Effects of ammonium ion, acetate and aerobic conditions on hydrogen production and expression levels of nitrogenase genes in Rhodobacter sphaeroides OU001

Date

2009-11-01

Author

Akkose, Sevilay
Gündüz, Ufuk
Yucel, Meral
Eroglu, Inci

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

134
views

0
downloads

In the present study, expression levels of nitrogenase encoding nifH and control genes nifA and prrA were examined at different physiological conditions in Rhodobacter sphaeroides O.U.001. In addition to variations in expression levels, changes in hydrogen production and growth were also investigated in response to different concentrations of ammonium source, acetate and aerobic conditions.

Subject Keywords

Rhodobacter sphaeroides OU001, Biohydrogen, Nitrogenase, Nif H, NifA, PrrA

URI

https://hdl.handle.net/11511/32259

Journal

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY

DOI

https://doi.org/10.1016/j.ijhydene.2009.08.040

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Effect of salt stress on antioxidant defense systems of sensitive and resistance cultivars of lentil(Lens culinaris M.) Cicerali, Işın Nur; Yücel, Ayşe Meral; Department of Biotechnology (2004) In this study, two lentil cultivars (Lens culinaris, Medik.) (ILL5582-salt tolerant and ILL590) were characterized and compared due to their NaCl susceptibility and antioxidant mechanism was examined under laboratory conditions. Physiological parameters such as wet-dry weight, root-shoot lengths, cell membrane stability, lipid peroxidation in terms of malondialdehyde (MDA), H2O2, proline contents were determined. The activities of antioxidant enzymes such as superoxide dismutase (SOD: EC 1.15.1.1), catalase...
Effects of ph on human growth hormone production by pichia pastoris considering the expression levels of regulatory genes Bayraktar, Eda; Çalık, Pınar; Department of Chemical Engineering (2009) In this study, the aim was to investigate the effects of pH on therapeutically important protein, recombinant human growth hormone (rhGH), production by Pichia pastoris considering the expression levels of regulatory genes. In this frame, firstly the host microorganism was selected between two different methanol utilization phenotypes of P. pastoris, Mut+ and MutS on media containing glycerol/methanol or sorbitol/methanol. The highest rhGH production, 120 g L-1, and hGH gene expression, 9.84x109 copies mg-1...
Effects of carbon sources and feeding strategies on human growth hormone production by metabolically engineered pichia pastoris Açık, Eda; Çalık, Pınar; Department of Chemical Engineering (2009) In this study, effects of different carbon sources and their feeding strategies on recombinant human growth hormone (rhGH) production by Pichia pastoris were investigated by means of cell growth, recombinant protein production and expression levels of hGH and alcohol oxidase (AOX) genes. In this content, firstly, the strain to be used for high level rhGH production was selected between the two phenotypes, i.e., P. pastoris hGH-Mut+ and P. pastoris hGH-MutS. In this selection both phenotypes were compared in...
Role of the cmcH-ccaR intergenic region and ccaR overexpression in cephamycin C biosynthesis in Streptomyces clavuligerus Kurt, Aslihan; Alvarez-Alvarez, Ruben; Liras, Paloma; Özcengiz, Gülay (Springer Science and Business Media LLC, 2013-07-01) The effect of the CcaR regulatory protein on expression of the cephamycin C gene cluster is studied. Quantitative reverse transcription PCR (qRT-PCR) expression analysis of the cephamycin biosynthesis genes in the ccaR-disrupted strain, S. clavuligerus ccaR::aph, revealed that in the absence of CcaR, the lat and cmcI genes expression was reduced 2,200-and 1,087-fold compared with the wild type. Expression of pcbAB-pcbC-cefD-cefE-cmcJ-cmcH and blp was 225- to 359-fold lower, while expression of pcbR-pbpA-bla...
Hydrogen production and transcriptional analysis of nifD, nifK and hupS genes in Rhodobacter sphaeroides OU001 grown in media with different concentrations of molybdenum and iron Kars, Gokhan; Gündüz, Ufuk; Yucel, Meral; Turker, Lemi; Eroglu, Inci (2006-09-01) Rhodobacter sphaeroides O.U.001 was grown in media with different concentrations of molybdenum and iron to study the expression level of nifD and nifK genes coding for the large subunit of Mo-nitrogenase and hupS gene coding for the small subunit of uptake hydrogenase. Hydrogen productions under the same growth conditions were also evaluated. Increasing concentrations of the molybdenum and iron stimulated hydrogen production and the highest total hydrogen accumulation was achieved in 16.51 mu M sodium molyb...

Citation Formats

S. Akkose, U. Gündüz, M. Yucel, and I. Eroglu, “Effects of ammonium ion, acetate and aerobic conditions on hydrogen production and expression levels of nitrogenase genes in Rhodobacter sphaeroides OU001,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 8818–8827, 2009, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32259.