Cartilage tissue engineering on macroporous scaffolds using human tooth germ stem cells

Date

2017-03-01

Author

Koyuncu, A. C. Calikoglu
Pekozer, G. Gurel
Ramazanoğlu, Mehmet Kerim
KÖSE, GAMZE
Hasırcı, Vasıf Nejat

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

138
views

0
downloads

The main objective was to study cartilage regeneration through differentiation of human tooth germ stem cells (HTGSCs) into chondrocytes on different three-dimensional (3D) scaffolds (PCL, PLLA and PCL-PLLA). Scaffold topographies were studied by scanning electron microscopy and it was found that the scaffolds had interconnected macroporous structures. HTGSCs were isolated from impacted third molar tooth germs of young adult patients and grown for 3 weeks on the scaffolds in chondrogenic differentiation medium. Cell proliferation on the scaffolds was determined by MTS assay and it was observed that all scaffolds supported cell proliferation. Immunostaining was carried out for morphological and differentiation analyses. Immunohistochemical analyses revealed that the cells attached onto the scaffolds and deposited cartilage-specific extracellular matrix (ECM). Real-time PCR was performed to determine the expression levels of cartilage-specific genes. After 21 days of incubation in cartilage differentiation medium, expression of collagen type II increased only in the cells seeded onto PCL-PLLA blend scaffolds. Similarly, aggrecan expression was the highest on PCL-PLLA scaffolds after 3weeks. These results suggest that all the scaffolds, and especially PCL-PLLA, were suitable for chondrogenic differentiation of HTGSCs. Copyright (C) 2015 John Wiley & Sons, Ltd.

Subject Keywords

Cartilage tissue engineering, Human tooth germ stem cells, Cell differentiation, Polyester scaffolds, Polycaprolactone, Poly(L-lactide)

URI

https://hdl.handle.net/11511/31059

Journal

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE

DOI

https://doi.org/10.1002/term.1975

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Characterization and identification of human mesenchymal stem cells at molecular level Aksoy, Ceren; Severcan, Feride; Çetinkaya, Duygu Uçkan; Department of Biotechnology (2012) Bone marrow mesenchymal stem cells (BM-MSCs) are pluripotent cells that can differentiate into a variety of non-hematopoietic tissues. They also maintain healthy heamatopoiesis by providing supportive cellular microenvironment into BM. In this thesis, MSCs were characterized in terms of their morphological, immunophenotypical and differentiation properties. Then, they were examined by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy together with hierarchical clustering, and FT...
Effect of double growth factor release on cartilage tissue engineering Ertan, Ayse Burcu; Yilgor, Pinar; Bayyurt, Banu; Calikoglu, Ayse Ceren; Kaspar, Cigdem; Kök, Fatma Neşe; KÖSE, GAMZE; Hasırcı, Vasıf Nejat (2013-02-01) The effects of double release of insulin-like growth factor I (IGF-I) and growth factor 1 (TGF1) from nanoparticles on the growth of bone marrow mesenchymal stem cells and their differentiation into cartilage cells were studied on PLGA scaffolds. The release was achieved by using nanoparticles of poly(lactic acid-co-glycolic acid) (PLGA) and poly(N-isopropylacrylamide) (PNIPAM) carrying IGF-I and TGF1, respectively. On tissue culture polystyrene (TCPS), TGF-1 released from PNIPAM nanoparticles was found to ...
Growth factor loaded silk fibroin/PEGDMA hydrogels for articular cartilage tissue engineering Fathi Achachelouei, Milad; Tezcaner, Ayşen; Engin Vrana, Nihal; Department of Biomedical Engineering (2018) The aim of this study was to develop bFGF and TGF-β1 loaded polymeric nanoparticles (PNPs) and dental pulp stem cells (DPSCs) containing dimethacrylated poly(ethylene glycol) (PEGDMA)/silk fibroin hydrogels as scaffolds for regeneration of the cartilage tissue. Poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) were prepared with double emulsion-solvent evaporation technique. The effect of different excipients (poly(2-ethyl-2-oxazoline) (PetOx), heparin and Kolliphor P 188) on the entrapment efficienc...
Collagen scaffolds with in situ-grown calcium phosphate for osteogenic differentiation of Wharton's jelly and menstrual blood stem cells Karadas, Ozge; Yucel, Deniz; Kenar, Halime; Kose, Gamze Torun; Hasırcı, Vasıf Nejat (2014-07-01) The aim of this research was to investigate the osteogenic differentiation potential of non-invasively obtained human stem cells on collagen nanocomposite scaffolds with in situ-grown calcium phosphate crystals. The foams had 70% porosity and pore sizes varying in the range 50-200 mu m. The elastic modulus and compressive strength of the calcium phosphate containing collagen scaffolds were determined to be 234.5 kPa and 127.1 kPa, respectively, prior to in vitro studies. Mesenchymal stem cells (MSCs) obtain...
Bone tissue engineering on patterned collagen films: an in vitro study Ber, S; Kose, GT; Hasırcı, Vasıf Nejat (2005-05-01) This study aimed at guiding osteoblast cells from rat bone marrow on chemically modified and patterned collagen films to study the influence of pattern, on cell guidance. The films were stabilized using different treatment methods including crosslinking with carbodiimide (EDC) and glutaraldehyde, dehydrothermal treatment (DHT), and deposition of calcium phosphate on the collagen membrane.

Citation Formats

A. C. C. Koyuncu, G. G. Pekozer, M. K. Ramazanoğlu, G. KÖSE, and V. N. Hasırcı, “Cartilage tissue engineering on macroporous scaffolds using human tooth germ stem cells,” JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, pp. 765–777, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31059.