Cartilage tissue engineering on macroporous scaffolds using human tooth germ stem cells

Date

2017-03-01

Author

Koyuncu, A. C. Calikoglu
Pekozer, G. Gurel
Ramazanoğlu, Mehmet Kerim
KÖSE, GAMZE
Hasırcı, Vasıf Nejat

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The main objective was to study cartilage regeneration through differentiation of human tooth germ stem cells (HTGSCs) into chondrocytes on different three-dimensional (3D) scaffolds (PCL, PLLA and PCL-PLLA). Scaffold topographies were studied by scanning electron microscopy and it was found that the scaffolds had interconnected macroporous structures. HTGSCs were isolated from impacted third molar tooth germs of young adult patients and grown for 3 weeks on the scaffolds in chondrogenic differentiation medium. Cell proliferation on the scaffolds was determined by MTS assay and it was observed that all scaffolds supported cell proliferation. Immunostaining was carried out for morphological and differentiation analyses. Immunohistochemical analyses revealed that the cells attached onto the scaffolds and deposited cartilage-specific extracellular matrix (ECM). Real-time PCR was performed to determine the expression levels of cartilage-specific genes. After 21 days of incubation in cartilage differentiation medium, expression of collagen type II increased only in the cells seeded onto PCL-PLLA blend scaffolds. Similarly, aggrecan expression was the highest on PCL-PLLA scaffolds after 3weeks. These results suggest that all the scaffolds, and especially PCL-PLLA, were suitable for chondrogenic differentiation of HTGSCs. Copyright (C) 2015 John Wiley & Sons, Ltd.

Subject Keywords

Cartilage tissue engineering, Human tooth germ stem cells, Cell differentiation, Polyester scaffolds, Polycaprolactone, Poly(L-lactide)

URI

https://hdl.handle.net/11511/31059

Journal

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE

DOI

https://doi.org/10.1002/term.1975

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

A. C. C. Koyuncu, G. G. Pekozer, M. K. Ramazanoğlu, G. KÖSE, and V. N. Hasırcı, “Cartilage tissue engineering on macroporous scaffolds using human tooth germ stem cells,” JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, pp. 765–777, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31059.