The Antiproliferative Effect of Celecoxib Loaded pNIPAM Nanoparticles

The aim of this study was to design a drug delivery system based on poly(N-isopropylacrylamide) (pNIPAM) nanoparticles (NPs). The model drug, Celecoxib, is a cyclooxygenase-2 inhibitor and has a great potential in chemoprevention and treatment of various cancer types, however, the clinical use is limited due to the side effects on the cardiovascular system which is most probably due to the high doses used in clinical applications. In this study, a novel nanoparticle preparation approach, nanoprecipitation, was used. The amount of crosslinker affected the size, encapsulation efficiency, loading and release rate of NPs. The antiproliferative effect of Celecoxib was tested on human osteosarcoma cells, Saos-2, and the nanoparticles were found to have cytotoxicity. Celecoxib loaded pNIPAM NPs had higher cytotoxicity and the cells treated with this formulation showed abnormal nuclear and cytoskeletal morphology indicating apoptosis. The nanoparticles were detected within the cytoplasm and their distribution differed depending on whether NP is loaded with Celecoxib or not. The drug delivery system developed in this study appears to have a potential as an antiproliferative tool in various applications such as prevention of restenosis or biofilm formation on biomaterials.


Study on physiochemical structure and in vitro release behaviors of doxycycline-loaded PCL microspheres
AYDIN, Ozlem; AYDIN, Baran; Tezcaner, Ayşen; Keskin, Dilek (2015-04-10)
This study aimed to develop drug delivery system of doxycycline-loaded polycaprolactone (PCL) microspheres. The investigated microsphere formulation can be considered for local application in bone infections and degenerative joint diseases, which generally require long-term treatments via systemic drugs. PCL-14 kDa and 65 kDa were used in microsphere preparation. Before release, the microspheres were characterized by scanning electron microscopy, differential scanning calorimetry, and X-ray photoelectron sp...
Biopolymer based micro/nanoparticles as drug carriers for the treatment of skin diseases
Eke, Gözde; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin; Department of Micro and Nanotechnology (2011)
Controlled drug delivery systems are becoming increasingly interesting with the contribution of nanotechnology. In the case of transdermal applications the greatest limitation is the highly impermeable outermost layer of the skin, the stratum corneum. One promising method of controlled transdermal drug delivery of the skin therapeutics is the use of nanoparticles as carriers. Encapsulation of the drug, as opposed to classical topical application of creams or emulsions, allows the drug to diffuse into hair f...
Development of poly (I:C) modified doxorubicin loaded magnetic dendrimer nanoparticles for targeted combination therapy
Khodadust, Rouhollah; Unsoy, Gozde; Gündüz, Ufuk (2014-10-01)
The objective of this study was to develop and evaluate the anticancer activity and the safety of a combinational drug delivery system using polyamidoamine (PAMAM) dendrimer-coated iron oxide nanoparticles for doxorubicin and poly I:C delivery in vitro. Dendrimer-coated magnetic nanoparticles (DcMNPs) are suitable for drug delivery system as nanocarriers with their following properties, such as surface functional groups, symmetry perfection, internal cavities, nano-size and magnetization. These nanoparticle...
The effects of palbociclib on 2d and 3d cell cultures and doxorubicin resistance
Yalçın, Gizem Damla; Gündüz, Ufuk; Department of Molecular Biology and Genetics (2020)
Cell culture facilitates studying the mechanism of diseases and finding new treatment strategies. Traditionally used two­dimensional cell culture model is not quite sufficient in some studies due to the factors such as lack of cell to cell and cell­extracellular matrix interactions and changing cell morphology. Because of the limitations in 2D cell culture, nowadays three­dimensional cell cultures gain importance. The present study aims to identify the effect of Palbociclib on different cells when they cult...
Tailoring the magnetic behavior of polymeric particles for bioapplications
YAKAR, ARZU; Tansik, Gulistan; Keskin, Tugba; Gündüz, Ufuk (2013-05-01)
In this study, magnetic polymeric nanoparticles were prepared use in for targeted drug delivery. First, iron oxide (Fe3O4) magnetic nanoparticles (MNPs) were synthesized by coprecipitation with ferrous and ferric chloride salts. Then, to render the MNPs hydrophobic, the surfaces were covered with oleic acid. Finally, the hydrophobic MNPs (H-MNPs) were encapsulated with polymer. The emulsion evaporation technique was used for the preparation of polymer-coated H-MNP. Poly(DL-lactide-co-glycolide) (PLGA) and c...
Citation Formats
B. Bayyurt and V. N. Hasırcı, “The Antiproliferative Effect of Celecoxib Loaded pNIPAM Nanoparticles,” JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING, pp. 220–227, 2012, Accessed: 00, 2020. [Online]. Available: