Enzymatic hydrolysis of fruit peels and other lignocellulosic biomass as a source of sugar for fermentation

Poçan, Pelin
In this study, enzymatic hydrolysis of corn cobs, orange and pomegranate peels were evaluated. For the hydrolysis of corn cobs, effect of alkaline pretreatment was examined and it was found that glucose and reducing sugar yield (%) for the pretreated corn cobs increased from 7% to 21.5% and 14% to 33.6% respectively. Effect of cellobiase loading on hydrolysis efficiency was also investigated. It was observed that when cellobiase was added to hydrolysate in addition to cellulase, glucose and total reducing sugar yield increased from 11% to 20% and 21% to 33.5% respectively. On the other hand, although cellulase amount was increased successively while cellobiase was kept at constant load, glucose and total reducing sugar yield did not change and was constant at around 20% and 35% respectively. Due to their either low lignin content or high sugar content, alkaline pretreatment was not preferred for the pomegranate and orange peels. For the hydrolysis of these substrates, in addition to cellulolytic enzymes pectic enzymes were also used. It was found that when pectinase was used in addition to cellulase, conversion to glucose and total reducing sugar (%) increased significantly.


Enzymatic Hydrolysis of Fruit Peels and Other Lignocellulosic Biomass as a Source of Sugar
POCAN, Pelin; BAHÇEGÜL, Erinc; Öztop, Halil Mecit; Hamamcı, Haluk (2018-06-01)
Glucose and reducing sugars were obtained from corn cobs, orange and pomegranate peels by employing different enzymatic hydrolysis and pretreatment strategies. For the hydrolysis of corn cobs, effect of alkaline pretreatment was examined. Effect of cellobiase loading on hydrolysis was also investigated. Glucose content was measured using a Blood Glucose Monitor. When cellobiase was also added to hydrolysate, glucose and TRS yield increased from 20 and 34% respectively. Unlike corncobs, alkaline pretreatment...
KAVAS, MUSA; Akca, Oya Ercan; Akcay, Ufuk Celikkol; Peksel, Begum; Eroğlu, Seçkin; Öktem, Hüseyin Avni; Yucel, Meral (2015-01-01)
In this study, the effects of long-term NaCl treatment were investigated in two cultivars of peanut designated as drought-resistant and drought-sensitive. Growth parameters, changes in the concentrations of MDA, H2O2 and proline, and the activities of antioxidant enzymes were determined under salinity stress. Growth parameters indicated the superiority of cv. Florispan to cv. Gazipasa under milder salinity stress treatment. However, comparative analysis of the two cultivars showed that MDA, H2O2, ion leakag...
Optimising clarification of carrot juice by bacterial crude pectinase
Uzuner, Sibel; Çekmecelioğlu, Deniz (2015-12-01)
This study was undertaken to search for potential use of crude bacterial pectinase enzyme produced from Bacillus subtilis grown on hazelnut shell hydrolysate in clarification of carrot juice and to optimize the enzyme load, pH and time using the Box-Behnken response surface methodology (RSM). The carrot juice was treated with the crude pectinase enzyme (5.60 U mL(-1)) at different concentrations (0.1-0.5%), pH (4-7), and time (2-6 h). The obtained enzyme was also compared with commercial fungal pectinase at...
Kazanç Özerinç, Feyza (2019-09-01)
This study investigates the particulate matter formation during combustion of olive residue, almond shell, and Tunçbilek lignite. Selected fuels (olive residue and Tunçbilek lignite) were also co-fired to evaluate the influence on particulate matter emission. Olive residue was ground to different size ranges (< 125 µm and 212–300 µm) to investigate the influence of the particle size and blended in different ratios of biomass / coal to analyse the interactions between fuels. Tests were performed in a drop tu...
Influences of Microemulsion Cross-linking Reaction and Ball-milling on Particle Size Characteristics of Potato and Maize Starches
Zhou, Yu-guang; Li, Dong; Wang, Li-jun; Özkan, Necati; Chen, Xiao Dong; MAO, Zhi-huai (2006-01-01)
In this study, microemulsion cross-linking treatment was used on food grade potato and maize starches for preparing micro starch particles. Laser diffraction technique was introduced to measure the particle size characteristics, including the median particle diameter (d (50)), surface area mean diameter D [3, 2], volume mean diameter D [4, 3] and specific surface area of micro potato and maize starch particles. The volume distributions and number distributions were also analyzed using Mastersizer 2000 Softw...
Citation Formats
P. Poçan, “Enzymatic hydrolysis of fruit peels and other lignocellulosic biomass as a source of sugar for fermentation,” M.S. - Master of Science, Middle East Technical University, 2015.