Induced CYP1A mRNA, protein and catalytic activity in the liver of feral fish, leaping mullet, Liza saliens

Date

2001-02-01

Author

Arinc, E
Kocabıyık, Semra
Su, E

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

208
views

0
downloads

In this study, we examined whether levels of P4501A mRNA expression were naturally induced in feral fish, Liza saliens, and whether CYP1A protein levels and associated enzyme activity, EROD, were also increased. Induction of mRNA was measured using a nucleic acid hybridization technique. For the hybridization studies, a new 33-mer oligonucleotide probe 5 ' -dCTC ATC CAG CTT CCT GTC CTC GCA GTG ATC AAT-3 ' was designed, which corresponded to the totally conserved amino acid motif of CYP1A protein from positions 291 to 301 among the various fish species. Results of Northern blot analysis revealed that RNA isolated from the liver of mullet collected from the highly contaminated region of Izmir Bay with a dissolved and dispersed petroleum hydrocarbon content of 12.45 mug 1(-1) gave a strong hybridization signal, whereas only a weak hybridization signal was detected in the liver RNA of fish caught from the reference site containing less than 1 mug 1(-1) Of petroleum hydrocarbons. Similarly, fish from the contaminated site had approximately 80 times more EROD activity than the feral fish captured from the reference site. Studies using polyclonal antibodies produced against purified mullet CYP1A also showed the similar trend. In conclusion, feral leaping mullet caught from contaminated water displayed induction of CYP1A at three levels of expression, namely, mRNA, apoprotein and catalytic activity. (C) 2001 Elsevier Science Inc. All rights reserved.

Subject Keywords

Cytochrome, Ethoxyresorufin, O-deethylase (EROD), Antibodies, İnduction, Fish, Oligonucleotide probe, Mullet

URI

https://hdl.handle.net/11511/33158

Journal

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY

DOI

https://doi.org/10.1016/s1532-0456(01)00201-0

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Improvement of holdase activity of a small heat shock protein from extremely acidophilic archaea Kaçer, Yağmur; Kocabıyık, Semra; Department of Biochemistry (2019) In this study, a small heat shock protein gene (po-sHSP20) of archaeon Picrophilus oshimae which is the most thermoacidophilic organism known, was cloned and overexpressed in E.coli for the first time. The recombinant po-sHSP20 protein that was resistant to high temperature was purified to homogeneity by affinity chromatography. Oligomeric structure analysis of the po-sHSP20 by Size Exclusion Chromatography showed that it exists mostly as 12- and 18-mer oligomers with some dimeric/monomeric units under phys...
Regulatory gene effects on recombinant human growth hormone production by Bacillus subtilis Şahin, Merve; Çalık, Pınar; Özdamar, Tunçer H.; Department of Chemical Engineering (2010) In this study, regulatory gene effects on recombinant human growth hormone (rhGH) production by Bacillus subtilis were investigated. For this purpose, firstly Bacillus strains, which are deficient in abrB, aprE, degQ, degS, degU, scoC, sinI, sinR, and spo0A genes, were selected according to the regulatory gene network of aprE gene (serine alkaline protease gene of B. subtilis) since due to the degQ promoter and the pre-signal sequence of subC gene cloned in front of the hGH gene, hGH is produced by mimickin...
Genetic and morphometric variation in honeybee (Apis mellifera L.) populations of Turkey Kandemir, I; Kence, M; Kence, Aykut (Springer Science and Business Media LLC, 2000-05-01) Six enzyme systems were studied to determine the genetic variability in honeybee populations in Turkey. Ten morphometric characters were also measured to determine the extent of morphometric variation. Out of six enzyme systems, four were found to be polymorphic with 16 allozymes. The average heterozygosity was calculated as 0.072 +/- 0.007. Morphometric and electrophoretic variables were equally effective in discriminating honeybee populations. European and Anatolian honeybees were separated on the first a...
Alterations of ceramide/sphingosine 1-phosphate rheostat involved in the regulation of resistance to imatinib-induced apoptosis in K562 human chronic myeloid leukemia cells Baran, Yusuf; Salas, Arelis; Senkal, Can E.; Gündüz, Ufuk; Bielawski, Jacek; Obeid, Lina M.; Ogretmen, Besim (2007-04-13) In this study, mechanisms of resistance to imatinib-induced apoptosis in human K562 cells were examined. Continuous exposure to stepwise increasing concentrations of imatinib resulted in the selection of K562/IMA-0.2 and -1 cells, which expressed similar to 2.3- and 19-fold resistance, respectively. Measurement of endogenous ceramides by high performance liquid chromatography/mass spectroscopy showed that treatment with imatinib increased the generation of ceramide, mainly C-18-ceramide, which is generated ...
Site - Specific Mutations of Conserved C - Terminal Residues in Aminoglycoside 3'-Phosphotransferase II [APH(3')-II]: Phenotypic and Structural Analysis of the Mutant Enzymes Kocabıyık, Semra (1992-06-01) In order to test the biological importance of amino acids in the C-terminal quarter of aminoglycoside 3′-phosphotransferase II enzyme, seven of the highly conserved residues in this region, His-188, Asp-190, Asp-208, Gly-210, Arg-211, Asp-216 and Asp-220, were changed via site-directed mutagenesis. The phenotype of each mutant was compared to wildtype in terms of antibiotic susceptibilities and enzymatic activities. All of the substitutions either abolished or significantly reduced the resistance of the res...

Citation Formats

E. Arinc, S. Kocabıyık, and E. Su, “Induced CYP1A mRNA, protein and catalytic activity in the liver of feral fish, leaping mullet, Liza saliens,” COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, pp. 281–290, 2001, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/33158.