Development of Random Amplified Polymorphic DNA (RAPD) markers in 150 F-6-inbred durum wheat (Triticum durum) lines derived from Kunduru-1149 x Cham-1 cross

Random Amplified Polymorphic DNA (RAPD) markers were developed in a specific durum wheat population (150 lines) derived from Kunduru-1149 x Chain-1 cross. Of 284 RAPD primers screened against parent DNAs, 83 revealed at least one polymorphic locus. The most polymorphic and clear bands producing 13 primers were selected and screened with the DNAs of 150 lines. These 13 primers revealed a total number of 33 segregating loci. The preliminary results of this study could be used as a base for mapping economically important character loci of durum wheat by the development of other RAPDs.


Development of random amplified polymorphic DNA markers for genetic mapping in Pacific yew (Taxus brevifolia)
Gocmen, B; Jermstad, KD; Neale, DB; Kaya, Zeki (Canadian Science Publishing, 1996-03-01)
A genetic linkage map was constructed for Pacific yew (Taxus brevifolia Nutt.) based on random amplified polymorphic DNA (RAPD) markers. A series of optimization experiments were conducted to develop a highly repeatable protocol for Pacific yew. In these experiments, a high MgCl2 concentration (5.5 mM) together with a low primer concentration (0.2 mu m) in the polymerase chain reaction (PCR) mixture yielded the best amplification products. PCR amplification products were further improved by treating the tem...
Application Of Esterase Polymorphism to Specıyf Population Genetic Structure of Engraulis Encrasicolus ( Pisces Engraulidae) in the Black and Azov Seas
İvanova, Pp; Dobrovolov, Is; Bat, Levent; Kıdeyş, Ahmet Erkan; Nikolsky, Vn; Yuneva, Tv; Shehepkina, Am; Ge, Shulman (2013-12-01)
Genetic structure of anchovy populations was studied using genetic-biochemical markers. Anchovy samples collected between 1980 and 2006 from different Black Sea regions (off the Bulgarian, Turkish, Ukraine, and Georgian coasts) as well as from the Sea of Azov were analyzed. Three methods of electrophoresis were applied: starch gel electrophoresis, vertical polyacrylamide electrophoresis, and isoelectric focusing on thin polyacrylamide ampholine gel plates. On the base of long-term monitoring of allelic freq...
Kaya, Zeki (1995-01-01)
We have applied the random amplified polymorphic DNA (RAPD) marker system to estimate linkage relationships in Pinus brutia TEN.. We used DNA samples from 30 haploid seed megagametophytes from each of 4 mother trees. Ninety-five 10-base oligonucleotide primers were evaluated and 34 revealed at least one polymorphic RAPD locus. The number of segregating RAPD loci per polymorphic primer varied from 2.32 to 3.25, but when segregating loci per tested primer was considered it was low, ranging on the average, fro...
Genome-wide exploration of metal tolerance protein (MTP) genes in common wheat (Triticum aestivum): insights into metal homeostasis and biofortification
Vatansever, Recep; Filiz, Ertugrul; Eroğlu, Seçkin (2017-04-01)
Metal transport process in plants is a determinant of quality and quantity of the harvest. Although it is among the most important of staple crops, knowledge about genes that encode for membrane-bound metal transporters is scarce in wheat. Metal tolerance proteins (MTPs) are involved in trace metal homeostasis at the sub-cellular level, usually by providing metal efflux out of the cytosol. Here, by using various bioinformatics approaches, genes that encode for MTPs in the hexaploid wheat genome (Triticum ae...
Identification of Physical Helicopter Models Using Subspace Identification
Avcioglu, Sevil; Kutay, Ali Türker; Leblebicioğlu, Mehmet Kemal (2020-04-01)
Subspace identification is a powerful tool due to its well-understood techniques based on linear algebra (orthogonal projections and intersections of subspaces) and numerical methods like singular value decomposition. However, the state space model matrices, which are obtained from conventional subspace identification algorithms, are not necessarily associated with the physical states. This can be an important deficiency when physical parameter estimation is essential. This holds for the area of helicopter ...
Citation Formats
B. Göçmen and Z. Kaya, “Development of Random Amplified Polymorphic DNA (RAPD) markers in 150 F-6-inbred durum wheat (Triticum durum) lines derived from Kunduru-1149 x Cham-1 cross,” ISRAEL JOURNAL OF PLANT SCIENCES, pp. 245–249, 2003, Accessed: 00, 2020. [Online]. Available: