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Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress
Date
2009-09-01
Author
Zorlutuna, P.
Rong, Z.
Vadgama, P.
Hasırcı, Vasıf Nejat
Metadata
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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In this study, nanopatterned crosslinked films of collagen Type I were seeded with human microvascular endothelial cells and tested for their suitability for vascular tissue engineering. Since the films will be rolled into tubes with concentric layers of collagen, nutrient transfer through the collagen films is quite crucial. Molecular diffusivity through the collagen films, cell viability, cell proliferation and cell retention following shear stress were studied. Cells were seeded onto linearly nanogrooved films (groove widths of 332.5, 500 and 650 nm), with the grooves aligned in the direction of flow. The nanopatterns did not affect cell proliferation or initial cell alignment; however, they significantly affected cell retention under fluid flow. While cell retention on unpatterned films was 35 +/- 10%, it was 75 +/- 4% on 332.5 nm patterned films and even higher, 91 +/- 5%, on 650 nm patterned films. The films were found to have diffusion coefficients of ca. 10(-6) cm(2) s(-1) for O-2 and 4-acetaminophenol, which is comparable to that observed in natural tissues. This constitutes another positive asset of these films for consideration as a scaffold material for vascular tissue engineering. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Subject Keywords
Shear stress
,
HMEC
,
Collagen
,
Nanopattern
,
Vascular tissue engineering
URI
https://hdl.handle.net/11511/30006
Journal
ACTA BIOMATERIALIA
DOI
https://doi.org/10.1016/j.actbio.2009.03.027
Collections
Graduate School of Natural and Applied Sciences, Article
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P. Zorlutuna, Z. Rong, P. Vadgama, and V. N. Hasırcı, “Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress,”
ACTA BIOMATERIALIA
, pp. 2451–2459, 2009, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30006.