Biohydrogen production by Rhodobacter capsulatus on acetate at fluctuating temperatures

2010-03-01
Ozgur, Ebru
Uyar, Basar
Ozturk, Yavuz
Yucel, Meral
Gündüz, Ufuk
Eroglu, Inci
Hydrogen is a clean energy alternative to fossil fuels. Photosynthetic bacteria produce hydrogen from organic compounds under anaerobic, nitrogen-limiting conditions through a light-dependent electron transfer process. In this study, the hydrogen production efficiency of phototrophic bacteria, Rhodobacter capsulatus and its Hup mutant strain (an uptake hydrogenase deleted strain) were tested on different initial acetate concentrations at fluctuating temperatures with indoor and outdoor photobioreactors. Acetate was effectively metabolized and H(2) was produced at a high rate. Increasing the initial acetate concentration resulted in a shift in the utilization kinetics of acetate from first order to second order. The effects of fluctuating temperature and day/night cycles on hydrogen production were also studied in indoor and outdoor conditions using acetate as the carbon source. Temperature fluctuations and day/night cycles significantly decreased hydrogen production. It was found that the Hup mutant strain of R. capsulatus has better hydrogen productivity than the wild type parent in outdoor conditions.
RESOURCES CONSERVATION AND RECYCLING

Suggestions

Biohydrogen production from beet molasses by sequential dark and photofermentation
Ozgur, Ebru; Mars, Astrid E.; Peksel, Beguem; Louwerse, Annemarie; Yucel, Meral; Gündüz, Ufuk; Claassen, Pieternel A. M.; Eroglu, Inci (2010-01-01)
Biological hydrogen production using renewable resources is a promising possibility to generate hydrogen in a sustainable way. In this study, a sequential dark and photofermentation has been employed for biohydrogen production using sugar beet molasses as a feedstock. An extreme thermophile Caldicellulosiruptor saccharolyticus was used for the dark fermentation, and several photosynthetic bacteria (Rhodobacter capsulatus wild type, R. capsulatus hup(-) mutant, and Rhodopseudomonas palustris) were used for t...
Product Analysis for Photobiological Production of Hydrogen
Tarlan, Etkin; Koku, Harun; Bayramoğlu, Tuba Hande; Department of Chemical Engineering (2022-8)
Hydrogen (H2) energy has an important place in terms of both reducing environmental pollution problems and having the highest energy content compared to fossil fuels. There are many ways to produce H2, and photofermentation, which is one of the subheadings of biological H2 production, was used in this thesis. Two different strains of Rhodobacter capsulatus strains of photosynthetic bacteria were used: R. capsulatus wild (WT) and uptake hydrogenase deficient mutant YO3 (hup-). In addition to the H2 productio...
Biohydrogen production in an outdoor panel photobioreactor on dark fermentation effluent of molasses
Avcioglu, Sevler Gokce; Ozgur, Ebru; Eroglu, Inci; Yucel, Meral; Gündüz, Ufuk (2011-08-01)
Hydrogen is regarded as an ideal energy carrier if it is produced from renewable resources such as biomass. Sequential operation of dark and photofermentation allows a highly efficient production of hydrogen from biomass, as maximal conversion of the energy in the carbohydrates to hydrogen can be achieved. In this study photofermentative hydrogen production was carried out in a solar panel photobioreactor by Rhodobacter capsulatus wild type (DSM 1710) and Rhodobacter capsulatus hup(-) (YO3) strain on the mo...
Photofermentative hydrogen production from molasses in tubular photobioreactor with pH control
Oflaz, Fatma Betül; Koku, Harun; Department of Chemical Engineering (2019)
Biological hydrogen production has the potential to supply hydrogen from various wastes as feedstock and operation under ambient conditions. In order to obtain cost effective production, photobioreactors (PBRs) that can operate for long durations while utilizing waste are necessary. Two primary issues limiting the duration are decrease in pH and the non-optimal C/N ratio. The main aim of this study was to construct and operate a pH control system for a pilot scale photobioreactor (20 L) to achieve prolonged...
Development of solid sodium borohydride hydrogen gas generator for portable pem fuel cell applications
Boran, Aslı; Eroğlu, İnci; Department of Chemical Engineering (2018)
Hydrogen is considered as a promising candidate to replace fossil fuels. For implementing a hydrogen based infrastructure, hydrogen storage is the mainobstacle that is needed to be overcome. Being boron based compound, sodium borohydride, NaBH4, is a convenient hydrogen storage material for applications like unmanned air vehicles. There are several issues behind commercialization of NaBH4 hydrolysis systems. This doctorate thesis aims to be solution of NaBH4 hydrolysis system by highlighting three main cont...
Citation Formats
E. Ozgur, B. Uyar, Y. Ozturk, M. Yucel, U. Gündüz, and I. Eroglu, “Biohydrogen production by Rhodobacter capsulatus on acetate at fluctuating temperatures,” RESOURCES CONSERVATION AND RECYCLING, pp. 310–314, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30034.