Construction and in vitro testing of a multilayered, tissue-engineered meniscus

Date

2014-05-01

Author

Bahcecioglu, Gokhan
Buyuksungur, Arda
Kızıltay, Aysel
Hasırcı, Nesrin
Hasırcı, Vasıf Nejat

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

115
views

0
downloads

A novel three-dimensional construct was designed to serve as a substitute for the natural meniscus tissue, and tested in vitro. The design consisted of mats of aligned collagen micro/nanofibers, entrapped within a macroporous poly(l-lactic acid)/poly(lactic acid-co-glycolic acid) foam coated with Ca+2-cross-linked alginic acid. Fibrochondrocytes derived from human meniscus were tested in vitro to study cell attachment and proliferation. After a 21-day culture, the cells populating the constructs were shown to produce extracellular matrix components specific for fibrocartilages, such as collagen Types I and II and aggrecan. Coating the foam with alginate increased the compressive modulus of the collagen-containing constructs (from 320 to 381 kPa, after 21 days of incubation with fibrochondrocytes) but decreased cell attachment and proliferation, as well as aggrecan production. Collagen fibers substantially increased the tensile modulus of the cell-seeded constructs (from 0.98 to 1.71 MPa for uncoated and from 0.67 to 1.32 MPa for coated samples). All constructs produced extracellular matrix components specific for fibrocartilages. These findings indicate that these constructs have potential for use as meniscus substitutes.

Subject Keywords

Meniscus, Multilayered, Aligned collagen fibers, Mechanical properties, Fibrochondrocytes, Micro/nanofibers, Alginic acid, Poly(L-lactic acid), Poly(lactic acid-co-glycolic acid), Human knee-joint, Electrospun scaffolds, Nanofibrous scaffolds, Growth-factors, Collagen, Fibrocartilage, Culture, Fibrochondrocytes, Ultrastructure, Injury

URI

https://hdl.handle.net/11511/30417

Journal

JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS

DOI

https://doi.org/10.1177/0883911514529688

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Fabrication and characterization of bilayered tissue scaffolds incorporating bioactive agents for skin tissue engineering applications Aktürk, Ömer; Keskin, Dilek; Bilici, Temel; Department of Engineering Sciences (2015) In this study, it was aimed to fabricate tissue scaffolds from different biological polymers (collagen, silk fibroin and sericin) for skin tissue engineering applications. For this purpose, bilayered scaffolds composed of epidermal (collagen/sericin films) and dermal (collagen sponges, collagen matrices or silk fibroin matrices) layers were produced with different biomaterial fabrication methods. Casting and solvent evaporation (film), lyophilization/freeze-drying (sponge) and dry/wet electro-spinning (micr...
Preparation and characterization of poly(epsilon-caprolactone) scaffolds modified with cell-loaded fibrin gel Malikmammadov, Elbay; Endoğan Tanır, Tuğba; Kızıltay, Aysel; Hasırcı, Nesrin (2019-03-15) Poly(epsilon-caprolactone) (PCL) is one of the most commonly used polymers in the production of tissue engineered scaffolds for hard tissue treatments. Incorporation of cells into these scaffolds significantly enhances the healing rate of the tissue. In this study, PCL scaffolds were prepared by wet spinning technique and modified by addition of fibrinogen in order to form a fibrin network between the PCL fibers. By this way, scaffolds would have micro and nanofibers in their structures. Drying of the wet s...
A novel construct as a cell carrier for tissue engineering ZORLUTUNA, PINAR; Tezcaner, Ayşen; Hasırcı, Vasıf Nejat (2008-01-01) A 3D scaffold, in the form of a foam, with the top surface carrying a micropattern, was constructed from biodegradable polyesters poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) and poly(L-lactide-co-D, L-lactide) (P(L/DL)LA) to serve as a substitute for the extracellular matrix (ECM) of tissues with more than one cell type. The construct was tested in vitro for engineering of such tissues using fibroblasts (3T3) and epithelial cells ( retinal pigment epithelial cells, D407). The patterned surfa...
Microfibrous scaffolds from poly(L-lactide-co-epsilon-caprolactone) blended with xeno-free collagen/hyaluronic acid for improvement of vascularization in tissue engineering applications KENAR, HALİME; Ozdogan, Candan Yilmaz; Dumlu, Cansu; DOĞER, EMEK; Kose, Gamze Torun; Hasırcı, Vasıf Nejat (2019-04-01) Success of 3D tissue substitutes in clinical applications depends on the presence of vascular networks in their structure. Accordingly, research in tissue engineering is focused on the stimulation of angiogenesis or generation of a vascular network in the scaffolds prior to implantation. A novel, xeno-free, collagen/hyaluronic acid-based poly(L-lactide-co-epsilon-caprolactone) (PLC/COL/HA) (20/9.5/0.5 w/w/w) microfibrous scaffold was produced by electrospinning. Collagen types I and III, and hyaluronic acid...
Preparation of sericin based wound dressing and investigation of its biomaterial properties Aktürk, Ömer; Keskin, Dilek; Department of Engineering Sciences (2009) In this study, it was aimed to produce sericine/collagen composite membranes and to investigate their properties as a wound dressing. Different membrane compositions were prepared by casting and solvent evaporation method. After initial studies for optimization of ratios, membrane groups at two different thicknesses were prepared for each selected ratio and cross-linked with 3 % (w/v) glutaraldehyde (GTA). Considering the wound dressing requirements, equilibrium degree of swelling (EDS), water vapor transmi...

Citation Formats

G. Bahcecioglu, A. Buyuksungur, A. Kızıltay, N. Hasırcı, and V. N. Hasırcı, “Construction and in vitro testing of a multilayered, tissue-engineered meniscus,” JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, pp. 235–253, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30417.