A high-throughput microfluidic rare cell enrichment system based on dielectrophoresis and filtering

Ozkayar, Gurhan
Yalcin, Yagmur Demircan
Ozgur, Ebru
Gündüz, Ufuk
Külah, Haluk
In this study, a MEMS-based microfluidic device combining DEP-based cell manipulation with size-based filtration for the enrichment of CTCs from blood with high-throughput was developed. Positive-DEP (pDEP) force and the hydrodynamic force have been used to fine-tune the cell movement over the planar electrodes (sliding) at a high flow rate (30 mu l/min). While smaller sized RBCs passed through the gaps and were directed to the waste channels, cancer cells (K562 cells) were filtered and directed to the cancer cell outlet. A cell enrichment factor and depletion rate of RBCs were calculated as 1.45 and 60%, respectively.


A smart bilayer scaffold of elastin-like recombinamer and collagen for soft tissue engineering
Kinikoglu, Beste; Carlos Rodriguez-Cabello, Jose; Damour, Odile; Hasırcı, Vasıf Nejat (2011-06-01)
Elastin-like recombinamers (ELRs) are smart, protein-based polymers designed with desired peptide sequences using recombinant DNA technology. The aim of the present study was to produce improved tissue engineering scaffolds from collagen and an elastin-like protein tailored to contain the cell adhesion peptide RGD, and to investigate the structural and mechanical capacities of the resulting scaffolds (foams, fibers and foam-fiber bilayer scaffolds). The results of the scanning electron microscopy, mercury p...
A novel multi-electrochromic polymer based on selenophene and benzotriazole via electrochemical and chemical polymerization
Demir, Fatma; Keles, Duygu; Karabag, Aliekber; Çırpan, Ali; Toppare, Levent Kamil (Informa UK Limited, 2019-03-04)
In this study, a novel donor-acceptor type monomer was designed based on selenophene and benzotriazole with a bulky pendant group and synthesized through Stille coupling reaction. The monomer was polymerized electrochemically by using cyclic voltammetry and also chemically by oxidation in the presence of FeCl3. Both polymers were then compared in terms of their optical properties, electrochemical and spectroelectrochemical behaviors, kinetic and colorimetric properties and surface morphologies. Independent ...
A triazoloquinoxaline and benzodithiophene bearing low band gap copolymer for electrochromic and organic photovoltaic applications
HACIOGLU, Serife O.; UNLU, Naime A.; AKTAS, Ece; HIZALAN, Gonul; Yildiz, Esra D.; Çırpan, Ali; Toppare, Levent Kamil (2017-06-01)
A new triazoloquinoxaline and benzodithiophene based copolymer was synthesized to investigate its electro-chemical, optical and photovoltaic behaviors. According to the polymer design, combination of two acceptor units (benzotriazole and quinoxaline) which contribute imine bonds to the structure and a triazoloquinoxaline unit for enhancing electron accepting ability was pursued. As a result of electrochemical studies, the copolymer PTQBDT has a low lying HOMO energy level as - 5.23 eV which increases the ch...
A novel architecture based on a conducting polymer and calixarene derivative: its synthesis and biosensor construction
GOKOGLAN, Tugba Ceren; SOYLEMEZ, Saniye; KESIK, Melis; UNAY, Hande; Sayin, Serkan; Yildiz, Huseyin Bekir; Çırpan, Ali; Toppare, Levent Kamil (2015-01-01)
In this study, a novel amperometric glucose biosensor based on a selenium comprising conducting polymer and calixarene was developed. Firstly, poly(2-(2-octyldodecyl)-4,7-di(selenoph-2-yl)-2H-benzo[d][1,2,3]-triazole), poly((SBTz)) was electrodeposited onto a graphite electrode by an electropolymerization technique. Then, a newly synthesized calixarene and gold nanoparticle (AuNP) mixture was used for the improvement of biosensor characteristics. GOx, as a model enzyme was immobilized on the modified electr...
A benzimidazole-based turn-off fluorescent sensor for selective detection of mercury (II)
ERGÜN, EMİNE GÜL CANSU; Ertaş, Gülay; Eroglu, Deniz (Elsevier BV, 2020-05-01)
In this study, potential fluorescent sensing ability of a donor-acceptor-donor type conjugated molecule over various metal ions (Ni(II), Zn(II), Cu(II), Cd(II), Pb(II), Fe(II), Fe(III), As(III), Se(IV), Hg(I) and Hg(II)) was investigated. The sensor basically includes benzimidazole and 3,4-propylenedioxythiophene as the acceptor and donor units, respectively. Solution of the sensor in tetrahydrofuran exhibits an emission with having a maximum at 632 nm. Since having insolubility of some ions in tetrahydrofu...
Citation Formats
G. Ozkayar, Y. D. Yalcin, E. Ozgur, U. Gündüz, and H. Külah, “A high-throughput microfluidic rare cell enrichment system based on dielectrophoresis and filtering,” 2016, vol. 27, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31251.