Design of a 3D aligned myocardial tissue construct from biodegradable polyesters

Date

2010-03-01

Author

Kenar, H.
KÖSE, GAMZE
Hasırcı, Vasıf Nejat

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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The heart does not regenerate new functional tissue when myocardium dies following coronary artery occlusion, or if it is defective. Ventricular restoration involves excising the infarct and replacing it with a cardiac patch to restore the heart to a more healthy condition. The goal of this study was to design and develop a clinically applicable myocardial patch to replace myocardial infarcts and improve long-term heart function. A basic design composed of 3D microfibrous mats that house mesenchymal stem cells (MSCs) was developed from human umbilical cord matrix (Wharton's Jelly) cells aligned in parallel to each other mimicking the native myocardium. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), poly(L-D,L-lactic acid) (P(L-D,L)LA) and poly(glycerol sebacate) (PGS) were blended and electrospun into aligned fiber mats with fiber diameter ranging between 1.10 and 1.25 mu m. The micron-sized parallel fibers of the polymer blend were effective in cell alignment and cells have penetrated deep within the mat through the fiber interstices, occupying the whole structure; 8-9 cell layers were obtained. Biodegradable macroporous tubings were introduced to serve as nutrient delivery route. It was possible to create a thick myocardial patch with structure similar to the native tissue and with a capability to grow.

Subject Keywords

Marrow-cell transplantation, Hematopoietic stem-cells, Heart-tissue, Extracellular-matrix, Cardiac myocytes, Regeneratİon, Scaffold, Grafts

URI

https://hdl.handle.net/11511/30454

Journal

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

DOI

https://doi.org/10.1007/s10856-009-3917-8

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

H. Kenar, G. KÖSE, and V. N. Hasırcı, “Design of a 3D aligned myocardial tissue construct from biodegradable polyesters,” JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, pp. 989–997, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30454.