Enzymatic Hydrolysis of Fruit Peels and Other Lignocellulosic Biomass as a Source of Sugar

POCAN, Pelin
Öztop, Halil Mecit
Hamamcı, Haluk
Glucose and reducing sugars were obtained from corn cobs, orange and pomegranate peels by employing different enzymatic hydrolysis and pretreatment strategies. For the hydrolysis of corn cobs, effect of alkaline pretreatment was examined. Effect of cellobiase loading on hydrolysis was also investigated. Glucose content was measured using a Blood Glucose Monitor. When cellobiase was also added to hydrolysate, glucose and TRS yield increased from 20 and 34% respectively. Unlike corncobs, alkaline pretreatment was not applied to pomegranate and orange peels due to low lignin content. For the hydrolysis of these substrates, a pectic enzyme mixture was also used. Reasonable glucose and total reducing sugar yield from pomegranate and orange peel hydrolysis was achieved without any pretreatment. It was shown that while efficient corn cob hydrolysis needed synergistic effect of cellulase and beta-glucosidase combination, fruit peels hydrolysis needed synergistic effect of cellulase, beta-glucosidase and pectinase.


Enzymatic hydrolysis of fruit peels and other lignocellulosic biomass as a source of sugar for fermentation
Poçan, Pelin; Öztop, Halil Mecit; Hamamcı, Haluk; Department of Food Engineering (2015)
In this study, enzymatic hydrolysis of corn cobs, orange and pomegranate peels were evaluated. For the hydrolysis of corn cobs, effect of alkaline pretreatment was examined and it was found that glucose and reducing sugar yield (%) for the pretreated corn cobs increased from 7% to 21.5% and 14% to 33.6% respectively. Effect of cellobiase loading on hydrolysis efficiency was also investigated. It was observed that when cellobiase was added to hydrolysate in addition to cellulase, glucose and total reducing s...
Enzymatic peeling of apricots nectarines and peaches
TOKER, İlkem; Bayındırlı, Alev (2003-03-01)
Different combinations of Peelzym (I, II, III and IV) concentration (0.1, 0.55, 1 cm(3) enzyme/100 cm(3) solution), temperature (20degreesC, 35degreesC, 50degreesC), pH (3.0, 4.5, 6.0) and time (20, 40, 60 min) were used for enzymatic peeling of apricots, nectarines and peaches. The optimum conditions were determined by using response surface methodology. Peeling yield, absorption of enzyme solution and texture changes were measured after peeling. For peeling of apricots with Peelzym IV, the optimum tempera...
Optimising clarification of carrot juice by bacterial crude pectinase
Uzuner, Sibel; Çekmecelioğlu, Deniz (2015-12-01)
This study was undertaken to search for potential use of crude bacterial pectinase enzyme produced from Bacillus subtilis grown on hazelnut shell hydrolysate in clarification of carrot juice and to optimize the enzyme load, pH and time using the Box-Behnken response surface methodology (RSM). The carrot juice was treated with the crude pectinase enzyme (5.60 U mL(-1)) at different concentrations (0.1-0.5%), pH (4-7), and time (2-6 h). The obtained enzyme was also compared with commercial fungal pectinase at...
Recombinant protein production by sucrose-utilizing Escherichia coli W: untreated beet molasses-based feeding strategy development
Akdag, Burcu; Çalık, Pınar (2015-06-01)
BACKGROUNDUntreated beet molasses feeding strategy was developed for recombinant thermostable glucose isomerase (GI) production by sucrose-utilizing Escherichia coli W.
Biosynthetic Route for the Preparation of Nonregular Gold Nanoparticles Using Aqueous Extracted of Nettle (Urtica dioica L.) Plant
Molaei, Rahim; Farhadi, Khalil; Forough, Mehrdad; Pourhossein, Atefeh (2015-01-01)
In this study, the bioreduction of Au (III) ions to Au-0 are proposed by using the biomass come from nettle (Urtica dioica L.) leaves as a pharmaceutical plant. This biomimetic, safe and cost effective procedure has led to the preparation of irregular gold nanosized particles. The synthesized gold nanoparticles were characterized with UV-Vis, transmission electron microscopy, X-ray diffraction, dynamic light scattering, FT-IR, and zeta potential measurement techniques. The obtained results indicated the for...
Citation Formats
P. POCAN, E. BAHÇEGÜL, H. M. Öztop, and H. Hamamcı, “Enzymatic Hydrolysis of Fruit Peels and Other Lignocellulosic Biomass as a Source of Sugar,” WASTE AND BIOMASS VALORIZATION, pp. 929–937, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30234.