Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris

Date

2013-09-01

Author

Çalık, Pınar
Zerze, Gul H.
Inankur, Bahar
Bayraktar, Eda
Boy, Erdem
Acik, Eda
Ozdamar, Tuncer H.

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BACKGROUNDEffects of co-substrate sorbitol different feeding strategies on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) were investigated by eight designed experiments grouped as: (i) fed-batch methanol feeding without the co-substrate; (ii) fed-batch methanol feeding with pulse sorbitol feeding; (iii) fed-batch methanol feeding together with fed-batch sorbitol feeding at t=0-15h, followed by fed-batch methanol feeding; and (iv) fed-batch methanol and sorbitol feeding at t=0-30h, followed with fed-batch methanol feeding.

Subject Keywords

P.pastoris, Human growth hormone, Sorbitol, Methanol, Feeding rate , Feeding strategy

URI

https://hdl.handle.net/11511/47354

Journal

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

DOI

https://doi.org/10.1002/jctb.4011

Collections

Department of Chemical Engineering, Article

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Effects of ph on human growth hormone production by pichia pastoris considering the expression levels of regulatory genes Bayraktar, Eda; Çalık, Pınar; Department of Chemical Engineering (2009) In this study, the aim was to investigate the effects of pH on therapeutically important protein, recombinant human growth hormone (rhGH), production by Pichia pastoris considering the expression levels of regulatory genes. In this frame, firstly the host microorganism was selected between two different methanol utilization phenotypes of P. pastoris, Mut+ and MutS on media containing glycerol/methanol or sorbitol/methanol. The highest rhGH production, 120 g L-1, and hGH gene expression, 9.84x109 copies mg-1...
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Citation Formats

P. Çalık et al., “Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris,” JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, pp. 1631–1640, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/47354.