We have applied the random amplified polymorphic DNA (RAPD) marker system to estimate linkage relationships in Pinus brutia TEN.. We used DNA samples from 30 haploid seed megagametophytes from each of 4 mother trees. Ninety-five 10-base oligonucleotide primers were evaluated and 34 revealed at least one polymorphic RAPD locus. The number of segregating RAPD loci per polymorphic primer varied from 2.32 to 3.25, but when segregating loci per tested primer was considered it was low, ranging on the average, from 0.28 in tree 4 to 0.59 in tree 1. Based on the RAPD loci segregating in 1:1 ratio, genetic Linkage groups formed from 6 for genotype-l (total map distance=163.91 cM) to 13 for genotype-3 (total map distance=511.2 cM). It was also found that a number of segregating loci in all 4 genotypes (ranging from 14 to 21) could not be assigned into any of the constructed linkage groups. It was difficult to compare linkage groups among the genotypes since most of RAPD loci segregating in one genotype were not found in others. Thus, the linkage map provided very little information on the genomic organization of RAPD markers at the species level. The utility of RAPD markers in forest genetics is also discussed in the paper.


Genetic differentiation of Abies equi-trojani (Asch. & Sint. ex Boiss) Mattf. populations from Kazdaǧι, Turkey and the genetic relationship between Turkish firs belonging to the Abies nordmanniana Spach complex
Kaya, Zeki; Neale, David Brian (2008-02-05)
The present study aimed to test the utility of RAPD (randomly amplified polymorphic DNA) and cpSSR (simple sequence repeats) markers for in situ gene conservation programs for fir species, as well as for determining the genetic similarities between the Abies nordmanniana Spach species complex (A. nordmanniana, A. bornmuelleriana Matff., A. equi-trojani (Asch. & Sint. ex Boiss) Mattf.) and between populations of A. equi-trojani, which is a narrow-endemic to Turkey. For this purpose, DNA was extracted and poo...
Yilmaz, Remziye; CABİ, EVREN; Doğan, Musa (2014-06-01)
RAPD-PCR (Random Amplified Polymorphic DNA Polymerase Chain Reaction) and Post PCR (Polymerase Chain Reaction) Melting Curve Analysis (MCA) have been used to investigate the pattern of genetic variation among some species in the genera Eremopyrum (Ledeb.) Jaub. & Spach and Agropyron Gaertner (Poaceae). Thirteen primers have been used in the study based on the RAPD-PCR and MCA analyses. Each species produced a distinct pattern of DNA fragments which have been used as a measure of the degree of relationship b...
Applications of estimation techniques on genetic and other types of data
Aslan, Murat; Akkaya, Ayşen; Department of Statistics (2003)
The parameters of genetic and other types of data, particularly with small samples, are estimated by using method of moments, least squares, minimum chi- square, maximum likelihood and modified maximum likelihood estimation methods. These methods are also compared in terms of their efficiencies and robustness property.
Employing DNA barcoding as taxonomy and conservation tools for fish species censuses at the southeastern Mediterranean, a hot-spot area for biological invasion
Karahan, Arzu; PAZ, Guy; STERN, Nir; Kıdeyş, Ahmet Erkan; Goren, Menachem; RİNKEVİCH, Baruch (2017-04-01)
This study evaluates the utility of DNA barcoding (mitochondrial cytochrome oxidase subunit I; COI) as a biodiversity and conservation applied tool for identifying fish fauna from the southeastern Mediterranean (the continental coast of Israel), a hot-spot area for biological invasion, also with an eye to establish the foundation for follow-up studies that will use environmental DNA (eDNA) tracks of native and invasive species, and for the application of eDNA concepts and methodologies in nature conservatio...
Analysis of genetic relationships among perennial and annual Cicer species growing in Turkey using RAPD markers
Sudupak, MA; Akkaya, Mahinur; Kence, A (2002-12-01)
Random amplified polymorphic DNA (RAPD) fragments were used to assess genetic relationships among Cicer spp. growing in Turkey. Seven 10-mer primers selected from a 50 random oligonucleotide primer set, depending on their ability to amplify genomic DNA in all species, were used to detect RAPD variation in 43 wild and cultivated accessions representing ten species. These primers yielded 95 reproducible amplification products, 92 of which were polymorphic. Pairwise,genetic distances of accessions estimated ac...
Citation Formats
Z. Kaya, “UTILITY OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FOR LINKAGE MAPPING IN TURKISH RED PINE (PINUS-BRUTIA TEN),” SILVAE GENETICA, pp. 110–116, 1995, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53246.