Design of a 3D aligned myocardial tissue construct from biodegradable polyesters

2010-03-01
Kenar, H.
KÖSE, GAMZE
Hasırcı, Vasıf Nejat
The heart does not regenerate new functional tissue when myocardium dies following coronary artery occlusion, or if it is defective. Ventricular restoration involves excising the infarct and replacing it with a cardiac patch to restore the heart to a more healthy condition. The goal of this study was to design and develop a clinically applicable myocardial patch to replace myocardial infarcts and improve long-term heart function. A basic design composed of 3D microfibrous mats that house mesenchymal stem cells (MSCs) was developed from human umbilical cord matrix (Wharton's Jelly) cells aligned in parallel to each other mimicking the native myocardium. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), poly(L-D,L-lactic acid) (P(L-D,L)LA) and poly(glycerol sebacate) (PGS) were blended and electrospun into aligned fiber mats with fiber diameter ranging between 1.10 and 1.25 mu m. The micron-sized parallel fibers of the polymer blend were effective in cell alignment and cells have penetrated deep within the mat through the fiber interstices, occupying the whole structure; 8-9 cell layers were obtained. Biodegradable macroporous tubings were introduced to serve as nutrient delivery route. It was possible to create a thick myocardial patch with structure similar to the native tissue and with a capability to grow.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

Suggestions

3d patterned cardiac tissue construct formation using biodegradable materials
Kenar, Halime; Hasırcı, Vasıf Nejat; Department of Biotechnology (2008)
The heart does not regenerate new functional tissue when myocardium dies following coronary artery occlusion, or is defective. Ventricular restoration involves excising the infarct and replacing it with a cardiac patch to restore the heart to a more efficient condition. The goal of this study was to design and develop a myocardial patch to replace myocardial infarctions. A basic design was developed that is composed of 3D microfibrous mats that house mesenchymal stem cells (MSCs) from umbilical cord matrix ...
Design and Development of a Low Cost Device for Bone Fracture Detection Using FFT Technique on MATLAB
Qadir, Zakria; Ali, Muhammad; Nesimoglu, Tayfun (2018-11-02)
Bone fracture is a common problem in daily life which occurs when high pressure is applied on bone or by simple accident and also due to osteoporosis and bone cancer. Different techniques are used today to detect bone fractures such as X-Ray, Computed Tomography (CT-scan), Magnetic Resonance Imaging (MRI) and Ultrasound. Among these four modalities, X-ray diagnosis is commonly used for fracture detection. However, if the fracture is complicated, a CT scan or MRI may be needed for further diagnosis and opera...
Development of vascularized adipose tissue construct based on adipose tissue extracellular matrix and silk fibroin
Kayabölen, Alişan; Tezcaner, Ayşen; Zor, Fatih; Department of Biomedical Engineering (2015)
Adipose tissue engineering is a promising field for regeneration of soft tissue defects. However, vascularization is needed since cells in the middle layer of thick implants cannot reach to nutrient and oxygen by diffusion. Finding a biocompatible scaffold with good mechanical properties is another problem in this field. In this study, we aimed to develop a thick functional vascularized adipose tissue which supports cell viability and functionality with similar mechanical properties with the adipose tissue....
Characterization and identification of human mesenchymal stem cells at molecular level
Aksoy, Ceren; Severcan, Feride; Çetinkaya, Duygu Uçkan; Department of Biotechnology (2012)
Bone marrow mesenchymal stem cells (BM-MSCs) are pluripotent cells that can differentiate into a variety of non-hematopoietic tissues. They also maintain healthy heamatopoiesis by providing supportive cellular microenvironment into BM. In this thesis, MSCs were characterized in terms of their morphological, immunophenotypical and differentiation properties. Then, they were examined by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy together with hierarchical clustering, and FT...
Study on the influence of scaffold inner organization on the treatment of spinal cord injury
Dursun, Tuğba; Hasırcı, Deniz; Yücel, Deniz; Department of Biotechnology (2013)
Permanent loss of neurologic function occurs when the central nervous system (CNS) is injured. Nerve regeneration is extremely limited because of dense scar tissue formation at the lesion site, especially in the spinal cord. For the treatment of this type of injury, different strategies are needed. The emerging approach to solve these problems is to provide a physical support via tubular bridging devices such as “nerve guides”. They are used to bridge the neural gaps in the treatment of spinal cord injury, ...
Citation Formats
H. Kenar, G. KÖSE, and V. N. Hasırcı, “Design of a 3D aligned myocardial tissue construct from biodegradable polyesters,” JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, pp. 989–997, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30454.