Nuclear targeting peptide-modified, DOX-loaded, PHBV nanoparticles enhance drug efficacy by targeting to Saos-2 cell nuclear membranes

Date

2018-01-01

Author

Sahin, Ayla
Eke, Gozde
Buyuksungur, Arda
Hasırcı, Nesrin
Hasırcı, Vasıf Nejat

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The aim of this study was to target nano sized (266 +/- 25nm diameter) poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) particles carrying Doxorubicin (DOX), an anticancer agent, to human osteosarcoma cells (Saos-2). A nuclear targeting molecule (Nuclear Localization Signal, NLS), a 17 a.a. peptide, was attached onto the doxorubicin loaded nanoparticles. NLS conjugated nanoparticles surrounded the cell nuclei, but did not penetrate them. Free doxorubicinand doxorubicin loadednanoparticles entered the cytoplasm and were evenly distributed within the cytoplasm. The localization of the NLS-targetedparticles around the nuclear membrane caused a significantly higher decrease in the cancer cell numbers due to apoptosis or necrosis than the untargeted and free doxorubicin formulations showing the importance of targeting the nanoparticles to the nuclear membrane in the treatment of cancer.

Subject Keywords

PHBV, Biodegradable nanoparticles, Nuclear drug delivery, Doxorubicin, Nuclear localization signal

URI

https://hdl.handle.net/11511/29861

Journal

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION

DOI

https://doi.org/10.1080/09205063.2018.1423812

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

A. Sahin, G. Eke, A. Buyuksungur, N. Hasırcı, and V. N. Hasırcı, “Nuclear targeting peptide-modified, DOX-loaded, PHBV nanoparticles enhance drug efficacy by targeting to Saos-2 cell nuclear membranes,” JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, pp. 507–519, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/29861.