Kinetic approach for the purification of nucleotides with magnetic separation

2014-11-01
Tural, Servet
Tural, Bilsen
Ece, Mehmet Sakir
Yetkin, Evren
Özkan, Necati
Show full item record
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
226
views
0
downloads
The isolation of beta-nicotinamide adenine dinucleotide is of great importance since it is widely used in different scientific and technologic fields such as biofuel cells, sensor technology, and hydrogen production. In order to isolate beta-nicotinamide adenine dinucleotide, first 3-aminophenyboronic acid functionalized magnetic nanoparticles were prepared to serve as a magnetic solid support and subsequently they were used for reversible adsorption/desorption of beta-nicotinamide adenine dinucleotide in a batch fashion. The loading capacity of the 3-aminophenyboronic acid functionalized nanoparticles for beta-nicotinamide adenine dinucleotide adsorption was 13.0 mu mol/g. Adsorption kinetic and isotherm studies showed that the adsorption process followed a pseudo-second-order kinetic model and the experimental data can be represented using Langmuir isotherm model. The 3-aminophenyboronic acid functionalized magnetic nanoparticles were proposed as an alternative support for the beta-nicotinamide adenine dinucleotide purification. The results elucidated the significance of magnetic separation as a fast, relatively simple, and low-cost technique. Furthermore, the magnetic supports can be reused at least five times for purification processes.
Reusability, Nucleotides, Magnetic assisted separation, Desorption, Adsorption
https://hdl.handle.net/11511/30593
JOURNAL OF SEPARATION SCIENCE
Graduate School of Natural and Applied Sciences, Article

Suggestions

Biological hydrogen production by using co-cultures of PNS bacteria
Baysal, Görkem; Yücel, Ayşe Meral; Özgür, Ebru; Department of Biotechnology (2012)
Biological hydrogen production is a renewable, carbon-neutral and clean route for hydrogen production. Purple non-sulfur (PNS) bacteria have the ability to produce biohydrogen via photofermentation process. The type of the bacterial strain used in photofermentation is known to have an important effect on hydrogen yield. In this study, the effect of different co-cultures of PNS bacteria on photofermentation process was investigated in search of improving the hydrogen yield. For this purpose, growth, hydrogen...
Experimental investigation on the electrocatalytic behavior of Ag-based oxides, Ag2XO4 (X= Cr, Mo, W), for the oxygen reduction reaction in alkaline media
Hamat, Burcu Arslan; Aydınol, Mehmet Kadri (Elsevier BV, 2020-10-01)
The oxygen reduction (ORR) is one of the most essential electrochemical reactions for the development of promising energy storage and conservation technologies such as metal-air batteries and fuel cells. The slow kinetics of oxygen reactions; however, limits the use of metal-air batteries and fuel cells in demanding applications. The aim of this study is to investigate the electrochemical activity of Ag-based oxides, Ag2XO4 (where X = Cr, Mo, and W), to be used as a catalyst material in these applications. ...
Adsorption properties of boron nitride nanotubes
Khan, Saeed Ahmad; Sezgi, Naime Aslı; Balcı, Fatma Suna; Department of Chemical Engineering (2016)
The developments in nanotechnology in last decades have provided use of nanoparticles for many applications in various areas such as electronics, fuel cells, composites, cosmetics, and biomedical. They have excellent mechanical, thermal, and electrical properties. Nanotechnology is one of the fastest growing areas in materials and engineering science and biotechnology. Nanotubes have been one of the most regarded and studied type of nanoparticles up to now. Boron nitride nanotubes (BNNTs) are an important m...
First principles investigation of hydrogen storage in intermetallic systems
Kınacı, Alper; Aydınol, Mehmet Kadri; Department of Metallurgical and Materials Engineering (2007)
The design and production of efficient metal-hydrides for hydrogen storage is a long standing subject. Over the years, many different types of intermetallic hydride systems were studied and some of them came out to be operable. However, none of them meet all the storage criteria perfectly. In this study, total energies, hydrogen storage capacity and stability of AB (A = Al, Be, Cu, Fe, Ni, Sb, V and B = Ti) type intermetallics were investigated with the goal of spotting a potential hydrogen storage material...
Covalent and non-covalent functionalization of graphene for application in catalysis and device technology: a first principles computational study
Akay, Tuğçe İrfan; Toffoli, Hande; Akata Kurç, Burcu; Department of Micro and Nanotechnology (2017)
In the past few decades, nanomaterials have received increasing attention in various applications such as drug delivery, sensors, hydrogen storage and solar cells. This thesis focuses on the theoretical exploration of pristine and defective graphene in order to highlight the functionalization potential with the use of a wide variety of molecules such as benzene (C6H6), toluene (C7H8), fluorobenzene (C6H5F), benzonitrile (C7H5N) , benzoic acid (C7H6O2), and boron (B) atom. The structures as well as electroni...
Citation Formats
S. Tural, B. Tural, M. S. Ece, E. Yetkin, and N. Özkan, “Kinetic approach for the purification of nucleotides with magnetic separation,” JOURNAL OF SEPARATION SCIENCE, pp. 3370–3376, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30593.
METU IIS - Integrated Information Service open@metu.edu.tr