Hide/Show Apps

Transcriptional regulatory network refinement and quantification through kinetic modeling, gene expression microarray data and information theory

Sayyed-Ahmad, Abdallah
Tuncay, Kağan
Ortoleva, Peter J.
Background: Gene expression microarray and other multiplex data hold promise for addressing the challenges of cellular complexity, refined diagnoses and the discovery of well-targeted treatments. A new approach to the construction and quantification of transcriptional regulatory networks (TRNs) is presented that integrates gene expression microarray data and cell modeling through information theory. Given a partial TRN and time series data, a probability density is constructed that is a functional of the time course of transcription factor (TF) thermodynamic activities at the site of gene control, and is a function of mRNA degradation and transcription rate coefficients, and equilibrium constants for TF/gene binding.