Arslantunalı Şahin, Damla
Any injury in peripheral nerves may result in a loss of neuronal communication along sensory and motor nerves, affecting patients’ daily activities. Today, there are various FDA approved commercial conduit materials; hollow tubes preventing them from used in gaps bigger than 10 mm, because they may lead axonal sprouts to form. The presented study includes pHEMA wrapping structure filled with GelMA-HaMA gel matrix as a nerve guidance channel. Following the structural analysis of the nerve guide, in vitro studies were performed with Schwann cells seeded on pHEMA as support cells and it continued with SHSY5Y neuroblastoma cells seeded on the gel matrix. Tensile modulus and tensile strengths of pHEMA hydrogels were determined as 570.9 ± 92.13 and 501.8 ± 93.7 kPa, respectively. Although tensile strengths of the hydrogels were significantly smaller than the acellular nerve tissue, tensile modulus values were comparable to that of an acellular nerve tissue; showing that the conduits would be able to stay intact during nerve regeneration. In addition, 70% of SCs cells seeded were attached and increased their numbers 5 times in 4 weeks; showing the biocompatibility of the surfaces. GelMA-HaMA gel matrix, sacrificial inner part of the conduits, degraded completely in 5 weeks so that the regenerating cells were become able to penetrate the structure. As a result of CSLM studies with SHSY5Y cells, it was shown that neuroblastoma cells were able to attach and grow through the gel matrix with the help of SCs secreting nerve growth factors and Netrin-1 as a guidance.


A Bio and synthetic polymer based nerve guide tested under in vitro and in vivo conditions
Dursun Usal, Tuğba; Hasırcı, Vasıf Nejat; Department of Biotechnology (2019)
Damages to the peripheral nervous system due to age, diseases or trauma may lead to gap formation in nervous tissue and inhibit signal transfer. Nerve guides are used to bridge the neural gaps created as a result of these events. This study describes the design, construction, and testing of a nerve guide which carries inner guidance elements to provide an appropriate microenvironment for peripheral nerve regeneration. A methacrylated gelatin-poly(2-hydroxyethyl methacrylate) (GelMA-pHEMA) hydrogel and 3D pr...
Peripheral nerve conduits: Technology update
Hasırcı, Nesrin; Arslantunalı Şahin, Damla; Hasırcı, Vasıf Nejat (2014-12-01)
Peripheral nerve injury is a worldwide clinical problem which could lead to loss of neuronal communication along sensory and motor nerves between the central nervous system (CNS) and the peripheral organs and impairs the quality of life of a patient. The primary requirement for the treatment of complete lesions is a tension-free, end-to-end repair. When end-to-end repair is not possible, peripheral nerve grafts or nerve conduits are used. The limited availability of autografts, and drawbacks of the allograf...
Multiwalled CNT-pHEMA composite conduit for peripheral nerve repair.
Arslantunalı Şahin, Damla; Hasırcı, Vasıf Nejat (2014-03-01)
A nerve conduit is designed to improve peripheral nerve regeneration by providing guidance to the nerve cells. Conductivity of such guides is reported to enhance this process. In the current study, a nerve guide was constructed from poly(2-hydroxyethyl methacrylate) (pHEMA), which was loaded with multiwalled carbon nanotubes (mwCNT) to introduce conductivity. PHEMA hydrogels were designed to have a porous structure to facilitate the transportation of the compounds needed for cell nutrition and growth and al...
Design and in vitro testing of a peripheral nerve guide
Tamay, Dilara Göksu; Özen, Can.; Department of Biotechnology (2019)
Regeneration after peripheral nerve injury (PNI) is limited as chondroitin sulfate proteoglycans (CSPGs) within the scar tissue inhibit axon outgrowth. Nerve guides (NGs) are alternatives to the “gold standard” autografting, which has limitations including donor site morbidity and limited tissue availability. NGs are tubular constructs designed to form a bridge between the transected nerve ends, provide physical support, and achieve axon guidance via topographical and/or chemical cues. This study aims to de...
Study on the influence of scaffold inner organization on the treatment of spinal cord injury
Dursun, Tuğba; Hasırcı, Deniz; Yücel, Deniz; Department of Biotechnology (2013)
Permanent loss of neurologic function occurs when the central nervous system (CNS) is injured. Nerve regeneration is extremely limited because of dense scar tissue formation at the lesion site, especially in the spinal cord. For the treatment of this type of injury, different strategies are needed. The emerging approach to solve these problems is to provide a physical support via tubular bridging devices such as “nerve guides”. They are used to bridge the neural gaps in the treatment of spinal cord injury, ...
Citation Formats
D. Arslantunalı Şahin, “DEVELOPMENT OF A COMPOSITE GUIDE FOR PERIPHERAL NERVE REGENERATION,” Ph.D. - Doctoral Program, Middle East Technical University, 2022.