Significance of carbon to nitrogen ratio on the long-term stability of continuous photofermentative hydrogen production

Date

2011-12-01

Author

Androga, Dominic Deo
Ozgur, Ebru
Eroglu, Inci
Gündüz, Ufuk
Yucel, Meral

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The stable and optimized operation of photobioreactors (PBRs) is the most challenging task in photofermentative biological hydrogen production. The carbon to nitrogen ratio (C/N) in the feed is a critical parameter that significantly influences microbial growth and hydrogen production. In this study, the effects of changing the C/N ratio to achieve stable biomass and continuous hydrogen production using fed-batch cultures of Rhodobacter capsulatus YO3 (uptake hydrogenase deleted, hup-) were investigated. The experiments were carried out in 8 L panel PBRs operated in indoor conditions under continuous illumination and controlled temperature. Culture media containing different acetate (40-80 mM) and glutamate (2-4 mM) concentrations were used to study the effects of changing the C/N ratio on biomass growth and hydrogen production. Stable biomass concentration of 0.40 g dry cell weight per liter culture (gDCW/L(c)) and maximum hydrogen productivity of 0.66 mmol hydrogen per liter culture per hour (mmol/L(c)/h) were achieved during fed-batch operation with media containing 40 mM acetate and 4 mM glutamate, C/N = 25, for a period of over 20 days. A study on the effect of biomass recycling on biomass growth and hydrogen production showed that the feedback of cells into the photobioreactor improved biomass stability during the fed-batch operation but decreased hydrogen productivity. Copyright (C) 2011, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

Subject Keywords

Panel photobioreactor, Photofermentation, C/N, Rhodobacter capsulatus, Biohydrogen

URI

https://hdl.handle.net/11511/32111

Journal

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY

DOI

https://doi.org/10.1016/j.ijhydene.2011.09.043

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

D. D. Androga, E. Ozgur, I. Eroglu, U. Gündüz, and M. Yucel, “Significance of carbon to nitrogen ratio on the long-term stability of continuous photofermentative hydrogen production,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 15583–15594, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32111.