Bone tissue engineering on patterned collagen films: an in vitro study

Ber, S
Kose, GT
Hasırcı, Vasıf Nejat
This study aimed at guiding osteoblast cells from rat bone marrow on chemically modified and patterned collagen films to study the influence of pattern, on cell guidance. The films were stabilized using different treatment methods including crosslinking with carbodiimide (EDC) and glutaraldehyde, dehydrothermal treatment (DHT), and deposition of calcium phosphate on the collagen membrane.


Bone generation on PHBV matrices: an in vitro study
Kose, GT; Korkusuz, F; Korkusuz, P; Purali, N; Ozkul, A; Hasırcı, Vasıf Nejat (2003-12-01)
Bone formation was investigated in vitro by culturing rat marrow stromal osteoblasts in biodegradable, macroporous poly(3-hydroxybutyric acid-co-3-bydroxyvaleric acid) (PHBV) matrices over a period of 60 days. Foams were prepared after solvent evaporation and solute leaching. PHBV solutions with different concentrations were prepared in chloroform: dichloromethane (1:2, v/v). In order to create a matrix with high porosity and uniform pore sizes, sieved sucrose crystals (300-500 mum) were used. PHBV foams we...
Bone tissue generation on biodegradable polymeric scaffolds
Torun Köse, Gamze; Hasırcı, Vasıf Nejat; Korkusuz, Feza; Department of Biotechnology (2002)
In the present study, tissue engineered bone was produced on calcium phosphate loaded collagen and PHBV8 matrices. Osteoblasts isolated from rat bone marrow were characterized by light microscopy, alkaline phosphatase (ALP) activity, osteocalcin determination assay, and Western blots for integrin. Population doubling time of the cells was found as 50 ± 2 h at 37 °C in a CO2 incubator. PHBV8 foams iiiwere treated with rf-oxygen plasma to modify their surface chemistry and hydrophilicity to increase the reatt...
Tissue engineering of bone on micropatterned biodegradable polyester films
Kenar, H; Kose, GT; Hasırcı, Vasıf Nejat (2006-02-01)
In this study, the effect of cell alignment on proliferation and phenotype expression of rat bone marrow derived osteoblasts on micropatterned (MP) PHBV and P(L/D,L)LA films with 27 mu m wide parallel microgrooves was investigated. Immobilization of fibrinogen (Fb) on film surface by adsorption increased hydrophilicity, while covalent immobilization decreased it. Amount of Fb immobilized was significantly higher upon covalent bonding (153.1 +/- 42.4 mu g Fb/cm(2)) than when adsorbed (10.0 +/- 3.3 mu g Fb/cm...
Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress
Zorlutuna, P.; Rong, Z.; Vadgama, P.; Hasırcı, Vasıf Nejat (2009-09-01)
In this study, nanopatterned crosslinked films of collagen Type I were seeded with human microvascular endothelial cells and tested for their suitability for vascular tissue engineering. Since the films will be rolled into tubes with concentric layers of collagen, nutrient transfer through the collagen films is quite crucial. Molecular diffusivity through the collagen films, cell viability, cell proliferation and cell retention following shear stress were studied. Cells were seeded onto linearly nanogrooved...
Cartilage tissue engineering on macroporous scaffolds using human tooth germ stem cells
Koyuncu, A. C. Calikoglu; Pekozer, G. Gurel; Ramazanoğlu, Mehmet Kerim; KÖSE, GAMZE; Hasırcı, Vasıf Nejat (2017-03-01)
The main objective was to study cartilage regeneration through differentiation of human tooth germ stem cells (HTGSCs) into chondrocytes on different three-dimensional (3D) scaffolds (PCL, PLLA and PCL-PLLA). Scaffold topographies were studied by scanning electron microscopy and it was found that the scaffolds had interconnected macroporous structures. HTGSCs were isolated from impacted third molar tooth germs of young adult patients and grown for 3 weeks on the scaffolds in chondrogenic differentiation med...
Citation Formats
S. Ber, G. Kose, and V. N. Hasırcı, “Bone tissue engineering on patterned collagen films: an in vitro study,” BIOMATERIALS, pp. 1977–1986, 2005, Accessed: 00, 2020. [Online]. Available: