A high-throughput microfluidic rare cell enrichment system based on dielectrophoresis and filtering

Date

2016-05-27

Author

Ozkayar, Gurhan
Yalcin, Yagmur Demircan
Ozgur, Ebru
Gündüz, Ufuk
Külah, Haluk

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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In this study, a MEMS-based microfluidic device combining DEP-based cell manipulation with size-based filtration for the enrichment of CTCs from blood with high-throughput was developed. Positive-DEP (pDEP) force and the hydrodynamic force have been used to fine-tune the cell movement over the planar electrodes (sliding) at a high flow rate (30 mu l/min). While smaller sized RBCs passed through the gaps and were directed to the waste channels, cancer cells (K562 cells) were filtered and directed to the cancer cell outlet. A cell enrichment factor and depletion rate of RBCs were calculated as 1.45 and 60%, respectively.

Subject Keywords

Dielectrophoresis (DEP), Rare cell enrichment, Blood, Cell slide

URI

https://hdl.handle.net/11511/31251

DOI

https://doi.org/10.1016/j.protcy.2017.04.076

Collections

Graduate School of Natural and Applied Sciences, Conference / Seminar

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Citation Formats

G. Ozkayar, Y. D. Yalcin, E. Ozgur, U. Gündüz, and H. Külah, “A high-throughput microfluidic rare cell enrichment system based on dielectrophoresis and filtering,” 2016, vol. 27, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31251.