Effect of double growth factor release on cartilage tissue engineering

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Date

2013-02-01

Author

Ertan, Ayse Burcu
Yilgor, Pinar
Bayyurt, Banu
Calikoglu, Ayse Ceren
Kaspar, Cigdem
Kök, Fatma Neşe
KÖSE, GAMZE
Hasırcı, Vasıf Nejat

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The effects of double release of insulin-like growth factor I (IGF-I) and growth factor 1 (TGF1) from nanoparticles on the growth of bone marrow mesenchymal stem cells and their differentiation into cartilage cells were studied on PLGA scaffolds. The release was achieved by using nanoparticles of poly(lactic acid-co-glycolic acid) (PLGA) and poly(N-isopropylacrylamide) (PNIPAM) carrying IGF-I and TGF1, respectively. On tissue culture polystyrene (TCPS), TGF-1 released from PNIPAM nanoparticles was found to have a significant effect on proliferation, while IGF-I encouraged differentiation, as shown by collagen type II deposition. The study was then conducted on macroporous (pore size 200400 mu m) PLGA scaffolds. It was observed that the combination of IGF-I and TGF-1 yielded better results in terms of collagen type II and aggrecan expression than GF-free and single GF-containing applications. It thus appears that gradual release of a combination of growth factors from nanoparticles could make a significant contribution to the quality of the engineered cartilage tissue. Copyright (c) 2011 John Wiley & Sons, Ltd.

Subject Keywords

Cartilage tissue engineering, Growth factors, Peptide and protein delivery, Mesenchymal stem cells, Cell differentiation

URI

https://hdl.handle.net/11511/31228

Journal

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE

DOI

https://doi.org/10.1002/term.509

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

A. B. Ertan et al., “Effect of double growth factor release on cartilage tissue engineering,” JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, pp. 149–160, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31228.