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Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris

2013-09-01
Çalık, Pınar
Bozkurt, Bahar
Zerze, Gul H.
Inankur, Bahar
Bayraktar, Eda
Boy, Erdem
Orman, Mehmet Ali
Acik, Eda
Ozdamar, Tuncer H.
BACKGROUNDEffects of co-substrate sorbitol different feeding strategies on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) were investigated by eight designed experiments grouped as: (i) fed-batch methanol feeding without the co-substrate; (ii) fed-batch methanol feeding with pulse sorbitol feeding; (iii) fed-batch methanol feeding together with fed-batch sorbitol feeding at t=0-15h, followed by fed-batch methanol feeding; and (iv) fed-batch methanol and sorbitol feeding at t=0-30h, followed with fed-batch methanol feeding.