Biohydrogen production from beet molasses by sequential dark and photofermentation

Ozgur, Ebru
Mars, Astrid E.
Peksel, Beguem
Louwerse, Annemarie
Yucel, Meral
Gündüz, Ufuk
Claassen, Pieternel A. M.
Eroglu, Inci
Biological hydrogen production using renewable resources is a promising possibility to generate hydrogen in a sustainable way. In this study, a sequential dark and photofermentation has been employed for biohydrogen production using sugar beet molasses as a feedstock. An extreme thermophile Caldicellulosiruptor saccharolyticus was used for the dark fermentation, and several photosynthetic bacteria (Rhodobacter capsulatus wild type, R. capsulatus hup(-) mutant, and Rhodopseudomonas palustris) were used for the photofermentation. C. saccharolyticus was grown in a pH-controlled bioreactor, in batch mode, on molasses with an initial sucrose concentration of 15 g/L. The influence of additions of NH4+ and yeast extract on sucrose consumption and hydrogen production was determined. The highest hydrogen yield (4.2 mol of H-2/mol sucrose) and maximum volumetric productivity (7.1 mmol H-2/L-c.h) were obtained in the absence of NH4+. The effluent of the dark fermentation containing no NH4+ was fed to a photobioreactor, and hydrogen production was monitored under continuous illumination, in batch mode. Productivity and yield were improved by dilution of the dark fermentor effluent (DFE) and the additions of buffer, iron-citrate and sodium molybdate. The highest hydrogen yield (58% of the theoretical hydrogen yield of the consumed organic acids) and productivity (1.37 mmol H-2/L-c.h) were attained using the hup(-) mutant of R. capsulatus. The overall hydrogen yield from sucrose increased from the maximum of 4.2 mol H-2/mol sucrose in dark fermentation to 13.7 mol H-2/mol sucrose (corresponding to 57% of the theoretical yield of 24 mol of H-2/mole of sucrose) by sequential dark and photofermentation. (C) 2009 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.


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Citation Formats
E. Ozgur et al., “Biohydrogen production from beet molasses by sequential dark and photofermentation,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 511–517, 2010, Accessed: 00, 2020. [Online]. Available: