Influence of pH on recombinant human growth hormone production by Pichia pastoris

Date

2010-12-01

Author

Çalık, Pınar
Inankur, Bahar
Soyaslan, Elif S.
Sahin, Merve
Taspinar, Hatice
Acik, Eda
Yilmaz, Remziye
Ozdamar, Tuncer H.

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BACKGROUND: Effect of pH on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) was investigated at pH = 4.2, 5.0, 5.5, and 6.0.

Subject Keywords

Human growth hormone, AOX, Gene expression, Sorbitol, PH, Pichia pastoris

URI

https://hdl.handle.net/11511/32989

Journal

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

DOI

https://doi.org/10.1002/jctb.2474

Collections

Department of Chemical Engineering, Article

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Effects of pH strategy on endo- and exo-metabolome profiles and sodium potassium hydrogen ports of beta-lactamase-producing Bacillus licheniformis Ileri, N; Çalık, Pınar (Wiley, 2006-03-01) The effects of pH strategy on endo- and exo-metabolome profiling of beta-lactamase-producing Bacillus licheniformis were investigated at controlled-pH (pH(C) = 6.5, 6.75, 7.0, 7.25, 7.5) and uncontrolled-pH (pH(UC) = 7.5) values using a glucose-based defined medium. The cell concentration profiles were not affected by the pH considerably within the investigated range. The highest enzyme activities were obtained as A = 54 U cm(-3) at pHc = 6.75 among the controlled-pH operations and as A = 57 U cm(-3) at the...

Citation Formats

P. Çalık et al., “Influence of pH on recombinant human growth hormone production by Pichia pastoris,” JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, pp. 1628–1635, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32989.