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In vivo response to biodegradable controlled antibiotic release systems
Date
2001-05-01
Author
Korkusuz, F
Korkusuz, P
Eksioglu, F
Gursel, I
Hasırcı, Vasıf Nejat
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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In this study, the major goal was to evaluate in vitro and in vivo findings by macroscopy, radiology, and histology to determine the effectiveness of therapy of experimental implant-related osteomyelitis with antibiotic carrier rods constructed of microbial polyesters. The polymers used were poly(3-hydroxybutyrate-co-4-hydroxyvalerate) [P(3HB-co-4-HB)] and poly(3-hydroxybutyrate-co-3-hydroxy-valerate) [P(3-HB-co-3-HV)]. Both the Sulperazone(R) and the Duocid(R)-P(3-HB-co-4-HB) rods with a drug to polymer ratio of 1:1 (w/w) were effective in treating the bone infection that was experimentally initiated by inoculation of a hemolytic strain of Staphylococcus aureus (coagulase positive; phage type 52/52b) together with metal implants into the medullary area of rabbit tibia. Macroscopical data revealed that the effectiveness of therapy was apparent at week 6 for all categories tested. Radiological findings with Duocid(R)- and Sulperazone(R)-loaded P(3-HB-co-4-HB) rods improved significantly when judged by changes in periosteal elevation, widening of bone shaft, new bone formation, and soft-tissue deformation after 6 weeks of implantation. Histologically the signs of infection were found to subside by weeks 3 and 6. inflammatory cells were replaced with bone-forming cells upon treatment with Sulperazone(R)-P(3-HB-co-4-HB) and Duocid(R)-P(3-HB-co-4-HB). Osteoblastic activity was prominent. Intramedullary inflammation, although still present, started to be replaced by fibrous or bony tissue. Histological findings presented the subsidence of infection. In summary, the antibiotic-loaded biopolymeric rods appeared to have potential as a new controlled-release system for the treatment of implant related osteomyelitis and chronic osteomyelitis. (C) 2001 John Wiley & Sons, Inc.
Subject Keywords
Osteomyelitis
,
Sustained release
,
Local antiinfective agents
,
Biodegradable delivery systems
,
Polymers
,
Poly(hydroxybutyrate-co-hydroxyvalerate)
URI
https://hdl.handle.net/11511/30224
Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
DOI
https://doi.org/10.1002/1097-4636(200105)55:2<217::aid-jbm1008>3.3.co;2-p
Collections
Graduate School of Natural and Applied Sciences, Article
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In vivo application of biodegradable controlled antibiotic release systems for the treatment of implant-related osteomyelitis
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F. Korkusuz, P. Korkusuz, F. Eksioglu, I. Gursel, and V. N. Hasırcı, “In vivo response to biodegradable controlled antibiotic release systems,”
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
, pp. 217–228, 2001, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30224.