An in vivo study on the effect of scaffold geometry and growth factor release on the healing of bone defects

Date

2013-09-01

Author

Yilgor, P.
Yilmaz, G.
Onal, M. B.
Solmaz, I.
Gundogdu, S.
Keskil, S.
Sousa, R. A.
Reis, R. L.
Hasırcı, Nesrin
Hasırcı, Vasıf Nejat

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

127
views

0
downloads

The hypothesis of this study was that the extent of bone regeneration could be enhanced by using scaffolds with appropriate geometry, and that such an effect could be further increased by mimicking the natural timing of appearance of bone morphogenetic proteins BMP-2 and BMP-7 after fracture. Bioplotted poly(epsilon-caprolactone) (PCL) disks with four different fibre organizations were used to study the effect of 3D scaffold architecture on the healing of bone defects in a rat pelvis model. Moreover, one PCL construct was further modified by introducing a nanoparticulate sequential BMP-2/BMP-7 delivery system into this scaffold. Scaffolds and functionalized construct along with free nanocapsules were implanted using a rat iliac crest defect model. Six weeks post-implantation, the defects were evaluated by CT scan and histology. Analysis revealed that the basic architecture, having the highest pore volume for tissue ingrowth, presented the highest bone formation as determined by the bone mineral density (BMD) within the defect (144.2 +/- 7.1); about four-fold higher than that of the empty defect (34.9 +/- 10.7). It also showed the highest histological analysis scores with a high amount of bone formation within the defect, within the scaffold pores and along the outer surfaces of the scaffold. The basic scaffold carrying the BMP-2/BMP-7 delivery system showed significantly higher bone formation than the growth factor-free basic scaffold at 6weeks (BMD 206.8 +/- 15.7). Histological analysis also revealed new bone formation in close to or in direct contact with the construct interface. This study indicates the importance of open and interconnecting pore geometry on the better healing of bone defects, and that this effect could be further increased by supplying growth factors, as is the case in nature. Copyright (c) 2012 John Wiley & Sons, Ltd.

Subject Keywords

Bone regeneration, Scaffold, Bone morphogenetic protein, Controlled release, 3D plotting

URI

https://hdl.handle.net/11511/32333

Journal

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE

DOI

https://doi.org/10.1002/term.1456

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

An Experimental study of mechanical properties of non enzymatically glycated bovine femur cortical bone Fındıkoğlu, Gülin; Evis, Zafer; Department of Engineering Sciences (2012) The aim of this study is to investigate the deterioration in mechanical integrity of the collagen network in bovine bone with aging, which are related to fracture toughness. Age-related changes in collagen molecular structures formed by non-enzymatic glycation were examined and indentation fracture technique was used as a method for measuring the microstructural toughness of cortical bone. Microcrack propagation characteristics of bone for fragility were also studied. Young and old group of bovine cortical ...
Investigation of the effect of ghrelin on bone fracture healing in rats Erener, Tamer; CERİTOĞLU, KUBİLAY UĞURCAN; AKTEKİN, CEM NURİ; Dalgic, Ali Deniz; Keskin, Dilek; GENECİ, FERHAT; Ocak, Mert; Bilecenoğlu, Burak; Hücümenoğlu, Sema; Çaydere, Muzaffer; Şeneş, Mehmet; Sezgin, Özge (2021-01-01) Ghrelin is known to have effects on proliferation and differentiation of osteoblasts and improvement of bone mineral density in rats. However, no experimental research on ghrelin's effects on fracture healing has been reported. In this context, the effect of ghrelin on the union of femoral shaft fractures was examined in this study by evaluating whether ghrelin will directly contribute to fracture healing. Forty male Wistar-Albino rats were divided into two groups as control and experimental (ghrelin treate...
Differential expressions and functions of phosphodiesterase enzymes in different regions of the rat heart DERİCİ, MEHMET KÜRŞAT; SADİ, GÖKHAN; Cenik, Basar; Güray, Nülüfer Tülün; DEMİREL YILMAZ, EMİNE (Elsevier BV, 2019-02-05) Phosphodiesterase enzymes (PDEs) are responsible for the adjustment of cyclic nucleotide levels. Alterations in PDE expressions in different tissues cause conflicts between functional and clinical effects of PDE inhibitors. Therefore, the aim of this study was to investigate the gene and protein expressions and the functional role of PDEs in atrium and ventricle of rat heart The expressions of PDEs were examined in cardiac intact tissues and enzymatically isolated cells. The effects of PDE1-5 inhibitors (vi...
In-vivo directed evolution of galactose oxidase by stationary phase adaptive mutations and phylogenetic analysis of error-prone polymerases Öreroğlu, Ayla; Ögel, Zümrüt Begüm; Department of Food Engineering (2007) In this study, the novel idea of in-vivo directed evolution was applied in order to achieve variants of the enzyme galactose oxidase with increased activity. This procedure was done under starvation conditions in Escherichia coli BL21 Star (DE3). Previous studies have been carried out in order to improve the activity of this enzyme using directed evolution methods. In this study, the same idea was used in-vivo, during stationary phase adaptive mutations inside the host organism, hence called in-vivo directe...
The molecular investigation of the effects of simvastatin, a cholesterol reducing drug, on different rat skeletal muscle tissues Şimşek Özek, Nihal; Severcan, Feride; Department of Biology (2007) In the present study Fourier Transform Infrared (FTIR) and Attenuated Total Reflectance FTIR (ATR-FTIR) Spectroscopy were used to examine the effects of simvastatin on structure, composition and function of macromolecules of three different rat skeletal muscles EDL (Extensor Digitorium Longus), DIA (Diaphragm) and SOL (Soleus) containing different amount of slow and fast twitch fibers, at molecular level. Simvastatin, a lipophilic statin, is widely used in the treatment of hypercholesterolemia and cardiovas...

Citation Formats

P. Yilgor et al., “An in vivo study on the effect of scaffold geometry and growth factor release on the healing of bone defects,” JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, pp. 687–696, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32333.