Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Anticancer activity of chlorpromazine on U266 multiple myeloma cell line
Date
2017
Author
Güleç, Aliye Ezgi
Metadata
Show full item record
Item Usage Stats
309
views
0
downloads
Cite This
Multiple Myeloma (MM) is the second most common hematological malignancy caused by malignant growth of plasma B cells. It accounts for 10% of deaths from blood cancers. Although introduction of new drugs has significantly increased the success of chemotherapy, MM remains as an incurable disease with a high relapse rate. Drug repositioning, finding new uses for approved drugs, is a frequently used strategy for the discovery of new chemotherapeutic agents. Since already approved drugs are used, the cost and time spent are considerably reduced. Chlorpromazine (CPZ), which has been used in several drug repositioning studies against different cancer types, is an FDA-approved antipsychotic drug mainly used in the treatment of schizophrenia. In this work, we aim to investigate the anticancer effect and mechanism of CPZ on U266 MM cell line. Firstly, CPZ`s in vitro inhibitory effect at various doses and time points was studied. Then three different apoptosis studies and cell cycle analysis were conducted. Combination potential of CPZ with cisplatin was also investigated during this study. CPZ showed both dose- and time- dependent growth inhibitory effect on U266 MM cell line with an IC50 value of 22.4 ± 0.9 μM. Apoptotic effect of CPZ was indicated by activation of Caspase-3 and change in the cell membrane asymmetry. In addition, CPZ treatment induced cell cycle arrest at G2/M phase. Its combination with cisplatin was moderately synergistic. Based on our preliminary findings, therapeutic potential of CPZ for MM treatment can be investigated with in-depth mechanistic studies and in vivo experiments..
Subject Keywords
Cell lines.
,
Apoptosis.
,
Chlorpromazine.
,
Drug development.
,
Drugs
,
Cancer
URI
http://etd.lib.metu.edu.tr/upload/12621303/index.pdff
https://hdl.handle.net/11511/26646
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Anti-cancer effect of clofazimine as a single agent and in combination with cisplatin on U266 multiple myeloma cell line
Durusu, Ipek Z.; Husnugil, Hazal H.; ATAŞ, Heval; BİBER, Aysenur; GEREKCİ, Selin; Gulec, Ezgi A.; Özen, Can (2017-04-01)
Multiple Myeloma (MM) is a malignant neoplasm of bone marrow plasma B cells with high morbidity. Clofazimine (CLF) is an FDA-approved leprostatic, anti-tuberculosis, and anti-inflammatory drug that was previously shown to have growth suppression effect on various cancer types such as hepatocellular, lung, cervix, esophageal, colon, and breast cancer as well as melanoma, neuroblastoma, and leukemia. The objective of this study was to evaluate the anticancer effect and mechanism of CLF on U266 MM cell line. C...
An update on molecular biology and drug resistance mechanisms of multiple myeloma
Mutlu, Pelin; Gündüz, Ufuk (Elsevier BV, 2015-12)
Multiple myeloma (MM), a neoplasm of plasma cells, is the second most common hematological malignancy. Incidance rates increase after age 40. MM is most commonly seen in men and African-American population. There are several factors to this, such as obesity, environmental factors, family history, genetic factors and monoclonal gammopathies of undetermined significance (MGUS) that have been implicated as potentially etiologic. Development of MM involves a series of complex molecular events, including chromos...
Anti-Cancer Effects of Clofazimine As a Single Agent and in Combination with Cisplatin in Multiple Myeloma
Durusu, Ipek; Hüsnügil, Hepşen Hazal; Atas, Heval; Biber, Aysenur; Gerekci, Selin; Gulec, Aliye Ezgi; Özen, Can (2016-12-02)
Multiple Myeloma (MM) is a malignant neoplasm of bone marrow plasma B cells with high morbidity. Clofazimine (CLF) is an FDA-approved leprostatic, anti-tuberculosis, and anti-inflammatory drug that was previously shown to have growth suppression effect on various cancer types such as hepatocellular, lung, cervix, esophageal, colon, and breast cancer as well as melanoma, neuroblastoma, and leukemia. The objective of this study was to evaluate the anticancer effect and mechanism of CLF on U266 MM cell line. C...
Anticancer effect and combination potential of clofazimine in multiple myeloma
Durusu, İpek Zeynep; Özen, Can; Gündüz, Ufuk; Department of Biotechnology (2016)
Multiple Myeloma (MM) is a malignant neoplasm of bone marrow plasma B cells with high low survival rates. Clofazimine (CLF) is a FDA-approved leprostatic, anti-tuberculous and anti-inflammatory drug. CLF has shown to have growth suppression in various cancers such as hepatocellular, lung, cervix, melanoma, esophageal, colon, leukemia, neuroblastoma and breast. Objective of this study was to investigate anticancer effect and mechanism of Clofazimine on U266 Multiple Myeloma cell line. The relative cell viabi...
Induction of apoptosis and cell cycle arrest on U266 multiple myeloma cell line by prochlorperazine
Hüsnügil, Hepşen Hazal; Özen, Can; Banerjee, Sreeparna; Department of Biochemistry (2016)
Multiple myeloma (MM) is a plasma cell neoplasm accounting for 1% of all malignancies and 13% of hematological malignancies. Despite the introduction of potent anticancer agents, MM remains as an incurable disease. High frequency of relapses and acquisition of resistance to current chemotherapy create a need for the development of novel agents for MM treatment. Prochlorperazine (PCP) is an FDA-approved phenothiazine drug, mainly used for the treatment of chemotherapy-associated nausea and vomiting. In addit...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
A. E. Güleç, “Anticancer activity of chlorpromazine on U266 multiple myeloma cell line,” M.S. - Master of Science, Middle East Technical University, 2017.