A bilayer scaffold prepared from collagen and carboxymethyl cellulose for skin tissue engineering applications

Bektas, Cemile Kilic
Kimiz, Ilgin
Hasırcı, Vasıf Nejat
Hasırcı, Nesrin
Treatment of chronic skin wound such as diabetic ulcers, burns, pressure wounds are challenging problems in the medical area. The aim of this study was to design a bilayer skin equivalent mimicking the natural one to be used as a tissue engineered skin graft for use in the treatments of problematic wounds, and also as a model to be used in research related to skin, such as determination of the efficacy of transdermal bioactive agents on skin cells and treatment of acute skin damages that require immediate response. In this study, the top two layers of the skin were mimicked by producing a multilayer construct combining two different porous polymeric scaffolds: as the dermis layer a sodium carboxymethyl cellulose (NaCMC) hydrogel on which fibroblasts were added, and as the epidermis layer collagen (Coll) or chondroitin sulfate-incorporated collagen (CollCS) on which keratinocytes were added. The bilayer construct was designed to allow cross-talk between the two cell populations in the subsequent layers and achieves paracrine signalling. It had interconnected porosity, high water content, appropriate stability and elastic moduli. Expression of vascular endothelial growth factor (VEGF), basic-fibroblast growth factor (bFGF) and Interleukin 8 (IL-8), and the production of collagen I, collagen III, laminin and transglutaminase supported the attachment and proliferation of cells on both layers of the construct. Attachment and proliferation of fibroblasts on NaCMC were lower compared to performance of keratinocyte on collagen where keratinocytes created a dense and a stratified layer similar to epidermis. The resulting constructs succesfully mimicked in vitro the natural skin tissue. They are promising as grafts for use in the treatment of deep wounds and also as models for the study of the efficacy of bioactive agents on the skin.


Development and characterization of a tissue engineered multicomponent skin substitute and a skin model
Eke, Gözde; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin; Department of Biotechnology (2018)
The tissue engineered human skin substitute has the potential to fill large areas of skin loss caused by severe burns or chronic wounds. It can also serve as an alternative skin model to in vivo testing of drugs or cosmetic products. The aim was to construct a tissue engineered full-thickness human skin model mimicking the native tissue. To this end, this model was developed as epidermis, dermis and subcutaneous tissue of the skin. The feasibility of the dermis layer was tested by co-culturing fibroblasts a...
Polymeric materials in wound healing
Ulubayram, K; Hasırcı, Nesrin (1997-09-17)
There is a high need for rapid healing in the treatment of severe burns, trauma, diabetic, decubitus ulcers and other conditions where a great damage of the tissue is exist. In those cases, wound should be covered with a dressing which replaces the functions of the natural skin by protecting the loss of body fluid and proteins, preventing bacterial invasion, improving and stimulating the healing process by providing a support for the proliferating cells
EGF containing gelatin-based wound dressings
Ulubayram, K; Cakar, AN; Korkusuz, P; Ertan, C; Hasırcı, Nesrin (2001-06-01)
In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus and venous stasis ulcers, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible to activate the cell proliferation and stimulate the healing process. In this study, synthesis of a novel polymeric bilayer wound dressing containing epidermal growth-factor (EGF)-loaded microspheres was aimed. For this purpose, a natural, nontoxic and biocompatible material, gelatin, w...
Development of a novel zebrafish xenograft model in ache mutants using liver cancer cell lines
Avci, M. Ender; Keskus, Ayse Gokce; Targen, Seniye; Isilak, M. Efe; Ozturk, Mehmet; Atalay, Rengül; ADAMS, MİCHELLE; KONU KARAKAYALI, ÖZLEN (2018-01-25)
Acetylcholinesterase (AChE), an enzyme responsible for degradation of acetylcholine, has been identified as a prognostic marker in liver cancer. Although in vivo Ache tumorigenicity assays in mouse are present, no established liver cancer xenograft model in zebrafish using an ache mutant background exists. Herein, we developed an embryonic zebrafish xenograft model using epithelial (Hep3B) and mesenchymal (SKHep1) liver cancer cell lines in wild-type and achesb55 sibling mutant larvae after characterization...
Analysis of the effects of vancomycin containing bioceramic/polymer composites on biofilm prevention, biocompatibility and osteogenic modification of mesenchymal stem cells
Kankılıç, Berna; Bayramlı, Erdal; Korkusuz, Feza; Department of Biotechnology (2015)
Osteomyelitis is a difficult to treat bone infection. In clinic, osteomyelitis is treated with surgical debridement followed by the antibiotic therapy. But, due to the low blood circulation in the infection area and the production of an exopolysaccharide, biofilm, by the pathogen violence the treatment. Therefore local drug delivery systems are generated. These systems transmit the effective dose to the infection area without any toxic effects. In this study, vancomycin containing PDLLA/β-TCP and PLGA/β-TCP...
Citation Formats
C. K. Bektas, I. Kimiz, A. ŞENDEMİR, V. N. Hasırcı, and N. Hasırcı, “A bilayer scaffold prepared from collagen and carboxymethyl cellulose for skin tissue engineering applications,” JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, pp. 1764–1784, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/29967.