Investigation of the effects of initial substrate and biomass concentrations and light intensity on photofermentative hydrogen gas production by Response Surface Methodology

2015-04-27
Akman, Melih Can
Bayramoğlu, Tuba Hande
Gündüz, Ufuk
EROĞLU, İNCİ
Show full item record
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
255
views
0
downloads
Biohydrogen, which can be produced by dark fermentation and photofermentation processes, is a renewable and clean approach for hydrogen production. In this study, it was aimed to determine the operational conditions which satisfy the highest photofermentative hydrogen production rate in batch reactors. To that purpose, the effects of initial substrate concentration, initial volatile suspended solids (VSS) concentration and light intensity on photofermentation process, and their interactive effects were investigated by using Response Surface Methodology (RSM). The photofermentative process was followed by using pure strain of purple non-sulfur (PNS) bacteria: Rhodobacter capsulatus DSM 1710. RSM results revealed that the highest hydrogen production rate of 1.04 mmol/L-reactor.h can be obtained when acetate concentration, initial R. capsulatus concentration and the light intensity values were 35.35 mM, 0.27 g VSS/L and 263.6 W/m(2) (3955 lux), respectively. Optimum Substrate/Initial biomass concentration ratio (S/X-o) was found as 7.7 g acetate/g VSS (8.3 g Chemical Oxygen Demand/g VSS). Copyright (C) 2015, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
Biohydrogen, COD-chemical oxygen demand, Photofermentation, Rhodobacter capsulatus, Response surface methodology
https://hdl.handle.net/11511/31573
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
Graduate School of Natural and Applied Sciences, Article

Suggestions

Investigation of the effects of initial substrate and biomass concentrations and light intensity on photofermentative hydrogen gas production
Akman, Melih Can; Erguder, Tuba Hande; Gündüz, Ufuk; Eroglu, Inci (2014-01-01)
Biohydrogen, which can be produced by dark fermentation and photofermentation processes, is a renewable and clean approach for hydrogen production. In this study, it was aimed to determine the operational conditions which satisfy the highest photofermentative hydrogen production rate in batch reactors. To that purpose, the effects of initial substrate concentration, initial volatile suspended solids (VSS) concentration and light intensity on photofermentation process were investigated by using Response Surf...
Evaluation of hydrogen production by Rhodobacter sphaeroides OU001 and its hupSL deficient mutant using acetate and malate as carbon sources
KARS, GÖKHAN; Gündüz, Ufuk; Yucel, Meral; Rakhely, Gabor; Kovacs, Kornel L.; Eroglu, Inci (2009-03-01)
Rhodobacter sphaeroides O.U.001 is one of the candidates for photobiological hydrogen production among purple non-sulfur bacteria. Hydrogen is produced by Mo-nitrogenase from organic acids such as malate or lactate. A hupSL in frame deletion mutant strain was constructed without using any antibiotic resistance gene. The hydrogen production potential of the R. sphaeroides O.U.001 and its newly constructed hupSL deleted mutant strain in acetate media was evaluated and compared with malate containing media. Th...
Significance of carbon to nitrogen ratio on the long-term stability of continuous photofermentative hydrogen production
Androga, Dominic Deo; Ozgur, Ebru; Eroglu, Inci; Gündüz, Ufuk; Yucel, Meral (2011-12-01)
The stable and optimized operation of photobioreactors (PBRs) is the most challenging task in photofermentative biological hydrogen production. The carbon to nitrogen ratio (C/N) in the feed is a critical parameter that significantly influences microbial growth and hydrogen production. In this study, the effects of changing the C/N ratio to achieve stable biomass and continuous hydrogen production using fed-batch cultures of Rhodobacter capsulatus YO3 (uptake hydrogenase deleted, hup-) were investigated. Th...
Study of adsorption characteristics of long chain alkyl amine and petroleum sulfonate on silicates by electrokinetic potential, microflotation, FTIR, and AFM analyses
ÖZÜN, Savaş; Atalay, M. Umit; Demirci, Şahinde (Informa UK Limited, 2019-05-19)
The long-chain alkyl amines and petroleum sulfonates are mostly used to remove unwanted minerals from feldspar ores in acidic pHs. In this study, their adsorption characteristics on pure albite and quartz were investigated by electrokinetic potential measurements, microflotation tests, Fourier transform infrared spectroscopy, and atomic force microscopy studies. According to the results, amine had strong influence on zeta potentials of both albite and quartz turning them positive and resulting over 90% flot...
Implementation and analysis of temperature control strategies for outdoor photobiological hydrogen production
ANDROGA, DOMINIC DEO; UYAR, BAŞAR; Koku, Harun; EROĞLU, İNCİ (2016-12-01)
For outdoor photobiological hydrogen production, the effective control of temperature in photobioreactors is a challenge. In this work, an internal cooling system for outdoor tubular photobioreactors was designed, built, and tested. The temperatures in the reactors with bacteria were consistently higher than those without bacteria, and were also strongly influenced by solar irradiation and ambient air temperature. The cooling protocol applied successfully kept the reactor temperatures below the threshold li...
Citation Formats
M. C. Akman, T. H. Bayramoğlu, U. Gündüz, and İ. EROĞLU, “Investigation of the effects of initial substrate and biomass concentrations and light intensity on photofermentative hydrogen gas production by Response Surface Methodology,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 5042–5049, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31573.
METU IIS - Integrated Information Service open@metu.edu.tr