Biocompatibility of Dead Sea Water and retinyl palmitate carrying poly(3-hydroxybutyrate-co-3-hydroxyvalerate) micro/nanoparticles designed for transdermal skin therapy

Date

2015-09-01

Author

Eke, Gozde
Goni-de-Cerio, Felipe
Suarez-Merino, Blanca
Hasırcı, Nesrin
Hasırcı, Vasıf Nejat

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

224
views

0
downloads

In this study, novel drug carriers were developed for the treatment of skin conditions such as psoriasis, aging, or ultraviolet damage using micro/nanocapsules and micro/nanospheres of poly(3-hydroxybutyrate-co-3-hydroxyvalerate). The sizes of the particles were in the micron range and were loaded with retinyl palmitate and Dead Sea Water. In some tests, MgCl2 was used as a substitute for Dead Sea Water for accurate determination of released ions of Dead Sea Water. Encapsulation efficiency and loading of water-soluble excipients Dead Sea Water and MgCl2 were almost eight times lower than the hydrophobic compound retinyl palmitate. The particles were not cytotoxic as determined with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test using L929 mouse fibroblasts, BALB/3T3 mouse embryo fibroblasts, and HaCaT human keratinocytes. Ames test showed that the carriers were not genotoxic. The particles penetrated the membrane of human osteosarcoma cells Saos 2 and accumulated in their cytoplasm. No reactive oxygen species production could be detected which indicated low or no inflammatory response toward the particles. In the tests with intact human skin, 1.2% of the retinyl palmitate-loaded poly(3-hydroxybutyrate-co-3-hydroxyvalerate) particles penetrated into the human skin, but when the skin was without stratum corneum and increased to 6.9%. In conclusion, these carriers have shown a significant potential as topical drug delivery systems in the personalized treatment of skin diseases because their contents could be modified according to a patient's needs and several drugs could be loaded in one type of microparticle, or several populations, each carrying a different drug, can be used in the treatment.

Subject Keywords

Drug delivery systems, Biopolymers, Microparticlest, Nanoparticles, Skin diseases, Personalized treatment

URI

https://hdl.handle.net/11511/31335

Journal

JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS

DOI

https://doi.org/10.1177/0883911515585183

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Bioactive agent carrying PLGA nanoparticles in the treatment of skin diseases Küçükturhan, Aysu; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat; Department of Biomedical Engineering (2012) The aim of this study was to develop drug delivery system based on poly(lactic acid-co-glycolic acid) (PLGA) nanoparticles (NPs) to achieve personalized treatment of selected skin disorders, like photo-aging, psoriasis and atopic dermatitis. Dead Sea Water (DSW) and Retinyl Palmitate (RP) were used as active agents and they were loaded in PLGA NPs prepared either as spheres or capsules by o/w or w/o/w methods. MgCl2 and bovine serum albumin (BSA) served as model active compounds. The diameter of the NPs was...
Synthesis and characterization of polyethylene glycol coated magnetic nanoparticles and their use for anti-cancer drug delivery Erdem, Murat; Gündüz, Ufuk; Azarkan, Serap Yalçın; Department of Biology (2014) Although conventional chemotherapy is the most valid method to cope with cancer, it has many drawbacks such as decrease in production of blood cells, inflammation of the lining of the digestive tract, hair loss etc. Reasons for its side effects are that drugs used in chemotherapy are distributed evenly within the body of a patient and cannot distinguish the cancer cells from the healthy ones. To decrease the negative effects of chemotherapeutic drugs and to increase their efficiency, many drug delivery syst...
Synthesis and characterization of fatty acid based hyperbranched polymers for anti-cancer drug delivery Güç, Esra; Gündüz, Ufuk; Department of Biology (2008) Conventional methods of chemotherapy requires novel therapy systems due to serious side effects and inefficiency of drug administration. In recent years many studies are carried out to improve drug delivery systems. Polymers are one of the most important elements for drug delivery research due to their versatility. By the discovery of dendritic polymers, drug delivery studies gained a new vision. Highly branched monodisperse structure, multiple sites of attachment, well-defined size and controllable physica...
Nanocrystal Encoded FluorescentMicrobeads for Proteomics AntibodyProfiling and Diagnostics ofAutoimmune Diseases Sukhanova, Alyona; Susha , Andrei; Bek, Alpan; Mayilo, Sergiy; Rogach , Andrey; Feldmann, Jochen; Oleinikov, Vladimir; Reveil, Brigitte; Donvito, Beatrice; Cohen, Jacques; Nabiev, İgor (2007-01-01) The first application of nanocrystal (NC)-encoded microbeads to clinical proteomics is demonstrated by multiplexed detection of circulating autoantibodies, markers of systemic sclerosis. Two-color complexes, consisting of NC-encoded, antigen-covered beads, anti-antigen antibody or clinical serum samples, and dye-tagged detecting antibodies, were observed using flow cytometry assays and on the surface of single beads. The results of flow cytometry assays correlated with the ELISA technique and provided clear...
Targeted co-delivery of doxorubicin and TPGS to breast cancer cells by PLGA coated magnetic nanoparticles Metin, Esra; Gündüz, Ufuk; Mutlu, Pelin; Department of Biotechnology (2017) Although conventional chemotherapy is the most common method for cancer treatment, it has several side effects such as neuropathy, alopecia and cardiotoxicity. Since the drugs are given to body systemically, normal cells also effect as cancer cells. However, in recent years, targeted drug delivery has been developed to overcome these drawbacks.The targeting strategy can be changed depending on carrier types, but magnetic nanoparticles are commonly preferred due to their easy targetable features by using ext...

Citation Formats

G. Eke, F. Goni-de-Cerio, B. Suarez-Merino, N. Hasırcı, and V. N. Hasırcı, “Biocompatibility of Dead Sea Water and retinyl palmitate carrying poly(3-hydroxybutyrate-co-3-hydroxyvalerate) micro/nanoparticles designed for transdermal skin therapy,” JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, pp. 455–471, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31335.