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Kinetic approach for the purification of nucleotides with magnetic separation
Date
2014-11-01
Author
Tural, Servet
Tural, Bilsen
Ece, Mehmet Sakir
Yetkin, Evren
Özkan, Necati
Metadata
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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The isolation of beta-nicotinamide adenine dinucleotide is of great importance since it is widely used in different scientific and technologic fields such as biofuel cells, sensor technology, and hydrogen production. In order to isolate beta-nicotinamide adenine dinucleotide, first 3-aminophenyboronic acid functionalized magnetic nanoparticles were prepared to serve as a magnetic solid support and subsequently they were used for reversible adsorption/desorption of beta-nicotinamide adenine dinucleotide in a batch fashion. The loading capacity of the 3-aminophenyboronic acid functionalized nanoparticles for beta-nicotinamide adenine dinucleotide adsorption was 13.0 mu mol/g. Adsorption kinetic and isotherm studies showed that the adsorption process followed a pseudo-second-order kinetic model and the experimental data can be represented using Langmuir isotherm model. The 3-aminophenyboronic acid functionalized magnetic nanoparticles were proposed as an alternative support for the beta-nicotinamide adenine dinucleotide purification. The results elucidated the significance of magnetic separation as a fast, relatively simple, and low-cost technique. Furthermore, the magnetic supports can be reused at least five times for purification processes.
Subject Keywords
Reusability
,
Nucleotides
,
Magnetic assisted separation
,
Desorption
,
Adsorption
URI
https://hdl.handle.net/11511/30593
Journal
JOURNAL OF SEPARATION SCIENCE
DOI
https://doi.org/10.1002/jssc.201400648
Collections
Graduate School of Natural and Applied Sciences, Article
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S. Tural, B. Tural, M. S. Ece, E. Yetkin, and N. Özkan, “Kinetic approach for the purification of nucleotides with magnetic separation,”
JOURNAL OF SEPARATION SCIENCE
, pp. 3370–3376, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30593.