Development of poly (I:C) modified doxorubicin loaded magnetic dendrimer nanoparticles for targeted combination therapy

Date

2014-10-01

Author

Khodadust, Rouhollah
Unsoy, Gozde
Gündüz, Ufuk

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

208
views

0
downloads

The objective of this study was to develop and evaluate the anticancer activity and the safety of a combinational drug delivery system using polyamidoamine (PAMAM) dendrimer-coated iron oxide nanoparticles for doxorubicin and poly I:C delivery in vitro. Dendrimer-coated magnetic nanoparticles (DcMNPs) are suitable for drug delivery system as nanocarriers with their following properties, such as surface functional groups, symmetry perfection, internal cavities, nano-size and magnetization. These nanoparticles could be targeted to the tumor site under a magnetic field since they have a magnetic core. DcMNPs were found as a convenient vehicle for targeted doxorubicin delivery in cancer therapy. Poly (I:C) binding on doxorubicin loaded DcMNPs (DcMNPs-Dox) was reported for the first time in the literature. It was also demonstrated that loading of doxorubicin into the cavities of DcMNPs increases the binding efficiency of poly (I:C) to the surface functional groups of dendrimer up to 10 times. When we compare the in vitro cytotoxic properties of doxorubicin, poly (I:C) and poly (I:C) bound doxorubicin loaded DcMNPs (PIC-DcMNPs-Dox), it was observed that PIC-DcMNPs-Dox show the highest cytotoxic effect by passing the cell resistance mechanisms on doxorubicin resistant MCF7 (MCF7/Dox) cells. Results demonstrated that applying PIC-DcMNPs-Dox would improve the efficacy by increasing the biocompatibility of system in blood stream and the toxicity inside tumor cells. These results provide invaluable information and new insight for the design and optimization of a novel combinational drug delivery system for targeted cancer therapy. (C) 2014 Elsevier Masson SAS. All rights reserved.

Subject Keywords

Cancer, Drug targeting, Dendrimers, Nanoparticles, Efflux pumps, Toxicity

URI

https://hdl.handle.net/11511/31247

Journal

BIOMEDICINE & PHARMACOTHERAPY

DOI

https://doi.org/10.1016/j.biopha.2014.10.009

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

The Synthesis and characterization of doxorubicin and bortezomib loaded magnetic nanoparticles for targeting tumor cells Ünsoy, Gözde; Gündüz, Ufuk; Budak, Güven Gürer; Department of Biotechnology (2013) Chitosan superparamagnetic nanoparticles, loaded with Doxorubicin and Bortezomib were synthesized for treatment of breast and cervical cancers by targeted drug delivery. In vitro cytotoxicity analyses revealed that the efficacy of drugs was highly increased when applied as loaded on nanoparticles. Chitosan superparamagnetic iron oxide nanoparticles (CSMNPs) were in-situ synthesized at different sizes by ionic crosslinking method. The characterization of nanoparticles was performed by XRD, XPS/ESCA, FTIR, TE...
Investigation of the Therapeutic Effects of Palbociclib Conjugated Magnetic Nanoparticles on Different Types of Breast Cancer Cell Lines Parsian, Maryam; MUTLU, PELİN; Taghavi Pourianazar, Negar; Yalcin Azarkan, Serap; Gündüz, Ufuk (2023-01-01) Introduction: Drug targeting and controlled drug release systems in cancer treatment have many advantages over conventional chemotherapy in terms of limiting systemic toxicity, side effects, and overcoming drug resistance. Methods and Results: In this paper, fabricating nanoscale delivery system composed of magnetic nanoparticles (MNPs) covered with poly-amidoamine (PAMAM) dendrimers and using its advantages were fully used to help the chemotherapeutic drug, Palbociclib, effectively reach tumors, specifical...
Synthesis and biological evaluation of novel isoxazole-piperazine hybrids as potential anti-cancer agents with inhibitory effect on liver cancer stem cells Ibis, Kubra; Sinoplu, Esra; ÇALIŞKAN, BURCU; Kahraman, Deniz Cansen; Cetin-Atalay, Rengul; BANOĞLU, ERDEN (2021-10-01) In our effort for the development of novel anticancer therapeutics, a series of isoxazole-piperazine analogues were prepared, and primarily screened for their antiproliferative potential against hepatocellular carcinoma (HCC; Huh7/Mahlavu) and breast (MCF-7) cancer cells. All compounds demonstrated potent to moderate cytotoxicity on all cell lines with IC50 values in the range of 0.09-11.7 mu M. Further biological studies with 6a and 13d in HCC cells have shown that both compounds induced G1 or G2/M arrests...
The Antiproliferative Effect of Celecoxib Loaded pNIPAM Nanoparticles Bayyurt, Banu; Hasırcı, Vasıf Nejat (2012-09-01) The aim of this study was to design a drug delivery system based on poly(N-isopropylacrylamide) (pNIPAM) nanoparticles (NPs). The model drug, Celecoxib, is a cyclooxygenase-2 inhibitor and has a great potential in chemoprevention and treatment of various cancer types, however, the clinical use is limited due to the side effects on the cardiovascular system which is most probably due to the high doses used in clinical applications. In this study, a novel nanoparticle preparation approach, nanoprecipitation, ...
Tailoring the magnetic behavior of polymeric particles for bioapplications YAKAR, ARZU; Tansik, Gulistan; Keskin, Tugba; Gündüz, Ufuk (2013-05-01) In this study, magnetic polymeric nanoparticles were prepared use in for targeted drug delivery. First, iron oxide (Fe3O4) magnetic nanoparticles (MNPs) were synthesized by coprecipitation with ferrous and ferric chloride salts. Then, to render the MNPs hydrophobic, the surfaces were covered with oleic acid. Finally, the hydrophobic MNPs (H-MNPs) were encapsulated with polymer. The emulsion evaporation technique was used for the preparation of polymer-coated H-MNP. Poly(DL-lactide-co-glycolide) (PLGA) and c...

Citation Formats

R. Khodadust, G. Unsoy, and U. Gündüz, “Development of poly (I:C) modified doxorubicin loaded magnetic dendrimer nanoparticles for targeted combination therapy,” BIOMEDICINE & PHARMACOTHERAPY, pp. 979–987, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31247.