EGF containing gelatin-based wound dressings

Ulubayram, K
Cakar, AN
Korkusuz, P
Ertan, C
Hasırcı, Nesrin
In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus and venous stasis ulcers, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible to activate the cell proliferation and stimulate the healing process. In this study, synthesis of a novel polymeric bilayer wound dressing containing epidermal growth-factor (EGF)-loaded microspheres was aimed. For this purpose, a natural, nontoxic and biocompatible material, gelatin, was chosen as the underlying layer and various porous matrices in sponge form were prepared from gelatin by freeze-drying technique. As the external layer, elastomeric polyurethane membranes were used. Two different doses of EGF was added into the prepared gelatin sponges (1 and 15 mug/cm(2)) to activate cell proliferation. EGF addition was carried out either in free form or within microspheres to achieve prolonged release of EGF for higher efficiency. The prepared systems were tested in in vivo experiments on full-thickness skin defects created on rabbits. At certain intervals, wound areas were measured and tissues from wound areas were biopsied and processed for histological examinations. The wound areas decreased upon low-dose EGF application but the difference between the affects of free EGF and microsphere loaded EGF was not so distinct. Upon increasing the dose of EGF by a factor of 15, it was observed that controlled release of EGF from microspheres provided a higher degree of reduction in the wound areas. Histological investigations showed that the prepared dressings were biocompatible and did not cause any mononuclear cell infiltration or foreign body reaction. The structure of the newly formed dermis was almost the same as that of the normal skin.


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In recent years, functional dressings that can protect the wound area from dehydration, support healing (interactive), and protect the wound against infections have gained importance instead of dry and passive dressings. In this study, it was aimed to develop temporary xanthan gelatin (XGH) and keratin xanthan gelatin hydrogels (KXGHs) that have high exudate absorption capacity, oxygen permeability, and applicability as a wound dressing for medical applications. Firstly, xanthan gelatin hydrogels were produ...
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Recently, functional dressings that can protect the wound area from dehydration and bacterial infection and support healing have gained importance in place of passive dressings. This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC). Firstly, xanthan/gelatin hydrogels were produced by crosslinking with different glycero...
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There is a high need for rapid healing in the treatment of severe burns, trauma, diabetic, decubitus ulcers and other conditions where a great damage of the tissue is exist. In those cases, wound should be covered with a dressing which replaces the functions of the natural skin by protecting the loss of body fluid and proteins, preventing bacterial invasion, improving and stimulating the healing process by providing a support for the proliferating cells
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Malikmammadov, Elbay; Hasırcı, Nesrin; Endoğan Tanır, Tuğba; Department of Micro and Nanotechnology (2017)
Scaffolds produced for tissue engineering applications are promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, fibrous poly(ε-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of β-tricalcium phosphate (β-TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial activity of CS was investi...
Citation Formats
K. Ulubayram, A. Cakar, P. Korkusuz, C. Ertan, and N. Hasırcı, “EGF containing gelatin-based wound dressings,” BIOMATERIALS, pp. 1345–1356, 2001, Accessed: 00, 2020. [Online]. Available: