Effect of salt stress on antioxidant defense systems of sensitive and resistance cultivars of lentil(Lens culinaris M.)

Cicerali, Işın Nur
In this study, two lentil cultivars (Lens culinaris, Medik.) (ILL5582-salt tolerant and ILL590) were characterized and compared due to their NaCl susceptibility and antioxidant mechanism was examined under laboratory conditions. Physiological parameters such as wet-dry weight, root-shoot lengths, cell membrane stability, lipid peroxidation in terms of malondialdehyde (MDA), H2O2, proline contents were determined. The activities of antioxidant enzymes such as superoxide dismutase (SOD: EC, catalase (CAT: EC, ascorbate peroxidase (APX: EC and glutathione reductase (GR: EC were examined and analyzed in 14 days old plant seedlings after 9 days of normal growth and 5 days of 100mM and 200mM NaCl stress conditions. Shoot-root length and wet-dry weight percent decrease were more in ILL590. Especially shoot tissues were affected more from the stress conditions when compared to root tissues. ii According to malondialdehyde (MDA) content and membrane stability results, lipid peoxidation was higher in ILL590 and significant increases were observed in shoot tissues. Proline concentration showed a remarkable increase in salt concentration dependent manner. Higher concentrations of proline in ILL5582 might be the reason of higher salt tolerance when compared to ILL590. Among the antioxidant enzymes SOD was the one which showed highest activity increase. At organ level roots showed highest activity when compared to leaves. In the organelle higher activity percent contribution was achieved by cytosolic Cu/ZnSOD isozyme. Higher percent increase of this isozyme was observed in ILL5582. This might be one of the tolerance mechanisms that get activated against NaCl stress. APX activity showed similar alterations in both cultivars. In leaf tissues significant increase was observed but in root tissues ascorbate peroxidase activity did not change


Evaluation of photosynthetic performance of wheat cultivars exposed to boron toxicity by the JIP fluorescence test
Oz, M. T.; TURAN, ÖZLEM; Kayihan, C.; EYİDOĞAN, FÜSUN; EKMEKÇİ, YASEMİN; YÜCEL, MUSTAFA; Öktem, Hüseyin Avni (Institute of Experimental Botany, 2014-12-01)
The changes in growth and photosynthetic performance of two wheat (Triticum aestivum L.) cultivars (Bolal-2973 and Atay-85) differing in their sensitivity to boron (B) toxicity were investigated under toxic B conditions. Eight-day old seedlings were exposed to highly toxic B concentrations (5, 7.5, and 10 mM H3BO3) for 5 and 9 days. Fast chlorophyll a fluorescence kinetics was determined and analysed using JIP test. Growth parameters, tissue B contents, and membrane damage were measured at two stress durati...
Expression profiling in response to ascochyta rabiei inoculations in chickpea
Avcıoğlu Dündar, Banu; Akkaya, Mahinur S.; Department of Biotechnology (2008)
In this study, it was aimed to identify chickpea (Cicer arietinum) genes or gene fragments expressed upon Ascochyta rabiei infection using a tolerant chickpea cultivar ILC195 and fungal isolates with varying level of pathogenicity. PCR amplification of resistance gene analogs (RGA) and disease related genes, and mRNA differential display reverse transcription (DDRT) were used to get these expressed gene fragments in chickpea. The constitutively or differentially expressed PCR product fragments were cloned a...
Effect of drought and salt stresses on antioxidant defense system and physiology of lentil (lens culinaris m.) seedlings
Ercan, Oya; Öktem, Hüseyin Avni; Department of Biology (2008)
In this study, 14 days old lentil seedlings (Lens culinaris Medik cv. Sultan), which were subjected to 7 days of drought (20% PEG 6000), and salt (150 mM NaCl ) stress , were examined in a comparative manner for the effects of drought and salt stress treatments. In shoot and root tissues physiological parameters such as wet-dry weight, relative water content, root-shoot lengths, membrane electrolyte leakage, and lipid peroxidation in terms of malondialdehyde (MDA) were determined. H2O2 content, proline accu...
Effect of suppressive DNA on CpG-induced immune activation.
Yamada, H; Gursel, I; Takeshita, F; Conover, J; Ishii, KJ; Gürsel, Mayda; Takeshita, S; Klinman, DM (The American Association of Immunologists, 2002-11-15)
Bacterial DNA and synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG motifs stimulate a strong innate immune response. This stimulation can be abrogated by either removing the CpG DNA or adding inhibitory/suppressive motifs. Suppression is dominant over stimulation and is specific for CpG-induced immune responses (having no effect on LPS- or Con A-induced activation). Individual cells noncompetitively internalize both stimulatory and suppressive ODN. Studies using ODN composed of both stimula...
Cell wall uronic acid concentrations of resistant and sensitive cultivars of wheat and barley under boron toxicity
Mahboobi, H; Yucel, M; Öktem, Hüseyin Avni (Informa UK Limited, 2001-01-01)
In this study, changes in cell wall uronic acid concentrations of boron (B)-tolerant and B-sensitive cultivars; of wheat and barley were analyzed in control (plants that were grown on water and received no B) and B-treated plants. Ten-day-old seedlings of wheat (Triticum aestivum L. 'Atay' (B-sensitive) and 'Bolal' (B-tolerant)) and barley (Hordeum vulgare L. 'Hamidiye' (B-sensitive) and 'Anadolu' (B-tolerant)) were grown in H3BO3 solution (10 mM final concentration) or water for 3, 5, or 7 days. For determ...
Citation Formats
I. N. Cicerali, “Effect of salt stress on antioxidant defense systems of sensitive and resistance cultivars of lentil(Lens culinaris M.),” M.S. - Master of Science, Middle East Technical University, 2004.