Retinal pigment epithelium cell culture on surface modified poly(hydroxybutyrate-co-hydroxyvalerate) thin films

Date

2003-01-01

Author

Tezcaner, Ayşen
Hasırcı, Vasıf Nejat

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

107
views

0
downloads

There is currently no effective treatment for the retinal disorders caused by retinal pigment epithelium (RPE) degeneration. Transplantation of allografts is the main strategy towards correction of this malady. Tissue engineering could offer hope and involve the use of biodegradable polymeric templates to replace diseased or lost RPE. In this study PHBV8 film was chosen as a temporary substrate for growing retinal pigment epithelium cells as an organized monolayer before their subretinal transplantation. The surface of the PHBV8 film was rendered hydrophilic by oxygen plasma treatment to increase the reattachment of D407 cells on the film surface. Power and duration was changed, from 50W, 10 min to 100 W, 20 min during plasma treatment. The effect of these two parameters on surface hydrophilicity, morphology, topography, surface composition of PHBV8 thin films was studied using AFM, SEM, and phase contrast microscopy. The effect of changes in surface characteristics on cell reattachment, spreading and cell growth rate was investigated. It was found that as the treatment level was increased the surface hydrophilicity increased and roughness was decreased probably due to ablation. The PHBV8 film treated with 100 W 10 min was found to be the most suitable for 24 It reattachment of D407 cells. The cells were also grown to confluency as an organized monolayer suggesting PHBV8 film as a potential temporary substrate for subretinal transplantation to replace diseased or damaged retinal pigment epithelium.

Subject Keywords

Retinal pigment epithelium, Cell culture, Tissue engineering, PHBV, Biodegradable

URI

https://hdl.handle.net/11511/32319

Journal

Biomaterials

DOI

https://doi.org/10.1016/s0142-9612(03)00302-8

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Retinal vessel segmentation using convolutional neural networks Ulusoy, İlkay (2018-05-05) Retinal vessel segmentation and extracting features such as tortuosity, width, length related to those vessels can be used in diagnosis, treatment and screening of many diseases such as retinopathy of prematurity, hypertension and diabetes. Therefore, automatic segmentation of vessels by computers will make the analysis of those diseases easier and will help during the screening, diagnosis and treatment processes. In this study, a solution based on convolutional neural networks (CNN) is proposed for automat...
Biodegradable poly (ester-urethane) scaffolds for bone tissue engineering Kızıltay, Aysel; Hasırcı, Nesrin; Department of Biotechnology (2011) During last decade, polyurethanes (PUs) which are able to degrade into harmless molecules upon implantation have received a significant level of attention as a biomaterial in tissue engineering applications. Many studies are focused especially on development of PUs based on amino acid derivatives; however, there are only few applications of amino acid based PUs in tissue engineering. In this study, a biocompatible and biodegradable thermoplastic poly(ester-urethane) (PEU) based on L-lysine diisocyanate (LDI...
Influence of structural length-scale variations on azimuth-resolved light scattering patterns of inhomogeneous cell models Arifler, Dizem; Guillaud, Martial (2015-06-24) Optical scattering provides an intrinsic contrast mechanism for the diagnosis of early precancerous changes in tissues. There have been a multitude of numerical studies targeted at delineating the relationship between cancer-related alterations in morphology and internal structure of cells and the resulting changes in their optical scattering properties. Despite these efforts, we still need to further our understanding of inherent scattering signatures that can be linked to precancer progression. As such, c...
Nickel titanium alloys as orthodontic archwires: A narrative review Uysal, Idil; Yilmaz, Bengi; Atilla, Aykan Onur; Evis, Zafer (2022-12-01) Nickel-titanium (NiTi) archwires have been widely used in orthodontic treatments at the aligning and leveling step which is the initial stage of the treatment. This review presents the concepts related to NiTi archwires from the materials science and engineering perspective. Analysis of the research profile showed that mechanical properties as a research topic constituted 25.9 % of the research articles published in last two decades. The effects of processing parameters, stress and temperature on phase tran...
Nanocrystal Encoded FluorescentMicrobeads for Proteomics AntibodyProfiling and Diagnostics ofAutoimmune Diseases Sukhanova, Alyona; Susha , Andrei; Bek, Alpan; Mayilo, Sergiy; Rogach , Andrey; Feldmann, Jochen; Oleinikov, Vladimir; Reveil, Brigitte; Donvito, Beatrice; Cohen, Jacques; Nabiev, İgor (2007-01-01) The first application of nanocrystal (NC)-encoded microbeads to clinical proteomics is demonstrated by multiplexed detection of circulating autoantibodies, markers of systemic sclerosis. Two-color complexes, consisting of NC-encoded, antigen-covered beads, anti-antigen antibody or clinical serum samples, and dye-tagged detecting antibodies, were observed using flow cytometry assays and on the surface of single beads. The results of flow cytometry assays correlated with the ELISA technique and provided clear...

Citation Formats

A. Tezcaner and V. N. Hasırcı, “Retinal pigment epithelium cell culture on surface modified poly(hydroxybutyrate-co-hydroxyvalerate) thin films,” Biomaterials, pp. 4573–4583, 2003, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32319.