Expression Analysis of TaNAC69-1 and TtNAMB-2, Wheat NAC Family Transcription Factor Genes Under Abiotic Stress Conditions in Durum Wheat (Triticum turgidum)

2012-10-01
Baloglu, Mehmet Cengiz
Oz, Mehmet Tufan
Öktem, Hüseyin Avni
Yücel, Ayşe Meral
NAC-type plant-specific transcription factor genes encode proteins that play important roles in abiotic stress responses, as well as regulation of plant development. In the current study, expression profiles of wheat NAC-type transcription factor genes, TaNAC69-1 and TtNAMB-2, were examined under drought, salt, cold, and heat stress conditions in wheat. Based on reverse transcription quantitative PCR results, TaNAC69-1 was strongly expressed under drought, salinity, and high-temperature stress conditions. Compared to control samples, a quick response at the transcription level of TaNAC69-1 was observed after 3 h of salt treatment with a ninefold upregulation. Highest level of expression was observed at 24 and 48 h posttreatment under heat and salinity treatments, respectively. Meanwhile, expression of TtNAMB-2 was significantly induced by salt and low-temperature stresses. Salt treatment induced expression of TtNAMB-2 and caused a 13-fold increase in transcript copy numbers at 48 h posttreatment. Examination of expression changes under abiotic stresses may provide important information for understanding roles of TaNAC69-1 and TtNAMB-2 genes which might be involved in response to environmental stresses.
PLANT MOLECULAR BIOLOGY REPORTER

Suggestions

Expression and activity analyses of industrially important extracellular enzymes produced by a bacilysin knock-out mutant of Bacillus Subtilis
Aytekin, Samet; Özcengiz, Gülay; Okay, Sezer; Department of Molecular Biology and Genetics (2018)
Bacilysin, the smallest peptide antibiotic known to date, is produced non-ribosomally by Bacillus subtilis by the collective actions of seven proteins transcribed from bacABCDEF operon and bacG gene. Bacilysin is a two-amino acid peptide composed of L-alanine and a modified amino acid, L-anticapsin. Bacilysin biosynthesis was shown to be strongly regulated by quorum sensing through the actions of global regulator proteins including Spo0K, Spo0H, Spo0A, ComQ/ComX, ComP/ComA as well as several Phr proteins, O...
Functional Constraints on Replacing an Essential Gene with Its Ancient and Modern Homologs
Kacar, Betul; Garmendia, Eva; Tunçbağ, Nurcan; Andersson, Dan I.; Hughes, Diarmaid (2017-07-01)
Genes encoding proteins that carry out essential informational tasks in the cell, in particular where multiple interaction partners are involved, are less likely to be transferable to a foreign organism. Here, we investigated the constraints on transfer of a gene encoding a highly conserved informational protein, translation elongation factor Tu (EF-Tu), by systematically replacing the endogenous tufA gene in the Escherichia coli genome with its extant and ancestral homologs. The extant homologs represented...
Transcriptional regulatory proteins in central carbon metabolism ofPichia pastorisandSaccharomyces cerevisiae
Kalender, Ozge; Çalık, Pınar (2020-09-01)
System-wide interactions in living cells and discovery of the diverse roles of transcriptional regulatory proteins that are mediator proteins with catalytic domains and regulatory subunits and transcription factors in the cellular pathways have become crucial for understanding the cellular response to environmental conditions. This review provides information for future metabolic engineering strategies through analyses on the highly interconnected regulatory networks inSaccharomyces cerevisiaeandPichia past...
Inference of Gene Regulatory Networks Via Multiple Data Sources and a Recommendation Method
Ozsoy, Makbule Gulcin; Polat, Faruk; Alhajj, Reda (2015-11-12)
Gene regulatory networks (GRNs) are composed of biological components, including genes, proteins and metabolites, and their interactions. In general, computational methods are used to infer the connections among these components. However, computational methods should take into account the general features of the GRNs, which are sparseness, scale-free topology, modularity and structure of the inferred networks. In this work, observing the common aspects between recommendation systems and GRNs, we decided to ...
Expression System for Recombinant Human Growth Hormone Production from Bacillus subtilis
ÖZDAMAR, HASAN TUNÇER; Sentuerk, Birguel; Yilmaz, Oezge Deniz; Calik, Guezide; Celik, Eda; Çalık, Pınar (2009-01-01)
We demonstrate for the first time, an expression system mimicking serine alkaline protease synthesis and secretion, producing native form of human growth hormone (hGH) from Bacillus subtilis. A hybrid-gene of two DNA fragments, i.e., signal (pre-) DNA sequence of B. licheniformis serine alkaline protease gene (subC) and cDNA encoding hGH, were cloned into pMK4 and expressed under deg-promoter in B. subtilis. Recombinant-hGH (rhGH) produced by B. subtilis carrying pMK4::pre(subC)::hGH was secreted. N-termina...
Citation Formats
M. C. Baloglu, M. T. Oz, H. A. Öktem, and A. M. Yücel, “Expression Analysis of TaNAC69-1 and TtNAMB-2, Wheat NAC Family Transcription Factor Genes Under Abiotic Stress Conditions in Durum Wheat (Triticum turgidum),” PLANT MOLECULAR BIOLOGY REPORTER, pp. 1246–1252, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32615.