Evaluation of neointimal hyperplasia on tranilast-coated synthetic vascular grafts: An experimental study

Date

2007-01-01

Author

Karakayali, Feza
Haberal, Nihan
Tufan, Hale
Hasırcı, Nesrin
BaSaran, Ozgur
Sevmis, Sinasi
Akdur, Aydin
Kızıltay, Aysel
Haberal, Mehmet

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

220
views

0
downloads

Tranilast is an antiallergic drug that interferes with proliferation and migration of vascular smooth muscle cell induced by platelet-derived growth factor (PDGF) and transforming growth factor-beta 1 (TGF-beta 1). We investigated the local effect of tranilast on neointimal hyperplasia using tranilastcoated prosthetic grafts. The inner sides of the thin-walled polytetrafluoroethylene (PTFE) grafts were coated with chitosan and tranilast containing chitosan solution. Wistar albino rats (32) were used in the study. Patches (1 x 2 mm) for vascular grafts were prepared. Three groups were tested: group 1 (n = 12; tranilast coated), group 2 (n = 10; adhesive-only film-layer-coated), and group 3 (n = 10; normal ePTFE patch grafts sutured to the carotid arteries of the rats). Recipient sites of the carotid arteries were excised 4 weeks after surgery. All sections were examined histologically for graft patency, thrombus formation, and neointimal thickness. Expression of PDGF, fibroblast growth factor, and TGF-beta 1 on cross-sections of the neointima were evaluated by immunohistochemistry. No significant differences were found regarding mean neointimal thicknesses. PDGF and TGF-beta-1 expressions were significantly lower in group 1. Although a decrease in local effect of tranilast was observed for growth factor expressions at a drug concentration of 0.05 mg/cm(2), a significant reduction in neointimal hyperplasia was not achieved. The coating concentration of 0.05 mg/cm(2) may have been too low to produce an antiproliferative effect. Given our promising results, further studies are recommended and planned using different drug concentrations and time intervals.

Subject Keywords

Neointimal hyperplasia, Tranilast, Vascular graft

URI

https://hdl.handle.net/11511/30366

Journal

JOURNAL OF INVESTIGATIVE SURGERY

DOI

https://doi.org/10.1080/08941930701365101

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Evaluation of methylation profiles of an epidermal growth factor receptor gene in a head and neck squamous cell carcinoma patient group Mutlu, M.; Mutlu, Pelin; Azarkan, S.; Baylr, Ö.; Öcal, B.; Saylam, G.; KORKMAZ, MEHMET HAKAN (2021-03-23) Upregulation of the epidermal growth factor receptor (EGFR) gene has shown an important impact on the development of head and neck cancers due to its important regulation role on multiple cell signaling pathways. The aim of this study was to investigate the methylation pattern of the promoter region of the EGFR gene between head and neck squamous cell carcinoma (HNSCC) patients and a control group. Forty-seven unrelated HNSCC patients, clinically diagnosed at the Department of Otorhinolaryngology, Dlşkapl Y...
Expression System for Recombinant Human Growth Hormone Production from Bacillus subtilis ÖZDAMAR, HASAN TUNÇER; Sentuerk, Birguel; Yilmaz, Oezge Deniz; Calik, Guezide; Celik, Eda; Çalık, Pınar (2009-01-01) We demonstrate for the first time, an expression system mimicking serine alkaline protease synthesis and secretion, producing native form of human growth hormone (hGH) from Bacillus subtilis. A hybrid-gene of two DNA fragments, i.e., signal (pre-) DNA sequence of B. licheniformis serine alkaline protease gene (subC) and cDNA encoding hGH, were cloned into pMK4 and expressed under deg-promoter in B. subtilis. Recombinant-hGH (rhGH) produced by B. subtilis carrying pMK4::pre(subC)::hGH was secreted. N-termina...
Evaluation of functional changes in akr1b1 and akr1b10 overexpressing colorectal cancer cell lines Güderer, İsmail; Banerjee, Sreeparna; Department of Biology (2021-2-15) Aldo-keto reductases (AKRs) are nicotinamide adenine dinucleotide phosphate (NADPH)-dependent enzymes with diverse cellular metabolism functions. AKR1B1 and AKR1B10 are two of the most studied enzymes in the AKR family. AKR1B1 reduces excess glucose into sorbitol using reducing electrons from NADPH, and the hyperactivation of the AKR1B1 pathways is associated with oxidative stress and cell death. AKR1B10 is a poor reductant of glucose but is a vital enzyme that can metabolize retinol and many other drugs an...
Assessment of 17beta-estradiol-estrogen receptor alpha complex-mediated changes in genome-wide methylation and gene expression profiles User, Sırma Damla; Muyan, Mesut; Department of Biology (2016) 17β-estradiol (E2), the most potent estrogen hormone, induces cellular responses primarily through Estrogen Receptor-alpha (ERα), which is a transcription factor. Interfering E2 signaling indicates that E2 is mitogenic for cells, exemplified by MCF7 cells derived from breast adenocarcinoma, synthesizing ERα endogenously. Studies used exogenous expression of ERα in ERα-negative cell lines to examine structural/functional properties of the receptor. What was unexpected from these studies is the observation th...
Molecular docking study of fda-approved drugs to inhibit the bacterial ribosome Ateş, Beril; Yüce, Merve; Levitaş, Ozge Kurkcuoglu; Sungur, Fethiye Aylin (Orta Doğu Teknik Üniversitesi Enformatik Enstitüsü; 2022-10) Ribosomes are large macromolecular complexes responsible for cellular protein synthesis. It consists of two subunits; called 30S small and 50S large subunits in bacteria, involving antibiotic binding regions. This mac- romolecular machine is one of the significant targets of conventional antibiotics because protein synthesis can be stopped by targeting functional sites in the ribosome. For instance, several antibiotics target the decoding center responsible for deciphering the genetic code, as well as mRNA ...

Citation Formats

F. Karakayali et al., “Evaluation of neointimal hyperplasia on tranilast-coated synthetic vascular grafts: An experimental study,” JOURNAL OF INVESTIGATIVE SURGERY, pp. 167–173, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30366.