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A cost-effective and simple culture method for primary hepatocytes
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Date
2011-03-01
Author
Aday, Sezin
Hasırcı, Nesrin
Deliloglu Gurhan, Ismet
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Hepatocytes, the major epithelial cells of the liver, maintain their morphology in culture dishes coated with extracellular matrix (ECM) components such as collagen and fibronectin or biodegradable polymers (e.g. chitosan, gelatin). In these coated dishes, survival of cells and maintaining of liver-specific functions may increase. The aim of this study was to determine a suitable, cost-effective and simple system for hepatocyte isolation and culture which may be useful for various applications such as in vitro toxicology studies, hepatocyte transplantation and bioartificial liver (BAL) systems. In order to obtain primary cultures, hepatocytes were isolated from liver by an enzymatic method and cultured on plates coated with collagen, chitosan or gelatin. Collagen, gelatin-sandwich and gelatin-cell mixture methods were also evaluated. Morphology and attachment of the cells were observed by inverted microscope and scanning electron microscope (SEM). An MTT assay was used to determine cell viability and mitochondrial activity.
Subject Keywords
Collagen
,
Chitosan
,
Gelatin
,
Enzymatic isolation
,
Primary hepatocytes
URI
https://hdl.handle.net/11511/30373
Journal
ANIMAL CELLS AND SYSTEMS
DOI
https://doi.org/10.1080/19768354.2011.555140
Collections
Graduate School of Natural and Applied Sciences, Article
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S. Aday, N. Hasırcı, and I. Deliloglu Gurhan, “A cost-effective and simple culture method for primary hepatocytes,”
ANIMAL CELLS AND SYSTEMS
, pp. 19–27, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30373.