available: