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Influence of pH on recombinant human growth hormone production by Pichia pastoris
Date
2010-12-01
Author
Çalık, Pınar
Inankur, Bahar
Soyaslan, Elif S.
Sahin, Merve
Taspinar, Hatice
Acik, Eda
Yilmaz, Remziye
Ozdamar, Tuncer H.
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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BACKGROUND: Effect of pH on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) was investigated at pH = 4.2, 5.0, 5.5, and 6.0.
Subject Keywords
Human growth hormone
,
AOX
,
Gene expression
,
Sorbitol
,
PH
,
Pichia pastoris
URI
https://hdl.handle.net/11511/32989
Journal
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
DOI
https://doi.org/10.1002/jctb.2474
Collections
Department of Chemical Engineering, Article
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In this study, the effects of pH on therapeutically important protein, recombinant human erythropoietin (rhuEPO), production by Pichia pastoris was investigated at pH=4.0, 4.5, 5.0, 5.5 and 6.0. rHuEPO production was started by methanol induction in fed-batch mode. The highest cell concentration was obtained at pH=4.5 as 81.4 g L-1. The co-substrate substrate sorbitol, which was added batch-wise, was consumed at t=15 h of the operations at pH=4.0, 4.5 and 5.0. However as the pH increases above pH=5.0 the so...
Effects of ph on human growth hormone production by pichia pastoris considering the expression levels of regulatory genes
Bayraktar, Eda; Çalık, Pınar; Department of Chemical Engineering (2009)
In this study, the aim was to investigate the effects of pH on therapeutically important protein, recombinant human growth hormone (rhGH), production by Pichia pastoris considering the expression levels of regulatory genes. In this frame, firstly the host microorganism was selected between two different methanol utilization phenotypes of P. pastoris, Mut+ and MutS on media containing glycerol/methanol or sorbitol/methanol. The highest rhGH production, 120 g L-1, and hGH gene expression, 9.84x109 copies mg-1...
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Çalık, Pınar; Zerze, Gul H.; Inankur, Bahar; Bayraktar, Eda; Boy, Erdem; Acik, Eda; Ozdamar, Tuncer H. (2013-09-01)
BACKGROUNDEffects of co-substrate sorbitol different feeding strategies on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) were investigated by eight designed experiments grouped as: (i) fed-batch methanol feeding without the co-substrate; (ii) fed-batch methanol feeding with pulse sorbitol feeding; (iii) fed-batch methanol feeding together with fed-batch sorbitol feeding at t=0-15h, followed by fed-batch methanol feeding; and (iv) fed-batch methanol and sorbitol feeding at ...
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Ileri, N; Çalık, Pınar (Wiley, 2006-03-01)
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P. Çalık et al., “Influence of pH on recombinant human growth hormone production by Pichia pastoris,”
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
, pp. 1628–1635, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32989.