A novel GeIMA-pHEMA hydrogel nerve guide for the treatment of peripheral nerve damages

Yucel, Deniz
Hasırcı, Vasıf Nejat
Damage to the nervous system due to age, diseases or trauma may inhibit signal transfer along the nervous system. Nerve guides are used to treat these injuries by bridging the proximal and the distal end together. The design of the guide is very important for the reconnection of the severed axons. Methacrylated gelatin-poly(2-hydroxyethylmethacrylate) (GeIMA-pHEMA) hydrogel was produced as the outer part of the nerve guide. pHEMA was added in various amounts into GeIMA and increased the mechanical strength which is needed for the suturability of the guide. Porosity (15-70%), pore size (10-35 mu m), water content (42-92%), and mechanical strength (65-710 kPa) of GeIMA-pHEMA hydrogels were found to be suitable for nerve tissue engineering applications. Schwann cells attached and proliferated on GeIMA, GeIMA-pHEMA (5:5), and pHEMA hydrogels. Providing guidance is very important in the development of a nerve guide due to the anisotropic nature of the nerve tissue. Therefore, gelatin-poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) aligned fiber mats were used inside of the nerve guide. High degree of alignment with low deviation (r) of this mats provided PC12 cell alignment throughout the fibers. Combination of GeIMA-pHEMA (5:5) hydrogel and gelatin-PHBV aligned mat would provide an ideal nerve guide for the treatment of peripheral nerve damages. (C) 2018 Published by Elsevier B.V.


A Bio and synthetic polymer based nerve guide tested under in vitro and in vivo conditions
Dursun Usal, Tuğba; Hasırcı, Vasıf Nejat; Department of Biotechnology (2019)
Damages to the peripheral nervous system due to age, diseases or trauma may lead to gap formation in nervous tissue and inhibit signal transfer. Nerve guides are used to bridge the neural gaps created as a result of these events. This study describes the design, construction, and testing of a nerve guide which carries inner guidance elements to provide an appropriate microenvironment for peripheral nerve regeneration. A methacrylated gelatin-poly(2-hydroxyethyl methacrylate) (GelMA-pHEMA) hydrogel and 3D pr...
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Permanent loss of neurologic function occurs when the central nervous system (CNS) is injured. Nerve regeneration is extremely limited because of dense scar tissue formation at the lesion site, especially in the spinal cord. For the treatment of this type of injury, different strategies are needed. The emerging approach to solve these problems is to provide a physical support via tubular bridging devices such as “nerve guides”. They are used to bridge the neural gaps in the treatment of spinal cord injury, ...
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Yucel, Deniz; Kose, Gamze Torun; Hasırcı, Vasıf Nejat (2010-12-01)
Injury of the nervous system, particularly in the spinal cord, impairs the quality of life of the patient by resulting in permanent loss of neurologic function. The main limitation in spinal cord regeneration is the lack of extracellular matrix to guide nerves for functional recovery of the transected nerve tissue. In the present study, a tissue engineered nerve tube was prepared by wrapping neural stem cells (NSCs) on aligned fibers using a micropatterned film with astrocytes aligned along the microgrooves...
Arslantunalı Şahin, Damla; Son, Çağdaş Devrim; Hasırcı, Vasıf Nejat; Department of Biotechnology (2022-2-1)
Any injury in peripheral nerves may result in a loss of neuronal communication along sensory and motor nerves, affecting patients’ daily activities. Today, there are various FDA approved commercial conduit materials; hollow tubes preventing them from used in gaps bigger than 10 mm, because they may lead axonal sprouts to form. The presented study includes pHEMA wrapping structure filled with GelMA-HaMA gel matrix as a nerve guidance channel. Following the structural analysis of the nerve guide, in vitro stu...
Citation Formats
T. DURSUN USAL, D. Yucel, and V. N. Hasırcı, “A novel GeIMA-pHEMA hydrogel nerve guide for the treatment of peripheral nerve damages,” INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, pp. 699–706, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32689.