UTILITY OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FOR LINKAGE MAPPING IN TURKISH RED PINE (PINUS-BRUTIA TEN)

Date

1995-01-01

Author

Kaya, Zeki

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

143
views

0
downloads

We have applied the random amplified polymorphic DNA (RAPD) marker system to estimate linkage relationships in Pinus brutia TEN.. We used DNA samples from 30 haploid seed megagametophytes from each of 4 mother trees. Ninety-five 10-base oligonucleotide primers were evaluated and 34 revealed at least one polymorphic RAPD locus. The number of segregating RAPD loci per polymorphic primer varied from 2.32 to 3.25, but when segregating loci per tested primer was considered it was low, ranging on the average, from 0.28 in tree 4 to 0.59 in tree 1. Based on the RAPD loci segregating in 1:1 ratio, genetic Linkage groups formed from 6 for genotype-l (total map distance=163.91 cM) to 13 for genotype-3 (total map distance=511.2 cM). It was also found that a number of segregating loci in all 4 genotypes (ranging from 14 to 21) could not be assigned into any of the constructed linkage groups. It was difficult to compare linkage groups among the genotypes since most of RAPD loci segregating in one genotype were not found in others. Thus, the linkage map provided very little information on the genomic organization of RAPD markers at the species level. The utility of RAPD markers in forest genetics is also discussed in the paper.

Subject Keywords

PINUS BRUTIA, RAPD MARKERS, GENETIC LINKAGE MAPPING, POLYMERASE CHAIN REACTION

URI

https://hdl.handle.net/11511/53246

Journal

SILVAE GENETICA

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Genetic differentiation of Abies equi-trojani (Asch. & Sint. ex Boiss) Mattf. populations from Kazdaǧι, Turkey and the genetic relationship between Turkish firs belonging to the Abies nordmanniana Spach complex Kaya, Zeki; Neale, David Brian (2008-02-05) The present study aimed to test the utility of RAPD (randomly amplified polymorphic DNA) and cpSSR (simple sequence repeats) markers for in situ gene conservation programs for fir species, as well as for determining the genetic similarities between the Abies nordmanniana Spach species complex (A. nordmanniana, A. bornmuelleriana Matff., A. equi-trojani (Asch. & Sint. ex Boiss) Mattf.) and between populations of A. equi-trojani, which is a narrow-endemic to Turkey. For this purpose, DNA was extracted and poo...
MOLECULAR ANALYSES OF THE GENERA EREMOPYRUM (LEDEB.) JAUB. & SPACH AND AGROPYRON GAERTNER (POACEAE) BY PCR METHODS Yilmaz, Remziye; CABİ, EVREN; Doğan, Musa (2014-06-01) RAPD-PCR (Random Amplified Polymorphic DNA Polymerase Chain Reaction) and Post PCR (Polymerase Chain Reaction) Melting Curve Analysis (MCA) have been used to investigate the pattern of genetic variation among some species in the genera Eremopyrum (Ledeb.) Jaub. & Spach and Agropyron Gaertner (Poaceae). Thirteen primers have been used in the study based on the RAPD-PCR and MCA analyses. Each species produced a distinct pattern of DNA fragments which have been used as a measure of the degree of relationship b...
Applications of estimation techniques on genetic and other types of data Aslan, Murat; Akkaya, Ayşen; Department of Statistics (2003) The parameters of genetic and other types of data, particularly with small samples, are estimated by using method of moments, least squares, minimum chi- square, maximum likelihood and modified maximum likelihood estimation methods. These methods are also compared in terms of their efficiencies and robustness property.
Employing DNA barcoding as taxonomy and conservation tools for fish species censuses at the southeastern Mediterranean, a hot-spot area for biological invasion Karahan, Arzu; PAZ, Guy; STERN, Nir; Kıdeyş, Ahmet Erkan; Goren, Menachem; RİNKEVİCH, Baruch (2017-04-01) This study evaluates the utility of DNA barcoding (mitochondrial cytochrome oxidase subunit I; COI) as a biodiversity and conservation applied tool for identifying fish fauna from the southeastern Mediterranean (the continental coast of Israel), a hot-spot area for biological invasion, also with an eye to establish the foundation for follow-up studies that will use environmental DNA (eDNA) tracks of native and invasive species, and for the application of eDNA concepts and methodologies in nature conservatio...
Stability of (N+1) -body fermion clusters in a multiband Hubbard model Iskin, M.; Keleş, Ahmet (2022-9-01) We start with a variational approach and derive a set of coupled integral equations for the bound states of N identical spin-↑ fermions and a single spin-↓ fermion in a generic multiband Hubbard Hamiltonian with an attractive on-site interaction. As an illustration, we apply our integral equations to the one-dimensional sawtooth lattice up to N≤3, i.e., to the (3+1)-body problem, and we reveal not only the presence of tetramer states in this two-band model but also their quasiflat dispersion when formed in...

Citation Formats

Z. Kaya, “UTILITY OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FOR LINKAGE MAPPING IN TURKISH RED PINE (PINUS-BRUTIA TEN),” SILVAE GENETICA, pp. 110–116, 1995, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53246.