Biohydrogen production by Rhodobacter capsulatus on acetate at fluctuating temperatures

Ozgur, Ebru
Uyar, Basar
Ozturk, Yavuz
Yucel, Meral
Gündüz, Ufuk
Eroglu, Inci
Hydrogen is a clean energy alternative to fossil fuels. Photosynthetic bacteria produce hydrogen from organic compounds under anaerobic, nitrogen-limiting conditions through a light-dependent electron transfer process. In this study, the hydrogen production efficiency of phototrophic bacteria, Rhodobacter capsulatus and its Hup mutant strain (an uptake hydrogenase deleted strain) were tested on different initial acetate concentrations at fluctuating temperatures with indoor and outdoor photobioreactors. Acetate was effectively metabolized and H(2) was produced at a high rate. Increasing the initial acetate concentration resulted in a shift in the utilization kinetics of acetate from first order to second order. The effects of fluctuating temperature and day/night cycles on hydrogen production were also studied in indoor and outdoor conditions using acetate as the carbon source. Temperature fluctuations and day/night cycles significantly decreased hydrogen production. It was found that the Hup mutant strain of R. capsulatus has better hydrogen productivity than the wild type parent in outdoor conditions.


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Hydrogen is considered as a promising candidate to replace fossil fuels. For implementing a hydrogen based infrastructure, hydrogen storage is the mainobstacle that is needed to be overcome. Being boron based compound, sodium borohydride, NaBH4, is a convenient hydrogen storage material for applications like unmanned air vehicles. There are several issues behind commercialization of NaBH4 hydrolysis systems. This doctorate thesis aims to be solution of NaBH4 hydrolysis system by highlighting three main cont...
Citation Formats
E. Ozgur, B. Uyar, Y. Ozturk, M. Yucel, U. Gündüz, and I. Eroglu, “Biohydrogen production by Rhodobacter capsulatus on acetate at fluctuating temperatures,” RESOURCES CONSERVATION AND RECYCLING, pp. 310–314, 2010, Accessed: 00, 2020. [Online]. Available: