Drug resistant MCF-7 cells exhibit epithelial-mesenchymal transition gene expression pattern

Iseri, Ozlem Darcansoy
Kars, Meltem Demirel
Arpaci, Fikret
Atalay, Can
Pak, Isin
Gündüz, Ufuk
Purpose: Multidrug resistance is resistance to structurally unrelated anticancer agents. Large-scale expression analysis by using high-density oligonucleotide microarrays may provide information about new candidate genes contributing to MDR. This study demonstrates alterations in expression levels of several genes related to epithelial-mesenchymal transition (EMT) in paclitaxel, docetaxel, and doxorubicin resistant MCF-7 cells.


Protein kinase D2 silencing reduced motility of doxorubicin-resistant MCF7 cells
Alpsoy, Aktan; Gündüz, Ufuk (2015-06-01)
Success of chemotherapy is generally impaired by multidrug resistance, intrinsic resistance, or acquired resistance to functionally and structurally irrelevant drugs. Multidrug resistance emerges via distinct mechanisms: increased drug export, decreased drug internalization, dysfunctional apoptotic machinery, increased DNA damage repair, altered cell cycle regulation, and increased drug detoxification. Several reports demonstrated that multidrug resistance is a multifaceted problem such that multidrug resis...
Drug Resistant MCF-7 Cell Lines Also Developed Cross-Resistance to Structurally Unrelated Anticancer Agents
Iseri, Ozlem D.; Kars, Meltem D.; Eroglu, Seckin; Gündüz, Ufuk (2009-03-01)
The cells developing resistance to an applied drug may also present cross-resistance to other anticancer drugs which are not applied. In this study, the development of cross-resistance in paclitaxel (MCF-7/Pac), docetaxel (MCF-7/Doc), vincristine (MCF-7/Vinc) and doxorubicin (MCF-7/Dox) resistant MCF-7 cells to selective anticancer drugs, tamoxifen and all trans-retinoic acid (ATRA) were investigated. Combined antiproliferative effects of these drugs in different combinations were also evaluated by checkerb...
Reversal of breast cancer resistance protein mediated multidrug resistance in MCF7 breast adenocarcinoma cell line
Urfalı, Çağrı; Gündüz, Ufuk; Department of Biology (2011)
Resistance to various chemotherapeutic agents is a major problem in success of cancer chemotherapy. One of the primary reasons of development of multidrug resistance (MDR) is the overexpression of ATP binding cassette (ABC) transporter proteins. Breast cancer resistance protein (BCRP) belongs to ABC transporter family and encoded by ABCG2 gene. BCRP is mainly expressed in MDR1 (P-glycoprotein) lacking breast cancer cells. Overexpression of BCRP leads to efflux of chemotherapeutic agents at higher rates, the...
Reversal of multidrug resistance in mcf-7 breast adenocarcinoma cell line by silencing interleukin 6 with RNA interference
Çakmak, Neşe; Gündüz, Ufuk; Department of Biology (2013)
Multidrug resistance (MDR) in cancer is characterized by development of resistance to several unrelated drugs upon long time administration of a certain type of chemotherapeutic agent. In doxorubicin resistant MCF-7 cell line, resistance is developed mainly by upregulation of MDR1 gene which encodes an ABC transporter protein known as P-glycoprotein. Interleukin 6 (IL-6) is a cytokine which acts as a growth factor for certain cell types including some cancer cells. IL-6 is found at high levels in cancer pat...
Downregulation of Programmed Cell Death 10 (PDCD 10) Expression Confers Doxorubicin Resistance in Breast Cancer Cells
Mamatoğlu, Çağrı Urfalı; Kazan, Hasan Hüseyin; Gündüz, Ufuk (null; 2017-10-08)
Dysregulated apoptosis is one of the major drug resistance mechanisms. Programmed cell death 10 (PDCD10) is a novel apoptosis regulator, involving in modulation of apoptosis and cell survival. Despite its importance in apoptotic regulation, the role of PDCD10 in drug resistance has not been understood yet. We screened parental and doxorubicin-resistant MCF7, HeLa and K562 cells for PDCD10 expression. Our results showed that PDCD10 expression is cell-specific; significantly downregulated in MCF7 and upregula...
Citation Formats
O. D. Iseri, M. D. Kars, F. Arpaci, C. Atalay, I. Pak, and U. Gündüz, “Drug resistant MCF-7 cells exhibit epithelial-mesenchymal transition gene expression pattern,” BIOMEDICINE & PHARMACOTHERAPY, pp. 40–45, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31655.