Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Influence of keratocytes and retinal pigment epithelial cells on the mechanical properties of polyester-based tissue engineering micropatterned films
Date
2007-08-01
Author
Zorlutuna, Pinar
Builles, Nicolas
Damour, Odile
Elsheikh, Ahmed
Hasırcı, Vasıf Nejat
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
214
views
0
downloads
Cite This
In this paper the mechanical properties of micropatterned polyester films prepared to serve as tissue engineering scaffolds of cornea were examined. Films were prepared by solvent casting of blends of poly(L-lactide-co-D,L-lactide) and poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid), on a micropatterned silicon template. They were seeded with keratocytes or retinal pigment epithelia] cells and subjected to tensile testing to assess the contribution of cells and the deposited extra-cellular matrix (ECM) to the mechanical properties of the scaffold. In all the tests. the films used were wet and the cells were not fixed. Cell-free scaffolds showed a gradual deterioration in strength upon incubation in the cell culture medium at 37 degrees C; the deterioration rate was highest in the first week and decreased significantly over the second and third weeks. The ultimate strength of the cell-free scaffolds decreased from 0.99 to 0.42 N/mm after 21 days of incubation. Cell seeded scaffolds showed a more complicated mechanical strength profile. Their response was found to depend both on the extent of surface coverage and on the cell type. The results were examined after dividing the data into two groups of lower and higher stiffness. For keratocyte seeded scaffolds, the strength of the high stiffness groups continued to increase as the incubation period increased while the lower stiffness groups did not show a distinct change. For the keratocyte seeded scaffolds, tensile strength increased from 0.65 N/mm on Day 7 to 0.73 N/mm on Day 21. On the other hand, the scaffolds seeded with retinal pigment epithelial cells showed a gradual deterioration over time in both the higher and lower stiffness groups. For epithelial cell seeded scaffolds this was 0.98 N/mm on Day 7 and decreased to 0.77 N/mm on Day 21 still an improvement over the unseeded scaffolds. This most probably was a result of a lower rate of ECM secretion in comparison to keratocytes and the newly secreted ECM could not compensate for the influence of scaffold degradation on the mechanical properties. It could, therefore, be concluded that cell seeding plays a positive role in strengthening a tissue engineered construct, and cell type has a significant influence on the extent of this improvement.
Subject Keywords
Cornea
,
Mechanical properties
,
Tissue engineering
,
Polyester
URI
https://hdl.handle.net/11511/30355
Journal
BIOMATERIALS
DOI
https://doi.org/10.1016/j.biomaterials.2007.04.013
Collections
Graduate School of Natural and Applied Sciences, Article
Suggestions
OpenMETU
Core
Effect of human corneal keratocytes and retinal pigment epithelial cells on the mechanical properties of micropatterned collagen films
Vrana, Nihal E.; Elsheikh, Ahmed; Builles, Nicolas; Damour, Odile; Hasırcı, Vasıf Nejat (2007-10-01)
Collagen-based micropatterned films were seeded with human corneal keratocyte and epithelial cells to study their mechanical properties as tissue engineering substrates. The patterns were in the form of parallel channels with slanted walls. Influence of cell presence, type and growth on the mechanical properties of the films was investigated. Unseeded films showed gradual strength reduction from an initial value of 0.046 N/mm, possibly due to degradation, down to 0.032 +/- 0.012 N/mm in 2 weeks. Keratocyte ...
Free standing layer-by-layer films of polyethyleneimine and poly(l-lysine) for potential use in corneal stroma engineering
Altay, Gizem; Hasırcı, Vasıf Nejat; Khademhosseini, Ali; Department of Biomedical Engineering (2011)
In this study we fabricated free standing multilayer films of polyelectrolyte complexes for potential use in tissue engineering of corneal stroma by using the layer-by-layer (LbL) approach. In the formation of these LbL films negatively charged, photocrosslinkable (methacrylated) hyaluronic acid (MA-HA) was used along with polycations polyethyleneimine (PEI) and poly(L-lysine) (PLL). Type I collagen (Col) was blended in with PLL for improving the water absorption and cell attachment properties of the films....
Transient dynamic response of viscoelastic cylinders enclosed in filament wound cylindrical composites
Şen, Özge; Turhan, Doğan; Department of Engineering Sciences (2005)
In this study, transient dynamic response of viscoelastic cylinders enclosed in filament wound cylindrical composites is investigated. Thermal effects, in addition to mechanical effects, are taken into consideration. A generalized thermoelasticity theory which incorporates the temperature rate among the constitutive variables and is referred to as temperature-rate dependent thermoelasticity theory is employed. This theory predicts finite heat propagation speeds. The body considered in this thesis consists o...
Behaviour of PLA/POSS nanocomposites: Effects of filler content, functional group and copolymer compatibilization
Meyva Zeybek, Yelda; Kaynak, Cevdet (2021-04-01)
The main purpose of this study was to investigate influences of three parameters on the mechanical and thermal properties of the polylactide (PLA) matrix nanocomposites filled with polyhedral oligomeric silsesquioxane (POSS) particles. For the first parameter of "Filler Content", nanocomposites with 1, 3, 5, 7 wt% basic POSS structure were compared. For the second parameter of "Functional Group," basic POSS structure having only nonpolar isobutyl groups were compared with three other functionalized POSS str...
Cornea engineering on polyester carriers
Zorlutuna, P.; Tezcaner, Ayşen; Kiyat, I.; Aydinli, A.; Hasırcı, Vasıf Nejat (2006-10-01)
In this study, biodegradable polyester based carriers were designed for tissue engineering of the epithelial and the stromal layers of the cornea, and the final construct was tested in vitro. In the construction of the epithelial layer, micropatterned films were prepared from blends of biodegradable and biocompatible polyesters of natural (PHBV) and synthetic (P(L/DL)LA) origin, and these films were seeded with D407 (retinal pigment epithelial) cells. To improve cell adhesion and growth, the films were coat...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
P. Zorlutuna, N. Builles, O. Damour, A. Elsheikh, and V. N. Hasırcı, “Influence of keratocytes and retinal pigment epithelial cells on the mechanical properties of polyester-based tissue engineering micropatterned films,”
BIOMATERIALS
, pp. 3489–3496, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30355.