Influence of co-culture on osteogenesis and angiogenesis of bone marrow mesenchymal stem cells and aortic endothelial cells

Date

2016-11-01

Author

Pekozer, Gorke Gurel
KÖSE, GAMZE
Hasırcı, Vasıf Nejat

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Co-culture of bone forming cells and endothelial cells to induce pre-vascularization is one of the strategies used to solve the insufficient vascularization problem in bone tissue engineering attempts. In the study, primary cells isolated from 2 different tissues of the same animal, rat bone marrow stem cells (RBMSCs) and rat aortic endothelial cells (RAECs) were co-cultured to study the effects of co-culturing on both osteogenesis and angiogenesis. The formation of tube like structure in 2D culture was observed for the first time in the literature by the co-culture of primary cells from the same animal and also osteogenesis and angiogenesis were investigated at the same time by using this co-culture system. Co-cultured cells mineralized and formed microvasculature beginning from 14 days of incubation. After 28 days of incubation in the osteogenic medium, expression of osteogenic genes in co-cultures was significantly upregulated compared to RBMSCs cultured alone. These results suggest that the co-culture of endothelial cells with mesenchymal stem cells induces both osteogenesis and angiogenesis.

Subject Keywords

Bone tissue engineering, Co-culture, Endothelial cells, Mesenchymal stem cells, Vascularization

URI

https://hdl.handle.net/11511/31069

Journal

MICROVASCULAR RESEARCH

DOI

https://doi.org/10.1016/j.mvr.2016.06.005

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

G. G. Pekozer, G. KÖSE, and V. N. Hasırcı, “Influence of co-culture on osteogenesis and angiogenesis of bone marrow mesenchymal stem cells and aortic endothelial cells,” MICROVASCULAR RESEARCH, pp. 1–9, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31069.