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Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris
Date
2013-09-01
Author
Çalık, Pınar
Zerze, Gul H.
Inankur, Bahar
Bayraktar, Eda
Boy, Erdem
Acik, Eda
Ozdamar, Tuncer H.
Metadata
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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BACKGROUNDEffects of co-substrate sorbitol different feeding strategies on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) were investigated by eight designed experiments grouped as: (i) fed-batch methanol feeding without the co-substrate; (ii) fed-batch methanol feeding with pulse sorbitol feeding; (iii) fed-batch methanol feeding together with fed-batch sorbitol feeding at t=0-15h, followed by fed-batch methanol feeding; and (iv) fed-batch methanol and sorbitol feeding at t=0-30h, followed with fed-batch methanol feeding.
Subject Keywords
P.pastoris
,
Human growth hormone
,
Sorbitol
,
Methanol
,
Feeding rate
,
Feeding strategy
URI
https://hdl.handle.net/11511/47354
Journal
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
DOI
https://doi.org/10.1002/jctb.4011
Collections
Department of Chemical Engineering, Article
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In this study, the aim was to investigate the effects of pH on therapeutically important protein, recombinant human growth hormone (rhGH), production by Pichia pastoris considering the expression levels of regulatory genes. In this frame, firstly the host microorganism was selected between two different methanol utilization phenotypes of P. pastoris, Mut+ and MutS on media containing glycerol/methanol or sorbitol/methanol. The highest rhGH production, 120 g L-1, and hGH gene expression, 9.84x109 copies mg-1...
Influence of pH on recombinant human growth hormone production by Pichia pastoris
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In this study, it was aimed to investigate the effects of different co-carbon sources on therapeutically important glycoprotein, recombinant human erythropoietin (rHuEPO) production by Pichia pastoris by designing feeding strategies which were applied in the production phase of the bioprocess. During the experiments, the cell growth, sorbitol, mannitol, and methanol consumptions, recombinant human EPO production, alcohol oxidase activity, total protease concentrations and the by-products organic acid concen...
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In this study, the aim was to achieve high concentration of recombinant human growth hormone (rhGH) production by recombinant Pichia pastoris by investigating the effects of various operation parameters and to determine the suitable peptide ligand sequence that shows affinity and specificity to hGH. In this context, firstly the effect of temperature and Tween-20/80 addition on production and cell growth were investigated. While at T=30 and 32°C, there was no difference, at 27 and 25°C cell growth slowed dow...
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P. Çalık et al., “Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris,”
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
, pp. 1631–1640, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/47354.