Effect of cobalt-60 (gamma radiation) on multidrug-resistant multiple myeloma cell lines

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Date

2011-07-01

Author

Mutlu, Pelin
Ural, A. Ugur
Avcu, Ferit
Dirican, Bahar
Beyzadeoglu, Murat
Gündüz, Ufuk

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Emergence of resistance to chemotherapy and radiotherapy is a major obstacle for the successful treatment of MM (multiple myeloma). Prednisone, vincristine and melphalan are commonly used chemotherapeutic agents for the treatment of MM. In the current study, we examined the presence of possible cross-resistance between these drugs and gamma (gamma) radiation. Prednisone, vincristine and melphalan resistant RPMI-8226 and U-266 MM cells were generated by stepwise increasing concentrations of the drugs. The sensitive and resistant cells were exposed to 200- and 800 cGy gamma radiation, and proliferation was examined by XTT {2,3-bis(2-methoxy-4-nitro-5-sulfopheny1)-5-Rphenylamino)carbonyl]-2H-tetrazolium hydroxide) assay. The results showed that Prednisone- and melphalan-resistant RPMI-8226 cells were also cross-resistant to 200 and 800 cGy gamma radiation application, while vincristine-resistant cells did not show resistance. On the other hand, Prednisone-, vincristine- and melphalan-resistant U-266 cells showed cross-resistance to 200- and 800 cGy gamma radiation application. These results demonstrated that MM cells resistant to anticancer agents respond to radiation in different levels. These findings may be important in the clinical applications of radiation therapy in the treatment of vincristine resistant MM.

Subject Keywords

Multiple myeloma, Radioresistance, Drug resistance, Drug resistance

URI

https://hdl.handle.net/11511/31511

Journal

CELL BIOLOGY INTERNATIONAL

DOI

https://doi.org/10.1042/cbi20100061

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

P. Mutlu, A. U. Ural, F. Avcu, B. Dirican, M. Beyzadeoglu, and U. Gündüz, “Effect of cobalt-60 (gamma radiation) on multidrug-resistant multiple myeloma cell lines,” CELL BIOLOGY INTERNATIONAL, pp. 721–725, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31511.