Anti-cancer effect of clofazimine as a single agent and in combination with cisplatin on U266 multiple myeloma cell line

Date

2017-04-01

Author

Durusu, Ipek Z.
Husnugil, Hazal H.
ATAŞ, Heval
BİBER, Aysenur
GEREKCİ, Selin
Gulec, Ezgi A.
Özen, Can

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Multiple Myeloma (MM) is a malignant neoplasm of bone marrow plasma B cells with high morbidity. Clofazimine (CLF) is an FDA-approved leprostatic, anti-tuberculosis, and anti-inflammatory drug that was previously shown to have growth suppression effect on various cancer types such as hepatocellular, lung, cervix, esophageal, colon, and breast cancer as well as melanoma, neuroblastoma, and leukemia. The objective of this study was to evaluate the anticancer effect and mechanism of CLF on U266 MM cell line. CLF (10 mu M, 24 h) treatment resulted up to 72% growth suppression on a panel of hematological cell lines. Dose-response study conducted on U266 MM cell line revealed an IC50 value of 9.8 +/- 0.7 mu M. CLF also showed a synergistic inhibition effect in combination with cisplatin. In mechanistic assays, CLF treatment caused mitochondrial membrane depolarization, change in cell membrane asymmetry and increase in caspase-3 activity; indicating to an intrinsic apoptosis mechanism. This study provides new evidence for the anticancer effect of CLF on U266 cell line. Further in vivo and clinical studies are warranted to evaluate its therapeutic potential for MM treatment.

Subject Keywords

Synergism, Apoptosis, Clofazimine, Multiple myeloma, Cancer

URI

https://hdl.handle.net/11511/30855

Journal

LEUKEMIA RESEARCH

DOI

https://doi.org/10.1016/j.leukres.2017.01.019

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

I. Z. Durusu et al., “Anti-cancer effect of clofazimine as a single agent and in combination with cisplatin on U266 multiple myeloma cell line,” LEUKEMIA RESEARCH, pp. 33–40, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30855.