Microfibrous scaffolds from poly(L-lactide-co-epsilon-caprolactone) blended with xeno-free collagen/hyaluronic acid for improvement of vascularization in tissue engineering applications

Date

2019-04-01

Author

KENAR, HALİME
Ozdogan, Candan Yilmaz
Dumlu, Cansu
DOĞER, EMEK
Kose, Gamze Torun
Hasırcı, Vasıf Nejat

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

242
views

0
downloads

Success of 3D tissue substitutes in clinical applications depends on the presence of vascular networks in their structure. Accordingly, research in tissue engineering is focused on the stimulation of angiogenesis or generation of a vascular network in the scaffolds prior to implantation. A novel, xeno-free, collagen/hyaluronic acid-based poly(L-lactide-co-epsilon-caprolactone) (PLC/COL/HA) (20/9.5/0.5 w/w/w) microfibrous scaffold was produced by electrospinning. Collagen types I and III, and hyaluronic acid were isolated from human umbilical cords and blended with the GMP grade PLC. When compared with PLC scaffolds the PLC/COL/HA had higher water uptake capacity (103% vs 66%) which may have contributed to the decrease in its Young's Modulus (from 1.31 to 0.89 MPa). The PLC/COL/HA better supported adipose tissue-derived mesenchymal stem cell (AT MSC) adhesion; within 24 h the cell number on the PLC/COL/HA scaffolds was 3 fold higher. Co-culture of human umbilical vein endothelial cells and AT MSCs induced capillary formation on both scaffold types, but the PLC/COL/HA led to formation of interconnected vessels whose total length was 1.6 fold of the total vessel length on PLC. Clinical use of this scaffold would eliminate the immune response triggered by xenogeneic collagen and transmission of animal-borne diseases while promoting a better vascular network formation.

Subject Keywords

Vascularization, Hyaluronic acid, Collagen, Human umbilical cord, Electrospinning, Xeno-free scaffold

URI

https://hdl.handle.net/11511/32107

Journal

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

DOI

https://doi.org/10.1016/j.msec.2018.12.011

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Marine Algae-PLA composites as de novo alternative to porcine derived collagen membranes Sayin, S.; Kohlhaas, T.; Veziroglu, S.; Okudan, E. S.; Naz, M.; Schroeder, S.; Saygili, E.; Acil, Y.; Faupel, F.; Wiltfang, J.; Aktas, O. C.; Guelses, A. (2020-09-01) Resorbable and volume-stable collagen matrices have been preferred as grafting materials for diverse clinical applications in recent years. On the other hand, there is always an existing risk of transmission of infection with the cells or the tissues of the graft in using such collagen matrices. Moreover, considering the economic aspects, there is also a need for cost-efficient alternative materials. In this sense, marine algae can be considered as alternatives since they represent vast and cheap source of ...
Micropatterned Surfaces Expose the Coupling between Actin Cytoskeleton-Lamin/Nesprin and Nuclear Deformability of Breast Cancer Cells with Different Malignancies. Antmen Altunsoy, Ezgi; Hasirci, Vasif (2021-01-01) Mechanotransduction proteins transfer mechanical stimuli through nucleo‐cytoskeletal coupling and affect the nuclear morphology of cancer cells. However, the contribution of actin filament integrity has never been studied directly. It is hypothesized that differences in nuclear deformability of cancer cells are influenced by the integrity of actin filaments. In this study, transparent micropatterned surfaces as simple tools to screen cytoskeletal and nuclear distortions are presented. Surfaces decorated wit...
Contact guidance enhances the quality of a tissue engineered corneal stroma Vrana, E.; Builles, N.; Hindie, M.; Damour, O.; AYDINLI, ATİLLA; Hasırcı, Vasıf Nejat (2008-02-01) Corneal stroma is a very complex structure, composed of 200 lamellae of oriented collagen fibers. This highly complex nature of cornea is known to be important for its transparency and mechanical integrity. Thus, an artificial cornea design has to take into account this complex structure. In this study, behavior of human corneal keratocytes on collagen films patterned with parallel channels was investigated. Keratocytes proliferated well on films and reached confluency after 7 days in the incubation medium....
Nanopatterning of Collagen Scaffolds Improve the Mechanical Properties of Tissue Engineered Vascular Grafts Zorlutuna, P.; Elsheikh, A.; Hasırcı, Vasıf Nejat (2009-04-01) Tissue engineered constructs with cells growing in an organized manner have been shown to have improved mechanical properties. This can be especially important when constructing tissues that need to perform under load, such as cardiac and vascular tissue. Enhancement of mechanical properties of tissue engineered vascular grafts via orientation of smooth muscle cells by the help of topographical cues have not been reported yet. In the present study, collagen scaffolds with 650, 500, and 332.5 nm wide nanocha...
Transient bioelectronics: Electronic properties of silver microparticle-based circuits on polymeric substrates subjected to mechanical load JAMSHIDI, Reihaneh; Çınar, Simge; CHEN, Yuanfen; HASHEMI, Nastaran; MONTAZAMI, Reza (Wiley, 2015-11-15) Transient soft bioelectronics are capable of forming conformal contacts with curvilinear surfaces of biological host tissues and organs. Such systems are often subject to continuous static and dynamic loads from the biological host. In this article, we present investigation of electronic attributes of transient soft bioelectronic circuits subjected to mechanical force and influence of substrate's transiency on the transiency of the whole device; also, characterize and quantify loss of functionality in trigg...

Citation Formats

H. KENAR, C. Y. Ozdogan, C. Dumlu, E. DOĞER, G. T. Kose, and V. N. Hasırcı, “Microfibrous scaffolds from poly(L-lactide-co-epsilon-caprolactone) blended with xeno-free collagen/hyaluronic acid for improvement of vascularization in tissue engineering applications,” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, pp. 31–44, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32107.